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  • Phytophthora

    Methods and Protocols

    Series series Springer Protocols
    This comprehensive volume examines techniques for studying Phytophthora species, a devastating group of plant pathogens that cause diseases in crops and natural ecosystems worldwide. The book compiles essential molecular techniques and methods, such as CRISPR-Cas genome editing for investigating pathogenicity and resistance, as well as tools for detecting, visualizing, and quantifying disease. ... Leer más

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  • Biotechnologies for Plant Mutation Breeding

    Protocols

    This book is open access under a CC BY-NC 2.5 license.This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular ... Leer más

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  • Catharanthus roseus

    Methods and Protocols

    Series series Springer Protocols
    This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in C. roseus from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, transcription factor characterization up to functional genomic tools based on gene overexpression. Written in the format of the highly successful Methods in ... Leer más

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  • Drosophila

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    Edición de Christian Dahmann ...
    Series series Springer Protocols
    This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and ... Leer más

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  • Zebrafish

    Methods and Protocols

    Series series Springer Protocols
    This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the ... Leer más

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  • Molecular Tools for the Detection and Quantification of Toxigenic Cyanobacteria

    A guide to state-of-the-art molecular tools for monitoring and managing the toxigenicity of cyanobacteriaRunaway eutrophication and climate change has made the monitoring and management of toxigenic organisms in the world’s bodies of water more urgent than ever. In order to influence public policy regarding the detection and quantification of those organisms, it is incumbent upon scientists to ... Leer más

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  • Flower Development

    Methods and Protocols

    Series series Springer Protocols
    This second edition details new and updated protocols for experimental approaches that are currently used to study the formation of flowers. Chapters guide readers on genetic methods, phenotypic analyses, genome-wide experiments, modeling, and system-wide approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective ... Leer más

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  • Phytoplasma

    Methods and Protocols

    Edición de Katrin Janik, Mattia Tabarelli ...
    Series series Springer Protocols
    This volume provides a comprehensive understanding of the methods used in studying phytoplasma diseases and transmission. Chapters cover a wide range of topics, from basic detection methods to advanced genomics and emerging technologies, vectors, plant interactions, and disease management. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions ... Leer más

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  • Plant Stress Tolerance

    Methods and Protocols

    Edición de Ramanjulu Sunkar ...
    Series series Springer Protocols
    This fully updated new edition explores new techniques for studying plant stress. This includes novel methodologies such as MeRIP-seq for identifying changes in m6A profiles, isolation of stress granules, and additional methodologies such as MNase–seq for identifying nucleosome occupancy, alternative splicing analysis, identifying proteins that interact with long noncoding RNAs, untargeted ... Leer más

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  • Metagenomics

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    Edición de Wolfgang R. Streit, Rolf Daniel ...
    Series series Springer Protocols
    This volume provides up-to-date and novel techniques for various screening technologies currently used in metagenomics and related areas. Starting with DNA/RNA isolation from environmental samples, the book continues by delving into areas such as current methods used to isolate DNA and construct metagenomic libraries, establishment of metagenome libraries in non-E. coli hosts, and topics like ... Leer más

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  • Schizosaccharomyces pombe

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    Edición de Ruben C. Petreaca ...
    Series series Springer Protocols
    This detailed volume explores techniques utilizing the fission yeast Schizosaccharomyces pombe, a powerful tractable model system for eukaryotic biology. The book begins with a section on microscopy and imaging, and it continues with chapters covering genome and plasmid editing, including the use of CRISPR/Cas9 technologies, as well as the analysis of DNA damage, repair, and recombination, and ... Leer más

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  • Heterologous Expression of Membrane Proteins

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    Edición de Isabelle Mus-Veteau ...
    Series series Springer Protocols
    This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus ... Leer más

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