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  • The Nuclear Membrane

    Methods and Protocols

    Edición de Yuki Hara, Kazunori Kume ...
    Series series Springer Protocols
    This book compiles protocols that encompass a diverse range of classical and contemporary approaches, including biochemical techniques, proteomic analyses, microscopic observations, and biophysical measurements. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions tothe material, lists of necessary materials and reagents, step-by ... Leer más

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  • Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology

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    Bringing this best-selling textbook right up to date, the new edition uniquely integrates the theories and methods that drive the fields of biology, biotechnology and medicine, comprehensively covering both the techniques students will encounter in lab classes and those that underpin current key advances and discoveries. The contents have been updated to include both traditional and cutting-edge ... Leer más

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  • Molecular Motors

    Methods and Protocols

    Series series Springer Protocols
    This fully updated volume presents a diverse collection of protocols and methodologies for studying various molecular motors. The book explores the dynamics of the bacterial flagellar motor, the mechanisms of ATP synthase, the assembly and function of cytoskeletal motors, and the role of nucleic acid motors in cellular processes, while placing a strong emphasis on state-of-the-art techniques, ... Leer más

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  • The Mitotic Spindle

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    Edición de Tomomi Kiyomitsu ...
    Series series Springer Protocols
    This detailed volume presents methods for investigating mitotic spindle assembly, chromosome segregation, and cytokinesis. Split into six parts, the book examines advanced microscopes, spatiotemporal manipulation of the spindle and target molecules, quantitative live imaging, screening, centrosome manipulation and purification, and spindle positioning in synthetic or complicated contexts. Written ... Leer más

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  • Chromosome Architecture

    Methods and Protocols

    Edición de Mark C. Leake ...
    Series series Springer Protocols
    This detailed new edition collects cutting-edge laboratory protocols, techniques, and applications in use by some of the leading international experts in the broad field of chromosome architecture. The book emphasizes the increasing physiological relevance of chromosome architecture investigation, manifest both through application of more complex bottom-up assays in vitro as well as through ... Leer más

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  • SNAREs

    Methods and Protocols

    Edición de Rutilio Fratti ...
    Series series Springer Protocols
    This second edition details new and updated methods on soluble N-ethylmaleimide-sensitive factor attachment protein receptors( SNAREs) and their function are examined in the laboratory. Chapters guide readers through an overview of the basic properties of SNAREs, distribution and interaction with regulators of membrane fusion, activation of SNAREs in the priming stage by NSF/Sec18 and a-SNAP/Sec17 ... Leer más

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  • Iron Metabolism

    Methods and Protocols

    Edición de Oleh Khalimonchuk ...
    Series series Springer Protocols
    This detailed volume explores classical and cutting-edge methods optimized and validated to analyze various aspects of iron metabolism, from in vitro to multi-organ level. Opening with a section on basic iron metabolism methods, the book continues with methods applicable to a variety of systems, ranging from bacteria to cultured mammalian cells and tissues, with a focus on cellular heme and iron ... Leer más

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  • Photorespiration

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    Edición de Berkley J Walker ...
    Series series Springer Protocols
    This volume provides methods on researching photorespiration to provide a better understanding of this vital pathway in plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting ... Leer más

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    Edición de Andrzej Stanisław Cieplak ...
    Series series Springer Protocols
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  • Liposomes

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    This third edition provides new and updated chapters detailing preparation of liposomes, physicochemical characterization of liposomes, lipid analysis, drug encapsulation, surface modification, stimuli response as well as cellular interaction, and biodistribution. Also included is an updated chapter on the history and evolution of the field of liposomology. Written in the format of the highly ... Leer más

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  • Dynein

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    Edición de Steven M. Markus ...
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    This volume provides a collection of protocols from leading researchers in the dynein field. The enclosed chapters guide readers through a broad range of powerful techniques that can be used to understand the complexities of dynein activity and regulation. These include how to perform live-cell microscopy on various fungi and metazoan cells, how to purify, assemble, and measure the force ... Leer más

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