Since most therapeutic efforts have been predominantly focused on pharmaceuticals that target proteins, there is an unmet need to develop drugs that intercept cellular pathways that critically involve nucleic acids. Progress in the discovery of nucleic acid binding drugs naturally relies on the availability of analytical methods that assess the eff

This book spans diverse aspects of modified nucleic acids, from chemical synthesis and spectroscopy to in vivo applications, and highlights studies on chemical modifications of the backbone and nucleobases. Topics discussed include fluorescent pyrimidine and purine analogs, enzymatic approaches to the preparation of modified nucleic acids, emission and electron paramagnetic resonance (EPR) spectroscopy for studying nucleic acid structure and dynamics, non-covalent binding of low- and high-...

THE LATEST BREAKTHROUGHS IN COMPUTER-AIDED DRUG DESIGN AND DELIVERYThis definitive text provides in-depth information on computer-assisted techniques for discovering, designing, and optimizing new, effective, and safe drugs. Computer-Aided Drug Design and Delivery Systems offers objective and quantitative data on the use and delivery of drugs in humans. Enabling technologies such as bioinformatics, pharmacokinetics, biosensors, robotics, and bioinstruments are thoroughly...

Patents protecting biotechnological invention are becoming ever more important. Because biotechnology has many differences with respect to other technologies, lessons learned in other fields of technology cannot simply be transferred to adopt a suitable strategy for dealing with biotechnology inventions.In this volume, general aspects of biopatent law will be discussed. This involves questions of patentability, including ethical issues and issues of technicality, as well as questio...

State-of-the-art nanostructuring principles, methods, and aplicationsSynthesize, characterize, and deploy highly miniaturized components using the theories and techniques contained in this comprehensive resource. Written by a nanotechnology expert, this authoritative volume covers the latest advances along with detailed schematics and real-world applications in engineering and the life sciences. Inside, 37 different nanostructuring methods and 16 different kinds of...

The book focuses on the aqueous interface of biomolecules, a vital yet overlooked area of biophysical research. Most biological phenomena cannot be fully understood at the molecular level without considering interfacial behavior.The author presents conceptual advances in molecular biophysics that herald the advent of a new discipline, epistructural biology, centered on the interactions of water and bio molecular structures across the interface. The author introduces powerf...

This book introduces key fundamentals of microarray bioprinting, including the required chip platforms and associated instruments/devices, experimental protocols for cell printing and biochemical- and cell-based assays, and several example applications. Various bioprinting approaches that allow for the rapid testing of hundreds of different cell culture conditions in combinations on a single chip are discussed in detail. Also covered is high-content, 3D cell-based imaging assays of tissue ...

This book delves into the recent developments in the microscale and microfluidic technologies that allow manipulation at the single and cell aggregate level. Expert authors review the dominant mechanisms that manipulate and sort biological structures, making this a state-of-the-art overview of conventional cell sorting techniques, the principles of microfluidics, and of microfluidic devices. All chapters highlight the benefits and drawbacks of each technique they discuss, which include mag...

In the context of the continual increase in the global incidence of diabetes, this book focuses on particular aspects of the disease such as the socio-economic burden and the effects on individuals and their families. It addresses a wide range of topics regarding its physiological relevance, metabolic angles, biochemistry, and discusses current and upcoming treatment approaches. It is unique in offering a chapter dedicated to herbal remedies for diabetes. Appealing to a broad readership, i...

This book presents the fundamentals and applications of Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-ToF-MS) technique. It highlights the basic principles, the history of invention as well as the mechanism of ionization and mass determination using this technique. It describes the fundamental principles and methods for MALDI spectra interpretation and determination of exact chemical structures from experimental data. This book guides the reader throug...

Flow Control Methods and Devices in Micrometer Scale Channels, by Shuichi Shoji and Kentaro Kawai. Micromixing Within Microfluidic Devices, by Lorenzo Capretto, Wei Cheng, Martyn Hill and Xunli Zhang. Basic Technologies for Droplet Microfluidics, by Shaojiang Zeng, Xin Liu, Hua Xie and Bingcheng Lin. Electrorheological Fluid and Its Applications in Microfluidics, by Limu Wang, Xiuqing Gong and Weijia Wen. Biosensors in Microfluidic Chips, by Jongmin Noh, Hee Chan Kim and Taek Dong Chung. A...

This lavishly-illustrated, authoritative atlas explores the intricate art of culturing human pluripotent stem cells. Twelve chapters – containing more than 280 color illustrations – cover a variety of topics in pluripotent stem cell culturing including mouse and human fibroblasts, human embryonic stem cells and induced pluripotent stem cells, characteristic staining patterns, and abnormal cultures, among others. Atlas of Human Pluripotent Stem Cells in Culture is a comprehensive c...