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  • Protein Arrays

    Methods and Protocols

    Series series Springer Protocols
    This volume discusses the latest methods and applications of protein microarrays and helps researchers harness its power in their work. The chapters in this book explore protein microarray’s development and fabrication, strategies for their data analysis, and wide-reaching applications in biology and disease research. Written in the highly successful Methods in Molecular Biology series format, ... Leer más

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  • IMMUNO-model in Cancer

    Methods and Protocols

    Edición de Sweta Rani, Lukasz Skalniak ...
    Series series Springer Protocols
    This open access volume describes the computational and experimental models that help researchers understand the responses of the immune system in cancer and test experimental immune-oncology approaches. The chapters in this book cover methods such as profile polarization in macrophages using ELISA; immune checkpoint inhibitors; interactions of PD-1/PD-L1 and T cells; immunocompetent preclinical ... Leer más

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  • Mass Spectrometry-Based Proteomics

    Edición de Kris Gevaert ...
    Series series Springer Protocols
    This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by ... Leer más

    $182,769 CLP

  • Cancer Immunosurveillance

    Methods and Protocols

    Series series Springer Protocols
    This new edition explores the innovation and improvement of experimental techniques harnessed to dissect the complex relationship between cancer cells and the immune system of the host. Beginning with spectral and multicolor flow cytometric methods to characterize the immune contexture of different forms of metastases, the book continues with functional assays to study the activation status of ... Leer más

    $217,286 CLP

  • Peptide Microarrays

    Methods and Protocols

    Edición de Marina Cretich, Alessandro Gori ...
    Series series Springer Protocols
    This detailed volume provides an updated overview of current uses of peptide microarray technology, showcasing consolidated applications while highlighting some of the most intriguing novelties and emerging fields of use. The methodologies within this collection are of considerable value for both advanced users and new-comers in the peptide microarray arena, as the renowned contributors describe ... Leer más

    $108,636 CLP

  • Serum/Plasma Proteomics

    Methods and Protocols

    Series series Springer Protocols
    This third volume provides comprehensive protocols on pre-analytical, analytical, plasma, and serum proteomics. New and updated chapters are divided into nine sections, detailing blood processing and handling strategies, discovery- and targeted-based mass spectrometry, including workflows to aid in discovery and targeted data analysis, in addition to software and bioinformatics for the plasma ... Leer más

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  • Regulatory T-Cells

    Methods and Protocols

    Edición de Masahiro Ono ...
    Series series Springer Protocols
    This volume provides methods and models for investigating the immunosuppressive subset of CD4+ T-cells, regulatory T-cells (Treg). Chapters guide readers through, basic protocols for analyzing Treg in mice and humans, single-cell analysis for analyzing Treg, and methods for the analysis of Treg in clinical application. Written in the format of the highly successful Methods in Molecular Biology ... Leer más

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  • Brown Adipose Tissue

    Methods and Protocols

    Series series Springer Protocols
    This detailed volume explores techniques for researching brown adipose tissue (BAT) and the fascinating biology and therapeutic potential of thermogenic adipocytes. The content reflects the advancing technologies in genetics, imaging, and 'omics strategies that are allowing researchers to probe BAT biology at unprecedented depths and detail, yet it also presents classic physiology principles, ... Leer más

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  • Proteoglycans

    Methods and Protocols

    Edición de Nikos K. Karamanos ...
    Series series Springer Protocols
    This volume is acomprehensive and up-to-date collection of strategies, reproducible methods, and protocols for the in-depth analysis of Proteoglycans (PGs) and their glycan part, the GAGs. Chapters are divided into three parts detailing GAGs in biological specimens, protocols for the evaluation of the in vitro and in vivo effects of PGs/GAGs, and protocols for compounds related with the metabolic ... Leer más

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  • NLR Proteins

    Methods and Protocols

    Series series Springer Protocols
    This second edition provides a sound basis for the molecular investigation of NLR function in health and disease. Chapters focus on NLR as innate immune receptors, “atypical” inflammasomes, biochemical and novel bioluminescence techniques for the measurement of IL-1b as readout of NLR-based inflammasomes, bioluminescent probe, biochemical and microscopy techniques, techniques to measure caspase-1 ... Leer más

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  • Signal Transduction Immunohistochemistry

    Methods and Protocols

    Edición de Alexander E. Kalyuzhny ...
    Series series Springer Protocols
    This new edition collects diverse protocols compiled by experts from different areas of research as well as by biotech researchers developing novel technologies in the area of immunohistochemistry (IHC). After a few chapters to help orient novices entering the biomedical arena, the book includes methods chapters covering multiplex IHC including fluorescence and chromogenic techniques, combination ... Leer más

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  • Induced Pluripotent Stem (iPS) Cells

    Methods and Protocols

    Edición de Andras Nagy, Kursad Turksen ...
    Series series Springer Protocols
    This extensive new edition presents protocols reflecting the great strides made in the study of induced pluripotent stem (iPS) cells. The collection explores new and improved methods for the generation, expansion, and maintenance of iPS cells from different tissue types, characterization of their differentiation pathways along different lineages, and their potential utility in tissue repair and ... Leer más

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