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Genetically Incorporated Non-Canonical Amino Acids

Methods and Protocols

Edición de Yu-Hsuan Tsai, Simon J. Elsässer ...

Series series Springer Protocols
This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification. ... Leer más

$2,391 MXN

This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification.
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Phage Display of Peptides and Proteins

A Laboratory Manual

Edición de Brian K. Kay, Jill Winter, John McCafferty ...

Both novices and experts will benefit from this insightful step-by-step discussion of phage display protocols.Phage Display of Peptides and Proteins: A Laboratory Manual reviews the literature and outlines the strategies for maximizing the successful application of phage display technology to one's research. It contains the most up-to-date protocols for preparing peptide affinity reagents, ... Leer más

$730 MXN

Both novices and experts will benefit from this insightful step-by-step discussion of phage display protocols.Phage Display of Peptides and Proteins: A Laboratory Manual reviews the literature and outlines the strategies for maximizing the successful application of phage display technology to one's research. It contains the most up-to-date protocols for preparing peptide affinity reagents,
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Textbook Of Structural Biology

de Anders Liljas, Lars Liljas, Poul Nissen, Jure Piskur, Goran Lindblom, Morten Kjeldgaard ...

Latest Edition: Textbook of Structural Biology (2nd Edition)This is an important textbook for undergraduate and graduate students in structural biology, chemistry, biochemistry, biology and medicine. Written by a team of leading scientists in the field, it covers all the essential aspects of proteins, nucleic acids and lipids, including the rise and fall of proteins, membranes and gradients, the ... Leer más

$1,084 MXN

Latest Edition: Textbook of Structural Biology (2nd Edition)This is an important textbook for undergraduate and graduate students in structural biology, chemistry, biochemistry, biology and medicine. Written by a team of leading scientists in the field, it covers all the essential aspects of proteins, nucleic acids and lipids, including the rise and fall of proteins, membranes and gradients, the
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RNA Methodologies

Laboratory Guide for Isolation and Characterization

de Robert E. Farrell Jr. ...

This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of ... Leer más

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This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of
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Enzymatic Analysis

A Practical Guide

de Janet V. Passonneau, Oliver H. Lowry ...

Series series Biological Methods
Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refinement of old ones, and to the adaptation in general of methods to almost any scale of sensitivity. ... Leer más

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Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refinement of old ones, and to the adaptation in general of methods to almost any scale of sensitivity.
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Antibody Engineering Volume 1

Edición de Roland E. Kontermann, Stefan Dübel ...

Series series Springer Protocols
Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new ... Leer más

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Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new
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Alternative pre-mRNA Splicing

Theory and Protocols

Edición de Stefan Stamm, Chris Smith, Reinhard Lührmann ...

This book was written for graduate and medical students, as well as clinicians and postdoctoral researchers. It describes the theory of alternative pre-mRNA splicing intwelve introductory chapters and then introduces protocols and their theoretical background relevant for experimental research. These 43 practical chapters cover: Basic methods, Detection of splicing events, Analysis of alternative ... Leer más

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This book was written for graduate and medical students, as well as clinicians and postdoctoral researchers. It describes the theory of alternative pre-mRNA splicing intwelve introductory chapters and then introduces protocols and their theoretical background relevant for experimental research. These 43 practical chapters cover: Basic methods, Detection of splicing events, Analysis of alternative
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RNA-Protein Complexes and Interactions

Methods and Protocols

Edición de Ren-Jang Lin ...

Series series Springer Protocols
This second edition updates, complements, and expands upon the first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. Chapters explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. Written in ... Leer más

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This second edition updates, complements, and expands upon the first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. Chapters explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. Written in
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Protein Arginylation

Methods and Protocols

Edición de Anna S. Kashina ...

Series series Springer Protocols
This second edition provides updated and new chapters that detail comprehensive overview of all the existing methods on analyzing protein arginylation. Chapters guide readers through the early methods utilizing crude protein preparations and whole-cell assays to the latest advanced methods involving recombinant protein techniques, antibodies, high precision mass spectrometry, and chemical probes. ... Leer más

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This second edition provides updated and new chapters that detail comprehensive overview of all the existing methods on analyzing protein arginylation. Chapters guide readers through the early methods utilizing crude protein preparations and whole-cell assays to the latest advanced methods involving recombinant protein techniques, antibodies, high precision mass spectrometry, and chemical probes.
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The Mitoribosome

Methods and Protocols

Edición de Antoni Barrientos, Flavia Fontanesi ...

Series series Springer Protocols
This volume provides classic and new methods to study the structure, assembly pathway, and protein synthesis ability of mitoribosomes across species. Following an introduction of fundamental concepts on the topic, method chapters present detailed protocols based on cryo-electron tomography, cryo-EM approaches, mitoribosome purification techniques, mitochondrial translation assays, and methods to ... Leer más

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This volume provides classic and new methods to study the structure, assembly pathway, and protein synthesis ability of mitoribosomes across species. Following an introduction of fundamental concepts on the topic, method chapters present detailed protocols based on cryo-electron tomography, cryo-EM approaches, mitoribosome purification techniques, mitochondrial translation assays, and methods to
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Mitochondrial Medicine

Volume 2: Assessing Mitochondria

Edición de Volkmar Weissig, Marvin Edeas ...

Series series Springer Protocols
This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume II guides readers through chapters on mitochondrial dysfunction, functional’ mitochondria, mitochondrial retrograde, mitochondrial dNTP pool quantification, mitochondrial ADP-ribosylation, blue-native gel approach, 3D ... Leer más

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This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume II guides readers through chapters on mitochondrial dysfunction, functional’ mitochondria, mitochondrial retrograde, mitochondrial dNTP pool quantification, mitochondrial ADP-ribosylation, blue-native gel approach, 3D
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Chemical Protein Synthesis

Edición de Xuechen Li ...

Series series Springer Protocols
This volume provides updated protocols for chemical protein synthesis. Chapters guide readers through development methods, strategies, and applications of protein chemical synthesis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and ... Leer más

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This volume provides updated protocols for chemical protein synthesis. Chapters guide readers through development methods, strategies, and applications of protein chemical synthesis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and
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Phage Display of Peptides and Proteins

Textbook Of Structural Biology

RNA Methodologies

Enzymatic Analysis

Antibody Engineering Volume 1

Alternative pre-mRNA Splicing

RNA-Protein Complexes and Interactions

Protein Arginylation

The Mitoribosome

Mitochondrial Medicine

Chemical Protein Synthesis