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  • Plant Iron Homeostasis

    Methods and Protocols

    Redigeret af Jeeyon Jeong ...
    Serier serie Springer Protocols
    This detailed volume focuses on iron homeostasis in plants, iron being an essential micronutrient that serves as a cofactor in numerous metabolic processes but is harmful in excess. Specifically, the content ranges from protocols to study the iron deficiency response, the interaction between root and microbes under iron deficient conditions, the transcriptional network of iron homeostasis, ... Læs mere

    1.867,83 kr.

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    Innovations and Future Directions

    Serier serie Advanced Biotechnology
    A comprehensive overview of the topic, highlighting recent developments, ongoing research trends and future directions.Experts from Europe, Asia and the US cover five core areas of imminent importance to the food, feed, pharmaceutical and water treatment industries in terms of sustainable and innovative processing and production. In the field of enzyme engineering, they summarize historic ... Læs mere

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  • Biotechnologies for Plant Mutation Breeding

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    This book is open access under a CC BY-NC 2.5 license.This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular ... Læs mere

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  • Catharanthus roseus

    Methods and Protocols

    Serier serie Springer Protocols
    This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in C. roseus from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, transcription factor characterization up to functional genomic tools based on gene overexpression. Written in the format of the highly successful Methods in ... Læs mere

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  • Plant Lipid Signaling Protocols

    Redigeret af Teun Munnik, Ingo Heilmann ...
    Serier serie Springer Protocols
    As scientist begin to understand the complexity of lipid signaling and its roles in plant biology, there is an increasing interest in their analysis. Due to the low abundancy and transient nature of some of these hydrophobic compounds, this is not always easy. In Plant Lipid Signaling Protocols, expert researchers in the field detail experimental approaches by which plant signaling lipids can be ... Læs mere

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  • Plant Proteostasis

    Methods and Protocols

    Redigeret af L. Maria Lois, Marco Trujillo ...
    Serier serie Springer Protocols
    This second edition compiles state-of-art protocols to study the molecular mechanisms that determine proteome composition and contribute to maintaining protein homeostasis. Chapters describe methods that range from the in vitro analysis of enzyme cascades to real-time in vivo studies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their ... Læs mere

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  • Engineered Living Materials

    Redigeret af Wil V. Srubar III ...
    Serier serie Biomedical and Life Sciences (R0)
    This book will serve as a primer for readers to understand recent advances, applications, and current challenges in the field of Engineered Living Materials. The chapters cover core science and engineering research areas, including (1) advances in synthetic biology and genetic programmability for Engineered Living Materials, (2) functional Engineered Living Material for application in energy, ... Læs mere

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  • Plant Abiotic Stress Signaling

    Redigeret af Ivan Couée ...
    Serier serie Springer Protocols
    This volume provides conceptual strategies and methodological know-how over a wide range of stress situations that can be used as stepping stones to unravel the intricacies of abiotic stress signaling networks in plants. Chapters guide readers through achievements and challenges in the field and through up-to-date protocols covering identification of novel processes, validation of hypothetical ... Læs mere

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  • Protein Chromatography

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    This third edition expands on the previous editions with updated and new chapters on protein chromatography. Chapters detail protein stability and storage, avoiding proteolysis, protein quantitation methods, generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins. Written in the format of the highly successful Methods in Molecular Biology ... Læs mere

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  • Chemical Protein Synthesis

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    This volume provides updated protocols for chemical protein synthesis. Chapters guide readers through development methods, strategies, and applications of protein chemical synthesis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and ... Læs mere

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  • Proximity Labeling

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    Redigeret af Murat Sunbul, Andres Jäschke ...
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    This book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived reactive species to non-specifically label molecules within a certain radius of up to twenty nanometers. Beginning with peroxidase-based proximity ... Læs mere

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  • Heterologous Expression of Membrane Proteins

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    This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus ... Læs mere

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