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Springer Protocols e-Bøker Serie

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  • Zebrafish

    Methods and Protocols

    Serie serien Springer Protocols
    This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the ... Les mer

    2 107,88 kr

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  • Culture of Animal Cells

    A Manual of Basic Technique and Specialized Applications

    Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made – particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in ... Les mer

    967,99 kr

  • The AGT Cytogenetics Laboratory Manual

    Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics.Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis ... Les mer

    2 046,69 kr

  • Drosophila

    Methods and Protocols

    Redigert av Christian Dahmann ...
    Serie serien Springer Protocols
    This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and ... Les mer

    1 311,05 kr

  • Antibody Engineering Volume 1

    Serie serien Springer Protocols
    Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new ... Les mer

    3 658,28 kr

  • In Vitro Bioassay Techniques for Anticancer Drug Discovery and Development

    This comprehensive and useful handbook represents a definitive up-to-date compendium of key in vitro bioassay methods that are employed to quantify and validate the anticancer activity of a drug candidate before it makes its way in to animal or clinical trials.In Vitro Bioassay Techniques for Anticancer Drug Discovery and Development covers the screening and evaluation of potential drug candidates ... Les mer

    534,52 kr

  • Cancer Cell Biology

    Methods and Protocols

    Redigert av Sherri L. Christian ...
    Serie serien Springer Protocols
    This volume provides detailed methods on the mechanisms of underlying cancer cell biology. Chapters guide readers through techniques for culturing cancer cell lines, xenografts, cryopreservation of tumor cells, analyzing the co-culture of breast cancer cells, protein secretion by ELISA, flow cytometry-based, multi-parametric immunofluorescence analysis, protein expression by western blot, analysis ... Les mer

    1 618,60 kr

  • Skeletal Muscle Stem Cells

    Methods and Protocols

    Redigert av Atsushi Asakura ...
    Serie serien Springer Protocols
    This volume looks at the latest technologies and methods--combined with new genetic tools available in animal models--used in this constantly evolving field. The chapters in this book are organized into three sections: Section one covers muscle stem cells and progenitor cells; Section Two discusses animal models for muscle stem cells and regeneration; and Section Three explores bioinformatics and ... Les mer

    1 411,84 kr

  • Emerging Model Organisms

    Redigert av Wei Wang, Nicolas Rohner, Yongfu Wang ...
    Serie serien Springer Protocols
    This volume discusses different approaches towards understanding the brain plasticity, aging, and regeneration of the nervous system in model organisms. Chapters guide the reader through an overview of model organisms, setup of an aquatic system, Tol2-mediated transgenesis, CRISPR/Cas9-mediated gene knock-out and knock-in, Cas13d-mediated gene knockdown, tissue clearing and expansion methods for ... Les mer

    1 618,60 kr

  • The ESCRT Complexes

    Methods and Protocols

    Redigert av Emmanuel Culetto, Renaud Legouis ...
    Serie serien Springer Protocols
    This detailed collection gathers both established and recent technical procedures to study the Endosomal Sorting Complex Required for Transport (ESCRT) complexes in a wide range of biological systems: Archaea, A. thaliana, U. maydis, S. cerevisiae, S. pombe, C. elegans, D. melanogaster, and mammalian cells. Opening with a section on imaging techniques, the book continues with chapters covering ... Les mer

    1 079,03 kr

  • The P2X7 Receptor

    Methods and Protocols

    Redigert av Annette Nicke ...
    Serie serien Springer Protocols
    This detailed volume covers diverse aspects of P2X7 receptor analysis, ranging from its molecular structure to related pharmacological and immunological tools, via its analysis in heterologous expression systems as well as assays using primary cells and whole animal models. After three introductory chapters that focus on its structure, ligands, and physiological functions, the book details the ... Les mer

    1 618,60 kr

  • Alzheimer’s Disease

    Methods and Protocols

    Redigert av Jerold Chun ...
    Serie serien Springer Protocols
    This volume explores the latest techniques used to study the human brain towards understanding Alzheimer’s Disease and related neurodegenerative disorders. Contributed to by world-renowned experts, the chapters in this book are divided into five parts. Part One discusses human post-mortem brain preparations including single-cell isolation and use of specialized imaging. Part Two talks about neural ... Les mer

    1 618,60 kr