As part of a collaboration between two different groups in chemistry and biochemistry, Thom Sharp presents here his thesis work on the development of new methods for cryoelectron microscopy. Throughout his Ph.D., Thom had to master a whole range of techniques including modelling, molecular biology and microscopy. Using these skills to tackle an outstanding problem, the pursuit of high-resolution structures of peptide-based materials, Thom highlights in this thesis his newly developed metho...

Addressing the widespread need for a practical guide to imaging mass spectrometry (IMS), this book presents the protocols of IMS technology. As that technology expands, research groups around the world continue its development. Pharmaceutical companies are using IMS for drug analyses to study pharmacokinetics and medical properties of drugs. Drug research and disease-related biomarker screening are experiencing greater use of this technology, with a concurrent increase in the number of res...

This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors.The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in ...

This thoroughly revised and updated Fourth Edition of a time-honored text provides the reader with a comprehensive introduction to the field of scanning electron microscopy (SEM), energy dispersive X-ray spectrometry (EDS) for elemental microanalysis, electron backscatter diffraction analysis (EBSD) for micro-crystallography, and focused ion beams. Students and academic researchers will find the text to be an authoritative and scholarly resource, while SEM operators and a diversity of prac...

The 2nd International Multidisciplinary Microscopy and Microanalysis Congress & Exhibition (InterM 2014) was held on 16–19 October 2014 in Oludeniz, Fethiye/ Mugla, Turkey.The aim of the congress was to gather scientists from various branches and discuss the latest improvements in the field of microscopy. The focus of the congress has been widened in an "interdisciplinary" manner, so as to allow all scientists working on several related subjects to participate and present their wor...

Reviews in Fluorescence 2009, the sixth volume of the book serial from Springer, serves as a comprehensive collection of current trends and emerging hot topics in the field of fluorescence and closely related disciplines. It summarizes the year’s progress in fluorescence and its applications, with authoritative analytical reviews specialized enough to be attractive to professional researchers, yet also appealing to the wider audience of scientists in related disciplines of fluorescence...

Although the official compendia define a drug substance as to identity, purity, strength, and quality, they normally do not provide other physical or chemical data, nor do they list methods of synthesis or pathways of physical or biological degradation and metabolism. Such information is scattered throughout the scientific literature and the files of pharmaceutical laboratories. Edited by the Associate Director of Analytical Research and Development for the American Association of Pharmace...

This book presents methodological and application research in detecting cellular and molecular biophysical properties based on atomic force microscopy (AFM) nanorobotics. Series methods for in situ label-free visualizing and quantifying the multiple physical properties of single cells and single molecules were developed, including immobilization strategies for observing fine structures of living cells, measurements of single-cell mechanics, force recognition of molecular interactions, and ...

This book highlights the various topics in which luminescence and electrochemistry are intimately coupled. The topic of this book is clearly at the frontier between several scientific domains involving physics, chemistry and biology. Applications in these various fields naturally also need to be mentioned, especially concerning displays and advanced investigation techniques in analytical chemistry or for biomedical issues.

This book is the autobiography of Shinya Inoué, tracing his life from childhood to the present. Though he has made many contributions to science, perhaps the most remarkable one involves the visualization of dynamics in living cells by means of a polarizing light microscope, an innovation that changed the face of cell biology.Addressing readers curious to know why and how he achieved such success, the story begins with a prologue describing the end of World War II and Inoué’s lifel...

Dr. Chris D. Geddes, Ph.D., Professor, has extensive experience in fluorescence spectroscopy, particularly in fluorescence sensing and metal-fluorophore interactions (Metal-Enhanced Fluorescence), publishing over 190 papers and 18 books. Dr. Geddes is internationally known in fluorescence. He is the editor-in-chief of the Journal of Fluorescence and founding editor of the Who’s Who in Fluorescence and Annual Reviews in Fluorescence volumes. In addition, due to th...

This thesis describes novel approaches and implementation of high-resolution microscopy in the extreme ultraviolet light regime. Using coherent ultrafast laser-generated short wavelength radiation for illuminating samples allows imaging beyond the resolution of visible-light microscopes. Michael Zürch gives a comprehensive overview of the fundamentals and techniques involved, starting from the laser-based frequency conversion scheme and its technical implementation as well as general consi...