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Duchenne Muscular Dystrophy

Methods and Protocols

Edited by Kevin Flanigan, Stefan Nicolau ...

Series series Springer Protocols
This volume discusses protocols for key laboratory techniques used to study Duchenne and Becker muscular dystrophies. The chapters in this book cover a wide array of topics such as biochemical assays, quantification of dystrophin and related proteins, and measurements of muscle physiology. The chapters also cover methods of DMD sequence analysis; processing and isolation of cells from muscle ... Read more

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This volume discusses protocols for key laboratory techniques used to study Duchenne and Becker muscular dystrophies. The chapters in this book cover a wide array of topics such as biochemical assays, quantification of dystrophin and related proteins, and measurements of muscle physiology. The chapters also cover methods of DMD sequence analysis; processing and isolation of cells from muscle
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Neuromuscular Assessments of Form and Function

Edited by Philip J. Atherton, Daniel J. Wilkinson ...

Series series Springer Protocols
This volume looks at the latest methods used to study imaging techniques, metabolic tracing, and deep muscle phenotyping. The chapters in this book cover topics such as imaging skeletal muscle mass using MRI, dual-energy x-ray absorptiometry, CT, and ultrasound; measures of neuromuscular function such as power/strength/force; microscopy and immunohistochemistry; and ex vivo fibre function and ... Read more

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This volume looks at the latest methods used to study imaging techniques, metabolic tracing, and deep muscle phenotyping. The chapters in this book cover topics such as imaging skeletal muscle mass using MRI, dual-energy x-ray absorptiometry, CT, and ultrasound; measures of neuromuscular function such as power/strength/force; microscopy and immunohistochemistry; and ex vivo fibre function and
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Current Laboratory Techniques in Rabies Diagnosis, Research and Prevention, Volume 1

Edited by Thirumeni Nagarajan, Charles Rupprecht ...

Laboratory Techniques in Rabies Diagnosis, Research and Prevention provides a basic understanding of the current trends in rabies. It establishes a new facility for rabies surveillance, vaccine and antibody manufacturing. It offers clarity about the choice of laboratory methods for diagnosis and virus typing, of systems for producing monoclonal and polyclonal antibodies and of methods for testing ... Read more

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Laboratory Techniques in Rabies Diagnosis, Research and Prevention provides a basic understanding of the current trends in rabies. It establishes a new facility for rabies surveillance, vaccine and antibody manufacturing. It offers clarity about the choice of laboratory methods for diagnosis and virus typing, of systems for producing monoclonal and polyclonal antibodies and of methods for testing
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Tertiary Lymphoid Structures

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Edited by Marie-Caroline Dieu-Nosjean ...

Series series Springer Protocols
This second edition details new and updated chapters on technologies related to tertiary lymphoid structures( TLS). Chapters guide readers through TLS in cancers, different stages of TLS development, immune function of TLS, molecular, cellular, and imaging methods, and preclinical methods to uncover the function of cells composing TLS in inflammatory situations in humans. Written in the highly ... Read more

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This second edition details new and updated chapters on technologies related to tertiary lymphoid structures( TLS). Chapters guide readers through TLS in cancers, different stages of TLS development, immune function of TLS, molecular, cellular, and imaging methods, and preclinical methods to uncover the function of cells composing TLS in inflammatory situations in humans. Written in the highly
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Antibody Techniques

Edited by Vedpal S. Malik, Erik P. Lillehoj ...

The applicability of immunotechniques to a wide variety of research problems in many areas of biology and chemistry has expanded dramatically over the last two decades ever since the introduction of monoclonal antibodies and sophisticated immunosorbent techniques. Exquisitely specific antibody molecules provide means of separation, quantitative and qualitative analysis, and localization useful to ... Read more

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The applicability of immunotechniques to a wide variety of research problems in many areas of biology and chemistry has expanded dramatically over the last two decades ever since the introduction of monoclonal antibodies and sophisticated immunosorbent techniques. Exquisitely specific antibody molecules provide means of separation, quantitative and qualitative analysis, and localization useful to
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Muscular Dystrophy Therapeutics

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Edited by Rika Maruyama, Toshifumi Yokota ...

Series series Springer Protocols
This detailed book presents a comprehensive collection of state-of-the-art protocols on muscular dystrophy therapeutics, covering therapeutics using antisense oligonucleotides, gene replacement, genome editing, small molecules, stem cells, and antibodies. Written by leaders in the field, the volume explores techniques that are currently in use and are starting an exciting therapeutic revolution in ... Read more

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This detailed book presents a comprehensive collection of state-of-the-art protocols on muscular dystrophy therapeutics, covering therapeutics using antisense oligonucleotides, gene replacement, genome editing, small molecules, stem cells, and antibodies. Written by leaders in the field, the volume explores techniques that are currently in use and are starting an exciting therapeutic revolution in
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Bacterial Pathogenesis

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Series series Springer Protocols
This detailed volume presents a diverse set of methodological approaches designed to improve our understanding of bacterial infections from a wide range of bacterial species. Beginning with biofilms and subcellular compartments, the book explores transcriptional analysis, methods for studying plasmid dynamics, and tools for phylogenetic analysis of bacterial genomes, as well as bacterial effector ... Read more

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This detailed volume presents a diverse set of methodological approaches designed to improve our understanding of bacterial infections from a wide range of bacterial species. Beginning with biofilms and subcellular compartments, the book explores transcriptional analysis, methods for studying plasmid dynamics, and tools for phylogenetic analysis of bacterial genomes, as well as bacterial effector
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Malaria Immunology

Targeting the Surface of Infected Erythrocytes

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This volume covers a broad range of methods, technologies, and protocols on malaria. Chapters detail research on collecting parasites in the field, single molecule-level analyses of adhesive interactions, and focused studies aiming at disrupting the devastating disease. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the ... Read more

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This volume covers a broad range of methods, technologies, and protocols on malaria. Chapters detail research on collecting parasites in the field, single molecule-level analyses of adhesive interactions, and focused studies aiming at disrupting the devastating disease. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the
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This detailed volume aims to elucidate the molecular mechanisms that underlie antifungal resistance. The book highlights methods to identify and characterize antifungal activity, to define and characterize strains with altered responses to antifungal drugs, to investigate the genetic and molecular mechanisms of these alterations of antifungal drug susceptibility, and, finally, to approach the
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Tissue-Resident Macrophages

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This detailed book delves into the diverse techniques and applications to target, isolate, image, phenotype, and analyze tissue-resident and monocyte-derived macrophages. The contents aim to describe the current knowledge about macrophage development and function which forces the scientific field to move beyond the previously described M1/M2 macrophage paradigm to be able to dissect macrophage ... Read more

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This detailed book delves into the diverse techniques and applications to target, isolate, image, phenotype, and analyze tissue-resident and monocyte-derived macrophages. The contents aim to describe the current knowledge about macrophage development and function which forces the scientific field to move beyond the previously described M1/M2 macrophage paradigm to be able to dissect macrophage
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Candida Species

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Edited by Richard Calderone ...

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This updated volume explores Candida and candidiasis methods, useful to a wide variety of Candida scientists including those new to the field. Beginning with a section on the Candida genome, the book continues by covering techniques for antifungal discovery and studying resistance, pathogenesis and virulence, communities, as well as immunity. Biofilm protocols are also featured. Written for the
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This detailed volume provides methods to guide assay development, procedures designed to investigate the chemokine and glycosaminoglycan (GAG) networks, as well as their interactions, in a wide range of organs and tissues in disease and in health. The initial chapters in this book present in vivo models used to examine the roles of chemokines and GAGs in normal physiology and in the
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