This detailed collection covers methods recently developed for experiments on organisms from each of the major euglenozoan groups, including diplonemids, euglenids, and kinetoplastids. Volume 2 presents three sections exploring techniques in molecular biology, cell biology, as well as biochemistry and physiology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and rea...

This detailed collection covers methods recently developed for experiments on organisms from each of the major euglenozoan groups, including diplonemids, euglenids, and kinetoplastids. Volume 1 presents a historical review of the research into euglenozoans, followed by sections of techniques involving isolation and culturing of different species of euglenozoans, as well as a variety of -omics research methods. Written for the highly successful Methods in Molecular Biology series, ...

This volume explores the latest methods used by researchers to study different trypanosomatid parasites. These methods cover numerous disciplines, from organismal biology to molecular mechanism. The chapters in this book cover topics such as high-throughput sequencing; next-generation analysis of trypanosomatid genome stability and instability; DNA repair in cell extracts; ribosome profiling; and the use of CRISPR/Cas9 technology for gene editing. Written in the highly successful Metho...

This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the vertebrate nervous system, tools and approaches using zebrafish to study stem cell and regenerative ...

This volume details protocols on meiosis, covering the latest technological and methodological advances in this research field. Chapters guide readers through methods on genomics, biochemistry, super-resolution microscopy, traditional genetics, cytological methods, as well as machine learning and in silico modelling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary ma...

This detailed volume examines fine-tuned methodologies using the planarian species, Schmidtea mediterranea. The book features experimental protocols covering topics from in situ hybridization, immunohistochemistry, cell dissociation and flow cytometry, to pipelines for the analysis of large datasets, as in genomics and transcriptomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of...

Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics.Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis of G-banded chromosomes, specialized stains that address specific chromosomal structur...

This fully updated volume explores improved and extended protocols for embryonic stem cell (ESC) research, provided with the most direct and informative methodologies currently available. The book examines how these models for cell lineage and differentiation studies have continued to mature into a critical research workhorse. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necess...

This fully updated volume assembles a comprehensive collection of methods, techniques, and strategies to investigate the molecular and cellular biology of peroxisomes in different organisms. Peroxisome research is on the rise, as novel functions and proteins of this dynamic organelle are still being discovered through studies in model systems including humans, mice, flies, plants, fungi, and yeast, and this progress is reflected in the chapters included in this collection. Written for the ...

Laboratory Protocols in Fungal Biology presents the latest techniques in fungal biology. This book analyzes information derived through real experiments, and focuses on cutting edge techniques in the field. The book comprises 57 chapters contributed from internationally recognised scientists and researchers. Experts in the field have provided up-to-date protocols covering a range of frequently used methods in fungal biology. Almost all important methods available in the area of fu...

This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and cell lineage tracing, protein inhibition and tagging, optogenetic and optochemical control of...

This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by proteases, protein N-glycosylation, and protein phosphorylation. The book also collects chapters o...