The second volume of The Expanding Continuum of Gender-Based Violence: Trauma-Informed Care examines the impact of violence on contemporary populations which may previously have been overlooked. In this volume, special focus is given to the intersectionality of vulnerable groups and the impact of marginalization on assessment and treatment. In an effort to bridge gaps in literature, the text addresses cultural influence and socio-political issues while advising on how to best apply the acc...

A unique love story evolves from an encounter between two souls in the margins of the temporal and infinite worlds. While driving across country in 1983, Michael Stone has an opportunity to visit the small town of his childhood. Upon arrival, he finds himself some 40 years in the past. Unnerved in his strange new circumstance, his mutual attraction with this fascinating young woman named Virginia Shelby is his only stability. Mike gradually embraces his new life and shares a profound love ...

The first volume of The Expanding Continuum of Gender-Based Violence: Trauma-Informed Care is a thorough examination of current knowledge on the way gender impacts both group and individual risk of violence. In this volume, interdisciplinary professionals focus on the presentation and assessment of trauma with a special focus on trauma-informed approaches. Special emphasis is placed on inclusive language recommendations in order to guide clinicians and care providers in current best practi...

The first volume of Mental Health Issues of Child Maltreatment: Contemporary Strategies is a thorough examination of current knowledge on the way child abuse and neglect can affect a child’s mental and emotional development. In this volume interdisciplinary professionals focus on the presentation and assessment of trauma with a special focus on post-pandemic emerging trends in diagnosis. This text intends to do the following: — Define the problem of child maltreatment conceptually and nume...

The second volume of Mental Health Issues of Child Maltreatment: Contemporary Strategies offers a well-rounded and comprehensive perspective on topics related to child abuse and neglect, including a focused look at intersectional populations at risk of maltreatment and how best to provide trauma-informed care. This text intends to do the following: —Define the problem of child maltreatment conceptually and numerically, using statistics that describe the state of maltreatment and the charac...

Advanced Methodologies to Study and Enhance Cellular Cytotoxicity Against Cancer, Volume 207 covers the latest techniques and strategies used to investigate and boost cytotoxic activity in cells targeting cancer. Chapters include An ex vivo assay to assess CAR T cell cytotoxicity in viable tumor tissues using a two-step staining protocol, New protocol to measure mitochondria polarization/function at the immunological synapse, Analysis of CAR-T cell–mediated cytotoxicity in solid tumor mode...

The Immunological Synapse - Part C, Volume 193 in the Methods in Cell Biology series provides state-of-the-art and detailed methods for the study of the T cell, natural killer (NK) cell and B cell immunological synapses. Topics covered in this third volume include tau-STED microscopy for 3D-quantitative colocalization of lytic granule markers, image processing approaches for 3D-analysis of markers and for microtubule remodeling quantification at the immunological synapse, flow cytometry to...

The Immunological Synapse - Part B, Volume 178 in the Methods in Cell Biology series provides state-of-the-art methods for the study of the immunological synapse. This first volume covers various aspects on T cell and natural killer (NK) cell synapses, including imaging polarized granule release using TIRF microscopy, analysis of actin reorganization and centrosome polarization, redirected degranulation, live cell-imaging to quantify cytotoxic and chemotactic dynamics, quantification of in...

The Immunological Synapse, Part A, Volume 173 in the Methods in Cell Biology series provides state-of-the-art methods for the study of the immunological synapse. Sections cover Imaging polarized granule release at the cytotoxic T cell immunological synapse using TIRF microscopy: control by polarity regulators, Analysis of centrosomal area actin reorganization and centrosome polarization upon lymphocyte activation at the immunological synapse, P815-based redirected degranulation assay to st...

Cancer cell biology research in general, and anti-cancer drug development specifically, still relies on standard cell culture techniques that place the cells in an unnatural environment. As a consequence, growing tumor cells in plastic dishes places a selective pressure that substantially alters their original molecular and phenotypic properties.The emerging field of regenerative medicine has developed bioengineered tissue platforms that can better mimic the structure and cellular heteroge...

G-Protein-Coupled Receptors, Part B, 2nd Edition, Volume 149, the latest release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers Optical Approaches for Visualization of Arrestin Binding to Muscarinic Receptors, Luciferase Reporter Assay for Unlocking Ligand-mediated Signaling of GPCRs, Assays to Measure GPCR Dependent Cellular Migration, Characterization of the Frizzled GPCRs, Bind...

This second edition volume brings together some of the best experts in the field of modern metabolomics to discuss the latest various techniques used to study specific metabolite classes, and metabolomics in bacterial and mammalian systems. The chapters in this book cover topics such as handling big data for metabolite identification and quantification, as well as building pathways for comparison with other omes, Isotopic Ratio Outlier Analysis (IROA) for quantitative analysis; cholesterol...