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DNA-Protein Interactions

Methods and Protocols

Edición de Marcos Simoes-Costa ...

Series series Springer Protocols
This volume details protocols emphasizing systems-level approaches that can be applied to genomic analyses. Chapters detail techniques for optimized application in in vivo systems, spatial, physiological, environmental contexts, imaging-based techniques, single-molecule approaches, CRISPR systems, new genomic approaches, and measurements of kinetics governing. Written in the format of the highly ... Leer más

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This volume details protocols emphasizing systems-level approaches that can be applied to genomic analyses. Chapters detail techniques for optimized application in in vivo systems, spatial, physiological, environmental contexts, imaging-based techniques, single-molecule approaches, CRISPR systems, new genomic approaches, and measurements of kinetics governing. Written in the format of the highly
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Bioinformatics and Functional Genomics

de Jonathan Pevsner ...

The bestselling introduction to bioinformatics and genomics – now in its third editionWidely received in its previous editions, Bioinformatics and Functional Genomics offers the most broad-based introduction to this explosive new discipline. Now in a thoroughly updated and expanded third edition, it continues to be the go-to source for students and professionals involved in biomedical research ... Leer más

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The bestselling introduction to bioinformatics and genomics – now in its third editionWidely received in its previous editions, Bioinformatics and Functional Genomics offers the most broad-based introduction to this explosive new discipline. Now in a thoroughly updated and expanded third edition, it continues to be the go-to source for students and professionals involved in biomedical research
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Synthetic Biology: A Lab Manual

de Josefine Liljeruhm, Erik Gullberg, Anthony C Forster ...

Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial ... Leer más

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Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial
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PCR Guru

An Ultimate Benchtop Reference for Molecular Biologists

de Ayaz Najafov, Gerta Hoxhaj ...

PCR Guru: An Ultimate Benchtop Reference for Molecular Biologists is provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with PCR setup and optimization. As a laboratory guide, it emphasizes the technical aspects of employing PCR as a tool in molecular biology laboratories. The book covers the history of PCR and the basic ... Leer más

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PCR Guru: An Ultimate Benchtop Reference for Molecular Biologists is provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with PCR setup and optimization. As a laboratory guide, it emphasizes the technical aspects of employing PCR as a tool in molecular biology laboratories. The book covers the history of PCR and the basic
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Drosophila

Methods and Protocols

Edición de Christian Dahmann ...

Series series Springer Protocols
This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and ... Leer más

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This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and
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Understanding PCR

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Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips. - Includes resources for ... Leer más

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Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips. - Includes resources for
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Catharanthus roseus

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Edición de Vincent Courdavault, Sebastien Besseau ...

Series series Springer Protocols
This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in C. roseus from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, transcription factor characterization up to functional genomic tools based on gene overexpression. Written in the format of the highly successful Methods in ... Leer más

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This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in C. roseus from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, transcription factor characterization up to functional genomic tools based on gene overexpression. Written in the format of the highly successful Methods in
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Series series Biomedical and Life Sciences (R0)
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This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a
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Edición de Thomas J. Nicholls, Jay P. Uhler, Maria Falkenberg ...

Series series Springer Protocols
This volume compiles a comprehensive range of methods to study key aspects of mitochondrial DNA including nucleoid structure and packaging, replication, genome integrity, and disease. Chapters are organized into eight methodological sections that cover in vitro and in vivo methods, including for mtDNA isolation, visualization, deep sequencing, gene editing, and diagnostic aspects of mtDNA disease. ... Leer más

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This volume compiles a comprehensive range of methods to study key aspects of mitochondrial DNA including nucleoid structure and packaging, replication, genome integrity, and disease. Chapters are organized into eight methodological sections that cover in vitro and in vivo methods, including for mtDNA isolation, visualization, deep sequencing, gene editing, and diagnostic aspects of mtDNA disease.
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Spatial Genome Organization

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Edición de Tom Sexton ...

Series series Springer Protocols
This detailed volume explores a variety of cutting-edge techniques used to interrogate spatial genome organization. Beginning with a section covering the vital chromosome conformation capture (3C) technique, this collection continues with chapters on targeted Hi-C approaches, sequencing-based approaches to assess nuclear environment, as well as single-cell technologies to better characterize the ... Leer más

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This detailed volume explores a variety of cutting-edge techniques used to interrogate spatial genome organization. Beginning with a section covering the vital chromosome conformation capture (3C) technique, this collection continues with chapters on targeted Hi-C approaches, sequencing-based approaches to assess nuclear environment, as well as single-cell technologies to better characterize the
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R-Loops

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Series series Springer Protocols
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This detailed book compiles a series of laboratory protocols covering the most important aspects of R-loop biology. Beginning with a range of methods allowing for the detection of DNA-RNA hybrids, as well as their purification and visualization by electron microscopy, the volume continues with methods based on the use of RNase H-derived tools to detect DNA-RNA hybrids in vitro and in vivo. Several
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Bioinformatics and Functional Genomics

Synthetic Biology: A Lab Manual

PCR Guru

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Understanding PCR

Catharanthus roseus

The Design and Development of Novel Drugs and Vaccines

A Practical Guide to Protein Engineering

Mitochondrial DNA

Spatial Genome Organization

R-Loops