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    Series series Springer Protocols
    This fully updated volume presents a diverse collection of protocols and methodologies for studying various molecular motors. The book explores the dynamics of the bacterial flagellar motor, the mechanisms of ATP synthase, the assembly and function of cytoskeletal motors, and the role of nucleic acid motors in cellular processes, while placing a strong emphasis on state-of-the-art techniques, ... Leer más

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  • The Nuclear Membrane

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    Edición de Yuki Hara, Kazunori Kume ...
    Series series Springer Protocols
    This book compiles protocols that encompass a diverse range of classical and contemporary approaches, including biochemical techniques, proteomic analyses, microscopic observations, and biophysical measurements. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions tothe material, lists of necessary materials and reagents, step-by ... Leer más

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  • Iron Metabolism

    Methods and Protocols

    Edición de Oleh Khalimonchuk ...
    Series series Springer Protocols
    This detailed volume explores classical and cutting-edge methods optimized and validated to analyze various aspects of iron metabolism, from in vitro to multi-organ level. Opening with a section on basic iron metabolism methods, the book continues with methods applicable to a variety of systems, ranging from bacteria to cultured mammalian cells and tissues, with a focus on cellular heme and iron ... Leer más

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  • The Mitotic Spindle

    Methods and Protocols

    Edición de Tomomi Kiyomitsu ...
    Series series Springer Protocols
    This detailed volume presents methods for investigating mitotic spindle assembly, chromosome segregation, and cytokinesis. Split into six parts, the book examines advanced microscopes, spatiotemporal manipulation of the spindle and target molecules, quantitative live imaging, screening, centrosome manipulation and purification, and spindle positioning in synthetic or complicated contexts. Written ... Leer más

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  • RNA Amplification and Analysis

    Methods and Protocols

    Edición de Mekbib Astatke ...
    Series series Springer Protocols
    This volume provides methods to analyze RNA and gain insight to a number of critical cellular functions. Chapters detail RNA isolation and enrichment, RNA amplification and detection, RNA sequencing, analysis of RNA modification, RNA delivery approaches, and analysis of RNA binding and catalytic activity. Written in the highly successful Methods in Molecular Biology series format, chapters include ... Leer más

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  • Bacterial Chromatin

    Methods and Protocols

    Edición de Remus T. Dame ...
    Series series Springer Protocols
    This second edition brings together new and updated methods to explore the structure and function of bacterial chromatin from molecular to the cellular scale. Chapters detail experimental protocols of in vivo and in vitro approaches, approaches to genome structure modeling, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include ... Leer más

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  • DNA Nanotechnology

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    Edición de Giampaolo Zuccheri ...
    Series series Springer Protocols
    This fully updated volume explores methods to create and use nanostructures for characterizing, targeting, and controlling the behavior of cells. Experts from around the globe present their procedures for designing, preparing, characterizing, and using DNA nanostructures, especially towards biological or materials science applications. Additionally, the study of fundamental aspects of nucleic ... Leer más

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  • Photorespiration

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    Edición de Berkley J Walker ...
    Series series Springer Protocols
    This volume provides methods on researching photorespiration to provide a better understanding of this vital pathway in plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting ... Leer más

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    Edición de Andrzej Stanisław Cieplak ...
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    The volume details techniques, methods, and conceptual developments to further the study of protein aggregation with emphasis on the pleiomorphic proteins implicated in etiology of neurodegeneration. Chapters guide readers through in vitro and in vivo studies of fibrillization and liquid-liquid phase separation processes, and offer a comprehensive account of the state-of-art of structural studies ... Leer más

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  • Fluorescent Proteins

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    Edición de Mayank Sharma ...
    Series series Springer Protocols
    This volume brings together cutting-edge laboratory protocols to characterize the novel fluorescent proteins (FPs) and approaches based on fluorescent proteins that aim to answer some of the key cell biological questions. The book covers topics ranging from the database of fluorescent proteins to their characterization and adaptation to a wide range of biological systems. Written for the highly ... Leer más

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  • Mitotic Exit

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    Edición de Victor M. Bolanos-Garcia ...
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    This volume explores the latest approaches and techniques used to study mitotic exit in diverse model organisms. The chapters in this book cover topics such as transgenic methods generation of RNAi-sensitive cell lines; gene overexpression in heterologue gene expression systems; quantitative live cell imaging and FRET-FLIM; biochemical protocols for analyzing post-translational modifications ... Leer más

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