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  • Stem Cell-Based Human Embryo Models

    Methods and Protocols

    Edición de Vincent Pasque, Kursad Turksen ...
    Series series Springer Protocols
    This detailed volume covers diverse aspects of stem cell research and exemplifies some of the developments and improvements in the field. The book explores how to regulate and manipulate stem cells appropriately in vitro, allowing for the generation of very early embryo-like structures, known as blastoids, opening up opportunities for analyzing and delineating the very early steps in embryo ... Leer más

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  • The AGT Cytogenetics Laboratory Manual

    Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics.Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis ... Leer más

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  • Zebrafish

    Methods and Protocols

    Series series Springer Protocols
    This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the ... Leer más

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  • Drosophila

    Methods and Protocols

    Edición de Christian Dahmann ...
    Series series Springer Protocols
    This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and ... Leer más

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  • Embryo Models In Vitro

    Methods and Protocols

    Series series Springer Protocols
    This collection explores the use of pluripotent cells to generate early embryo-like structures in vitro, with the resultant 3D embryo-like structures in vitro phenocopying many of the developmental landmarks and 3D architectures seen normally in vivo. The protocols gathered herein cover diverse aspects of the topic and exemplify some of the key developments and improvements in the field. Written ... Leer más

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  • Embryonic Stem Cell Protocols

    Edición de Kursad Turksen ...
    Series series Springer Protocols
    This fully updated volume explores improved and extended protocols for embryonic stem cell (ESC) research, provided with the most direct and informative methodologies currently available. The book examines how these models for cell lineage and differentiation studies have continued to mature into a critical research workhorse. Written for the highly successful Methods in Molecular Biology series ... Leer más

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  • Spermatogenesis

    Methods and Protocols

    Edición de Prabhakara P. Reddi ...
    Series series Springer Protocols
    This volume presents a comprehensive collection of research methods at the cellular, molecular, and biochemical level to understand sperm production and function. Exploring spermatogenesis and testis biology, the book delves into epigenomics and transcriptomics, spermatogenic cell separation, identification of cell types and stages, and more. Written for the highly successful Methods in Molecular ... Leer más

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  • Cell Migration in Three Dimensions

    Edición de Coert Margadant ...
    Series series Springer Protocols
    This detailed collection serves as a unique and excellent collection of state-of-the-art methods and protocols to interrogate cell migration in a wide variety of different contexts and model organisms, as well as advanced image analysis and quantitative assessment of a diverse array of parameters related to cell migration. The book focuses on the cell biology of cell migration, developmental model ... Leer más

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  • Germ Cell Development

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    Edición de Marco Barchi, Massimo De Felici ...
    Series series Springer Protocols
    This detailed volume explores techniques for the isolation, purification, and establishment of in vitro germ cell (GS) systems at different stages of development in a variety of organisms, including chickens, mice, rats, and humans. The book additionally describes cutting-edge analytic and informatic tools to study germ cell development at the single cell level and meiotic recombination. Written ... Leer más

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  • Aspergillus fumigatus

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    Edición de Natalia Mach ...
    Series series Springer Protocols
    This book presents current molecular biology methods involving Aspergillus fumigatus for researchers in mycology, fungal genetics, and medical microbiology looking to deepen their understanding of this species of fungus at the molecular level. Sections of the book explore approaches to CRISPR-based genome editing, methods for identification, genotyping, and detection, assays for gene regulation, ... Leer más

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  • Drosophila Oogenesis

    Methods and Protocols

    Series series Springer Protocols
    This detailed volume compiles numerous methods to explore fly oogenesis. Beginning with updated protocols from isolating and staining the ovary to numerous imaging techniques and genetic protocols for cell-specific assessment and CRISPR-mediated mutagenesis, the volume continues by detailing techniques from assessing cytoskeletal structures to uncovering protein-protein interactions, with closing ... Leer más

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  • Skeletal Muscle Stem Cells

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    Edición de Atsushi Asakura ...
    Series series Springer Protocols
    This volume looks at the latest technologies and methods--combined with new genetic tools available in animal models--used in this constantly evolving field. The chapters in this book are organized into three sections: Section one covers muscle stem cells and progenitor cells; Section Two discusses animal models for muscle stem cells and regeneration; and Section Three explores bioinformatics and ... Leer más

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