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  • DNA-Encoded Chemical Libraries

    Methods and Protocols

    Edición de David Israel, Yun Ding ...
    Series series Springer Protocols
    This volume discusses protocols that cover synthesis, screening by selection, and analysis of DNA-encoded chemical libraries (DEL). Chapters in this book focus on methods used to practice DEL technology and include solution phase library synthesis using a variety of chemistries; DNA encoding of chemical structure; design, preparation and analysis of target proteins and tool compounds; screening of ... Leer más

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  • Introducing Proteomics

    From Concepts to Sample Separation, Mass Spectrometry and Data Analysis

    de Josip Lovric ...
    Introducing Proteomics gives a concise and coherent overview of every aspect of current proteomics technology, which is a rapidly developing field that is having a major impact within the life and medical sciences.This student-friendly book, based on a successful course developed by the author, provides its readers with sufficient theoretical background to be able to plan, prepare, and analyze a ... Leer más

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  • Catharanthus roseus

    Methods and Protocols

    Series series Springer Protocols
    This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in C. roseus from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, transcription factor characterization up to functional genomic tools based on gene overexpression. Written in the format of the highly successful Methods in ... Leer más

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  • Downstream Industrial Biotechnology

    Recovery and Purification

    Edición de Michael C. Flickinger ...
    DOWNSTREAM INDUSTRIAL BIOTECHNOLOGYAn affordable, easily accessible desk reference on biomanufacturing, focused on downstream recovery and purificationAdvances in the fundamental knowledge surrounding biotechnology, novel materials, and advanced engineering approaches continue to be translated into bioprocesses that bring new products to market at a significantly faster pace than most other ... Leer más

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  • DNA Origami

    Structures, Technology, and Applications

    de Masayuki Endo ...
    DNA ORIGAMIDiscover the impact and multidisciplinary applications of this subfield of DNA nanotechnologyDNA origami refers to the technique of assembling single-stranded DNA template molecules into target two- and three-dimensional shapes at the nanoscale. This is accomplished by annealing templates with hundreds of DNA strands and then binding them through the specific base-pairing of ... Leer más

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  • Fluorescent Proteins

    Methods and Protocols

    Edición de Mayank Sharma ...
    Series series Springer Protocols
    This volume brings together cutting-edge laboratory protocols to characterize the novel fluorescent proteins (FPs) and approaches based on fluorescent proteins that aim to answer some of the key cell biological questions. The book covers topics ranging from the database of fluorescent proteins to their characterization and adaptation to a wide range of biological systems. Written for the highly ... Leer más

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  • MicroRNA Detection and Target Identification

    Methods and Protocols

    Edición de Tamas Dalmay ...
    Series series Springer Protocols
    This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe ... Leer más

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  • Nucleic Acid Aptamers

    Selection, Characterization, and Application

    Edición de Günter Mayer, Marcus M. Menger ...
    Series series Springer Protocols
    This updated book reflects improvements in a variety of techniques used to study the aptamer field. Beginning with a section on selection procedures, the volume continues with methods to characterize aptamers’ interaction and structural properties by biophysical approaches, as well as a variety of applications that have been adapted to the aptamer compound class. Written for the highly successful ... Leer más

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  • Protein Cages

    Design, Structure, and Applications

    Edición de Takafumi Ueno, Sierin Lim, Kelin Xia ...
    Series series Springer Protocols
    This volume provides the latest methods for synthesis, structural analysis, and elucidation of the mechanism. Chapters guide readers through methods on protein cages for nanotechnology, analyze designed protein cages, determine the structures, and even perform theoretical analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an ... Leer más

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  • Plant Proteostasis

    Methods and Protocols

    Edición de L. Maria Lois, Marco Trujillo ...
    Series series Springer Protocols
    This second edition compiles state-of-art protocols to study the molecular mechanisms that determine proteome composition and contribute to maintaining protein homeostasis. Chapters describe methods that range from the in vitro analysis of enzyme cascades to real-time in vivo studies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their ... Leer más

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  • Advanced Methods in Structural Biology

    Edición de Ângela Sousa, Luis Passarinha ...
    Series series Springer Protocols
    This volume explores the latest integrated bioprocesses and technologies used to study the production of the target recombinant protein of therapeutic or diagnostic interest; its isolation, purification, and stabilization; and the bio-interaction and structural analyses. The chapters in this book are organized into four parts. Part One covers production methods of soluble and membrane proteins in ... Leer más

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