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Mouse Embryogenesis

Methods and Protocols

Editat de Paul Delgado Olguin ...

Seria seria Springer Protocols
This second edition volume expands on the previous edition with new methods and refinements ranging from tissue clearing and three-dimensional imaging, to explant culture systems, lineage tracing, high-resolution transcriptomics, metabolism, and chromatin structure analysis. The chapters in this book are organized by molecular depth, progressing from foundational methods to approaches that probe ... Citiți mai mult

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This second edition volume expands on the previous edition with new methods and refinements ranging from tissue clearing and three-dimensional imaging, to explant culture systems, lineage tracing, high-resolution transcriptomics, metabolism, and chromatin structure analysis. The chapters in this book are organized by molecular depth, progressing from foundational methods to approaches that probe
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The AGT Cytogenetics Laboratory Manual

Editat de Marilyn S. Arsham, Margaret J. Barch, Helen J. Lawce ...

Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics.Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis ... Citiți mai mult

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Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics.Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis
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Virus-Host Interactions

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Editat de Marilena Aquino de Muro ...

Seria seria Springer Protocols
This detailed volume spotlights methods to investigate a variety of virus-host interactions in humans, other mammals, fish, or insects. It explores viruses such as white spot syndrome virus (WSSV), honeybee viruses, Nipah virus, EBV, SVCV, HSV-1, HIV-1, A H1N1, and SARS-CoV-2, as well as applications of techniques such as qPCR, serum antibody responses, 4C analysis, cell membrane fusion, ... Citiți mai mult

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This detailed volume spotlights methods to investigate a variety of virus-host interactions in humans, other mammals, fish, or insects. It explores viruses such as white spot syndrome virus (WSSV), honeybee viruses, Nipah virus, EBV, SVCV, HSV-1, HIV-1, A H1N1, and SARS-CoV-2, as well as applications of techniques such as qPCR, serum antibody responses, 4C analysis, cell membrane fusion,
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Stem Cells and Aging

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Editat de Kursad Turksen ...

Seria seria Springer Protocols
This fully updated book collects protocols that reflect current investigations into how stem cell populations change during organismal aging. These methodologies are well-established and described in easy-to-follow fashion so as to be valuable for not only experts but also novices in this ever-developing area of stem cell research. Written for the highly successful Methods in Molecular Biology ... Citiți mai mult

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This fully updated book collects protocols that reflect current investigations into how stem cell populations change during organismal aging. These methodologies are well-established and described in easy-to-follow fashion so as to be valuable for not only experts but also novices in this ever-developing area of stem cell research. Written for the highly successful Methods in Molecular Biology
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Bacteriophage Therapy

From Lab to Clinical Practice

Editat de Joana Azeredo, Sanna Sillankorva ...

Seria seria Springer Protocols
This highly anticipated second edition details cutting-edge experimental approaches for bacteriophage research. This comprehensive volume details new and updated experimental approaches for isolating, formulating, and engineering bacteriophage medicinal products. Authored by leading experts and written in the highly successful Methods in Molecular Biology series format, each chapter includes ... Citiți mai mult

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This highly anticipated second edition details cutting-edge experimental approaches for bacteriophage research. This comprehensive volume details new and updated experimental approaches for isolating, formulating, and engineering bacteriophage medicinal products. Authored by leading experts and written in the highly successful Methods in Molecular Biology series format, each chapter includes
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Vaccine Design

Methods and Protocols, Volume 1. Vaccines for Human Diseases

Editat de Sunil Thomas ...

Seria seria Springer Protocols
This volume provides a practical guide providing step-by-step protocol to design and develop vaccines for human diseases. Divided into three volumes, Volume 1: Vaccines for Human Diseases guides readers through an introductory section on future challenges for vaccinologists and the immunological mechanism of vaccines. Chapters focus on design of human vaccines for viral, bacterial, fungal, and ... Citiți mai mult

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This volume provides a practical guide providing step-by-step protocol to design and develop vaccines for human diseases. Divided into three volumes, Volume 1: Vaccines for Human Diseases guides readers through an introductory section on future challenges for vaccinologists and the immunological mechanism of vaccines. Chapters focus on design of human vaccines for viral, bacterial, fungal, and
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Hepatocytes

Methods and Protocols

Editat de Naoki Tanimizu ...

Seria seria Springer Protocols
This volume details experimental protocols to study structure and function of hepatocytes in vivo and in vitro, and for characterizing heterogeneity and plasticity of hepatocytes in healthy and in injured liver. Chapters guide readers through separating hepatocyte subfractions, characterizing the heterogeneity, novel culture systems, hepatocyte-like cells from human pluripotent stem cells, ... Citiți mai mult

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This volume details experimental protocols to study structure and function of hepatocytes in vivo and in vitro, and for characterizing heterogeneity and plasticity of hepatocytes in healthy and in injured liver. Chapters guide readers through separating hepatocyte subfractions, characterizing the heterogeneity, novel culture systems, hepatocyte-like cells from human pluripotent stem cells,
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Induced Pluripotent Stem Cells and Human Disease

Methods and Protocols

Editat de Kursad Turksen ...

Seria seria Springer Protocols
This detailed volume presents a series of protocols that are representative of recent developments and improvements in induced pluripotent stem cells (iPS cells) and corresponding human disease models. Reflecting the latest technology for generating induced pluripotent stem cells (iPS cells) and their initial characterization, the book explores techniques invaluable both for studies of disease ... Citiți mai mult

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This detailed volume presents a series of protocols that are representative of recent developments and improvements in induced pluripotent stem cells (iPS cells) and corresponding human disease models. Reflecting the latest technology for generating induced pluripotent stem cells (iPS cells) and their initial characterization, the book explores techniques invaluable both for studies of disease
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Ocular Regeneration

Methods and Protocols

Editat de Jeffrey M. Gross ...

Seria seria Springer Protocols
This detailed volume presents state-of-the-art techniques in ocular regeneration research, pulling together recent animal model systems identifying key genes and regulatory networks that contribute to the regenerative response as well as advances in induced pluripotent stem cell (iPSC) biology. The book opens with a section on the anterior segment and key models and techniques utilized to study ... Citiți mai mult

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This detailed volume presents state-of-the-art techniques in ocular regeneration research, pulling together recent animal model systems identifying key genes and regulatory networks that contribute to the regenerative response as well as advances in induced pluripotent stem cell (iPSC) biology. The book opens with a section on the anterior segment and key models and techniques utilized to study
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Induced Pluripotent Stem (iPS) Cells

Methods and Protocols

Editat de Andras Nagy, Kursad Turksen ...

Seria seria Springer Protocols
This extensive new edition presents protocols reflecting the great strides made in the study of induced pluripotent stem (iPS) cells. The collection explores new and improved methods for the generation, expansion, and maintenance of iPS cells from different tissue types, characterization of their differentiation pathways along different lineages, and their potential utility in tissue repair and ... Citiți mai mult

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This extensive new edition presents protocols reflecting the great strides made in the study of induced pluripotent stem (iPS) cells. The collection explores new and improved methods for the generation, expansion, and maintenance of iPS cells from different tissue types, characterization of their differentiation pathways along different lineages, and their potential utility in tissue repair and
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Tissue-Resident Macrophages

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Editat de Elvira Mass ...

Seria seria Springer Protocols
This detailed book delves into the diverse techniques and applications to target, isolate, image, phenotype, and analyze tissue-resident and monocyte-derived macrophages. The contents aim to describe the current knowledge about macrophage development and function which forces the scientific field to move beyond the previously described M1/M2 macrophage paradigm to be able to dissect macrophage ... Citiți mai mult

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This detailed book delves into the diverse techniques and applications to target, isolate, image, phenotype, and analyze tissue-resident and monocyte-derived macrophages. The contents aim to describe the current knowledge about macrophage development and function which forces the scientific field to move beyond the previously described M1/M2 macrophage paradigm to be able to dissect macrophage
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Pseudomonas aeruginosa

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Editat de Giovanni Bertoni, Silvia Ferrara ...

Seria seria Springer Protocols
This book aims to provide methods, protocols, and discussion topics for those who wish to examine in depth the molecular mechanisms of adaptation and versality of bacteria and would like to envisage their evolution responses in the fast changing Antropocene.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists ... Citiți mai mult

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This book aims to provide methods, protocols, and discussion topics for those who wish to examine in depth the molecular mechanisms of adaptation and versality of bacteria and would like to envisage their evolution responses in the fast changing Antropocene.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists
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Springer Protocols Serie de Cărți electronice

Mouse Embryogenesis

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Tissue-Resident Macrophages

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