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RNA Analysis

Methods and Protocols

Edición de Laurent Delpy ...

Series series Springer Protocols
This volume explores the latest technologies for RNA analysis, covering fundamental techniques and innovative approaches that expand our understanding of RNA biology. The chapters in this book are organized into four parts: reverse transcription and RNA capture methods; high-throughput sequencing methods; RNA maturation: splicing, polyadenylation, and epitranscriptomic modifications; and RNA ... Leer más

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This volume explores the latest technologies for RNA analysis, covering fundamental techniques and innovative approaches that expand our understanding of RNA biology. The chapters in this book are organized into four parts: reverse transcription and RNA capture methods; high-throughput sequencing methods; RNA maturation: splicing, polyadenylation, and epitranscriptomic modifications; and RNA
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Drosophila

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Edición de Christian Dahmann ...

Series series Springer Protocols
This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and ... Leer más

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This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and
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Chromoanagenesis

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Edición de Franck Pellestor ...

Series series Springer Protocols
This extensive volume explores the phenomena of chaotic genomic rearrangements, known as chromoanagenesis, their formation mechanisms, and the state-of-the-art techniques used to detect them. The book delves into the potential impact of chromoanagenesis on cancers and congenital diseases, as well as its broader implications for genomic evolution and human health through a variety of cutting-edge ... Leer más

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This extensive volume explores the phenomena of chaotic genomic rearrangements, known as chromoanagenesis, their formation mechanisms, and the state-of-the-art techniques used to detect them. The book delves into the potential impact of chromoanagenesis on cancers and congenital diseases, as well as its broader implications for genomic evolution and human health through a variety of cutting-edge
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Cell Cycle Control

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Series series Springer Protocols
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Bacterial Secretion Systems

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Edición de Laure Journet, Eric Cascales ...

Series series Springer Protocols
This second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or ... Leer más

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This second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or
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Bacterial Regulatory RNA

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This second edition details new and updated methods used for studying prokaryotic non-coding RNAs and their protein accomplices. Chapters detail discovery of ncRNAs, characterization of their structure, functions, and their interactomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary ... Leer más

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Recombinant Protein Expression in Mammalian Cells

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Edición de David L. Hacker ...

Series series Springer Protocols
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This second edition volume discusses the latest techniques and protocols used in the field that were not covered in the previous edition. The chapters in this book are organized into five parts. Part One looks at transcriptomic analyses and Part Two covers DNA replication and protein/DNA interactions. Part Three discusses translation dynamics, protein complexes, and proteomics. Part Four looks at ... Leer más

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Series series Springer Protocols
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Series series Springer Protocols
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This volume provides a comprehensive collection of protocols that can be used to study plant chromatin structure and composition. Chapters divided into five sections detail the profiling of chromatin features in relation to epigenetic regulation, investigate the interaction between chromatin modifications and gene regulation, and explore the 3D spatial organization of the chromatin inside the
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Series series Springer Protocols
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Chromoanagenesis

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Bacterial Regulatory RNA

Recombinant Protein Expression in Mammalian Cells

Yeast Functional Genomics

mRNA Decay

Genotype Phenotype Coupling

Methods for Plant Nucleus and Chromatin Studies

DNA-Protein Interactions