Cos'hanno in comune un aforisma di Yogi Berra, giocatore di baseball americano, e la leggendaria lettura tenuta al Caltech nel 1959 da Richard Feynman? "Basterebbe osservare!", esortano entrambi. "Basterebbe osservare" per capire il vivente, come si fa con un microscopio. L'occhio permette un dettaglio del decimo di millimetro ma la vita si svolge sulla scala del miliardesimo di metro tra DNA e proteine, cellule, tessuti e organi. Con un pezzo di vetro curvo e i colori dell'arcobaleno si p...

The story of microscopy over the years is one of wonder, revelation, and even love. What better words could there be to describe the amazing things that we have been able to see, learn and accomplish thanks to the progress made in this field? A love story between a pieace of glass and the rainbow with an original soundtrack mad of poetry and music.From Galilei’s initial foray into basic optical microscopy, including the Camillo Golgi and Giuliano Toraldo di Francia lessons, to such...

This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors.The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in ...

"Alberto Diaspro has been choreographing light's dance for over 20 years, and in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, he has assembled a diverse group of experts to explain the methods they use to coax light to reveal biology's secrets."- From the Foreword by Daniel Evanko, editor, Nature Methods Nanoscopy and Multidimens

In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world...

This book encompasses the full breadth of the super-resolution imaging field, representing modern techniques that exceed the traditional diffraction limit, thereby opening up new applications in biomedicine. It shows readers how to use the new tools to increase resolution in sub-nanometer-scale images of living cells and tissue, which leads to new information about molecules, pathways and dynamics. The book highlights the advantages and disadvantages of the techniques, and gives state-of-t...