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Nutrient Sensing in Eukaryotes

Edición de Fiona M. Russell, D. Grahame Hardie ...

Series series Springer Protocols
This detailed volume explores various methods through which nutrient sensing can be studied, particularly in multicellular eukaryotes. Beginning with a section on the monitoring of cellular energy status by sensing of adenine nucleotides, the book continues by delving into numerous techniques for researching both intracellular and extracellular nutrient sensing. Written for the highly successful ... Leer más

$3,127 MXN

This detailed volume explores various methods through which nutrient sensing can be studied, particularly in multicellular eukaryotes. Beginning with a section on the monitoring of cellular energy status by sensing of adenine nucleotides, the book continues by delving into numerous techniques for researching both intracellular and extracellular nutrient sensing. Written for the highly successful
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Mass Spectrometry-Based Proteomics

Edición de Kris Gevaert ...

Series series Springer Protocols
This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by ... Leer más

$3,095 MXN

This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by
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Antibody Engineering Volume 1

Edición de Roland E. Kontermann, Stefan Dübel ...

Series series Springer Protocols
Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new ... Leer más

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Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new
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Applied Biocatalysis

The Chemist's Enzyme Toolbox

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Provides clear and comprehensive coverage of recently developed applied biocatalysis for synthetic organic chemists with an emphasis to promote green chemistry in pharmaceutical and process chemistryThis book aims to make biocatalysis more accessible to both academic and industrial synthetic organic chemists. It focuses on current topics within the applied industrial biocatalysis field and ... Leer más

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Provides clear and comprehensive coverage of recently developed applied biocatalysis for synthetic organic chemists with an emphasis to promote green chemistry in pharmaceutical and process chemistryThis book aims to make biocatalysis more accessible to both academic and industrial synthetic organic chemists. It focuses on current topics within the applied industrial biocatalysis field and
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Heterologous Expression of Membrane Proteins

Methods and Protocols

Edición de Isabelle Mus-Veteau ...

Series series Springer Protocols
This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus ... Leer más

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This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus
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Enzyme-Mediated Ligation Methods

Edición de Timo Nuijens, Marcel Schmidt ...

Series series Springer Protocols
This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and ... Leer más

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This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and
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Mitochondrial Medicine

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Series series Springer Protocols
This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume II guides readers through chapters on mitochondrial dysfunction, functional’ mitochondria, mitochondrial retrograde, mitochondrial dNTP pool quantification, mitochondrial ADP-ribosylation, blue-native gel approach, 3D ... Leer más

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This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume II guides readers through chapters on mitochondrial dysfunction, functional’ mitochondria, mitochondrial retrograde, mitochondrial dNTP pool quantification, mitochondrial ADP-ribosylation, blue-native gel approach, 3D
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Metabolic Reprogramming

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Series series Springer Protocols
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This volume provides state-of-the-art methods and protocols from researchers in the field of metabolism. Chapters guide readers through methodologies measuring different aspects of lipid metabolism, quantifying the amino acids, mitochondrial and intracellular by-products, detect enzyme and kinase activities, detecting a variety of metabolic pathways, and intracellular networks including nucleotide
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Series series Springer Protocols
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This volume covers the latest key aspects of therapeutic protein applications. Chapters in this book cover topics such as the discovery, production, and conjugation of protein-proteins with discussions on the direction of future development and advancements; ways to use these engineering proteins for therapeutic and vaccine applications; and the use of modified protein nanocarriers. Written in the
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Proteases and Cancer

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Edición de Salvatore Santamaria ...

Series series Springer Protocols
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This volume details bioinformatics analysis, biochemical assays, recombinant protein expression and purification, methods to investigate protease activity in cell-based, organoids and in vivo systems, proteomics, transcriptomics, machine learning and novel approaches to target dysregulated protease activity in cancer. Written in the highly successful Methods in Molecular Biology series format,
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Lysosomes

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Edición de Stephanie M. Hughes, Indranil Basak ...

Series series Springer Protocols
This volume provides a wide range of methods to discover and assess lysosome structure and function are provided. Chapters detail the essential methodologies and giving expert insight to key features with notes pertaining to key elements of the methods.Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions tothe material, lists of ... Leer más

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This volume provides a wide range of methods to discover and assess lysosome structure and function are provided. Chapters detail the essential methodologies and giving expert insight to key features with notes pertaining to key elements of the methods.Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions tothe material, lists of
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Recombinant Protein Expression in Mammalian Cells

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This fully updated volume explores notable developments in the field of mammalian cell-based recombinant protein production. Beginning with methods for transient recombinant protein production, the book continues with methods for stable cell pool generation, protein production using stable clonal cell lines, as well as high-throughput screening technologies for characterizing transient cell ... Leer más

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This fully updated volume explores notable developments in the field of mammalian cell-based recombinant protein production. Beginning with methods for transient recombinant protein production, the book continues with methods for stable cell pool generation, protein production using stable clonal cell lines, as well as high-throughput screening technologies for characterizing transient cell
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Mass Spectrometry-Based Proteomics

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Heterologous Expression of Membrane Proteins

Enzyme-Mediated Ligation Methods

Mitochondrial Medicine

Metabolic Reprogramming

Therapeutic Proteins

Proteases and Cancer

Lysosomes

Recombinant Protein Expression in Mammalian Cells