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  • SNAREs

    Methods and Protocols

    Edición de Rutilio Fratti ...
    Series series Springer Protocols
    This second edition details new and updated methods on soluble N-ethylmaleimide-sensitive factor attachment protein receptors( SNAREs) and their function are examined in the laboratory. Chapters guide readers through an overview of the basic properties of SNAREs, distribution and interaction with regulators of membrane fusion, activation of SNAREs in the priming stage by NSF/Sec18 and a-SNAP/Sec17 ... Leer más

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  • Mass Spectrometry-Based Proteomics

    Edición de Kris Gevaert ...
    Series series Springer Protocols
    This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by ... Leer más

    $3,095 MXN

  • Golgi

    Methods and Protocols

    Series series Springer Protocols
    This volume provides readers with a collection of new and classical methods, techniques, and applications used to address enduring questions about the structure and functions of the Golgi complex. The chapters in this volume cover diverse topics ranging from model systems; live and fixed cell imaging techniques; in vitro biochemical reconstitution systems; and specific methods developed to study ... Leer más

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  • Methods for Plant Nucleus and Chromatin Studies

    Methods and Protocols

    Edición de Célia Baroux, Christophe Tatout ...
    Series series Springer Protocols
    This volume provides a comprehensive collection of protocols that can be used to study plant chromatin structure and composition. Chapters divided into five sections detail the profiling of chromatin features in relation to epigenetic regulation, investigate the interaction between chromatin modifications and gene regulation, and explore the 3D spatial organization of the chromatin inside the ... Leer más

    $4,213 MXN

  • Bacterial Regulatory RNA

    Methods and Protocols

    Series series Springer Protocols
    This second edition details new and updated methods used for studying prokaryotic non-coding RNAs and their protein accomplices. Chapters detail discovery of ncRNAs, characterization of their structure, functions, and their interactomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary ... Leer más

    $2,407 MXN

  • Single Cell ‘Omics of Neuronal Cells

    Series series Springer Protocols
    This volume discusses the latest techniques used in the diverse fields of single cell ‘omics and covers topics such as quantifying the single cell transcriptome; isolation of cells in nanoliter volumes for single cell proteomics measurements by nano-LC-MS/MS; and single cell protein characterization by immunoblotting. A wide range of methodologies are highlighted, ranging from high-yield chemical ... Leer más

    $2,759 MXN

  • Protein Aggregation

    Methods and Protocols

    Edición de Andrzej Stanisław Cieplak ...
    Series series Springer Protocols
    The volume details techniques, methods, and conceptual developments to further the study of protein aggregation with emphasis on the pleiomorphic proteins implicated in etiology of neurodegeneration. Chapters guide readers through in vitro and in vivo studies of fibrillization and liquid-liquid phase separation processes, and offer a comprehensive account of the state-of-art of structural studies ... Leer más

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  • piRNA

    Methods and Protocols

    Series series Springer Protocols
    This detailed volume explores newly-developed methods in PIWI-interacting RNAs (piRNAs) research, methods currently applied to other ncRNAs involved in nuclear regulation which can be used to study piRNAs, and piRNA methods applied in non-classical organisms. It also includes several bioinformatic and biophysical methods related to piRNA studies, consistent with the increasing importance of high ... Leer más

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  • Bacterial Secretion Systems

    Methods and Protocols

    Edición de Laure Journet, Eric Cascales ...
    Series series Springer Protocols
    This second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or ... Leer más

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  • Dictyostelium discoideum

    Methods and Protocols

    Edición de Alan R. Kimmel ...
    Series series Springer Protocols
    This volume discusses the latest advancements and techniques used to study Dictyostelium discoideum. The chapters in this book cover topics such as cytokinesis, membrane function and damage, and nutrient/bacterial processing; autophagy activation and function; protein localization, interaction, and activation; cell migration and contact guidance for quantification of cytoskeletal dynamics; and ... Leer más

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  • Structural Genomics and Drug Discovery

    Methods and Protocols

    Edición de Wayne F. Anderson ...
    Series series Springer Protocols
    Structural Genomics and Drug Discovery: Methods and Protocols focuses on high throughput structure determination methods and how they can be applied to lay the groundwork for structure aided drug discovery. The methods and protocols that are described can be applied in any laboratory interested in using detailed structural information to advance the initial stages of drug discovery. Written in the ... Leer más

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  • The Immune Synapse

    Methods and Protocols

    Series series Springer Protocols
    This new collection features the most up-to-date essential protocols that are currently being used to study the immune synapse. Beginning with methods for making biophysical measurements, the volume continues by covering the cell biology of synapses, methods for advanced substrate engineering, mechanobiology topics, new technologies to describe and manipulate synaptic components, as well as ... Leer más

    $3,311 MXN