Showing results for "Cellular Programming and Reprogramming Methods and Protocols Sheng Ding German"

This third edition provides new and updated experimental and computational methods designed to study transcriptional regulation in stem cells and related contexts. Chapters highlight genomic profiling, single cell and multiomic technologies, chromatin and spatial analysis, and computational modeling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readi...

**This third edition expands upon the chapters of the previous editions, providing new and updated methods. Chapters explore protein-lipid interactions, membranes, membrane protein structure, how membrane proteins affect lipids and how they are in turn affected by the lipid bilayer, and lipid properties. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagen...

This detailed volume presents experimental and computational methods for constructing and interrogating synthetic gene circuits across multiple organizational scales. Beginning with a section on genetic tools, platforms, and experimental frameworks that enable sophisticated circuit construction, the book continues by covering methods for regulating circuit behavior and mitigating variability, as well as frameworks for understanding time-dependent circuit behavior in growing cells and popul...

This volume details computational tools for the study of protein evolution based on 1D-sequence and 3D-structure databases. Chapters guide readers through the analysis of 1D protein sequences for coevolutionary fold prediction, protein-nucleic acid interactions, distant homologs and functional motifs, characterization of fold energetics, disordered proteins, mutagenesis-driven modulation of conformational landscapes, 3D protein domains, prediction of 3D protein-protein interactions, and hy...

This book presents a major research program on epigenetic mechanisms of cell programming and reprogramming. The program was launched by the National Natural Science Foundation of China during the 11th Five-Year Plan period, which was started in October 2008 and concluded at the end of 2016. It first summarizes the epigenetic research plans and the current situation of epigenetic research in China and the international frontier. With the application of interdisciplinary research methods, th...

This detailed volume explores techniques for the study of neurogenic niches and neural lineage progression, specifically addressing major methodological challenges in studying neural stem cells (NSCs) and related cell populations, such as astrocytes, oligodendrocytes, and neurons. The chapters are organized to offer a comprehensive technical foundation, focusing on isolation and identification, monitoring, manipulation, analysis, and the exploration of potential therapeutic applications of...

This second edition details a collection of state-of-art methods including identification of novel ncRNAs and their targets, functional annotation and disease association in different biological contexts. Chapters guide readers through an overview of disease-specific ncRNAs, computational methods and workflows for ncRNA discovery, annotation based on high-throughput sequencing data, bioinformatics tools and databases for ncRNA analyses, network-based methods, and kinetic modelling of ncRNA...

This volume details molecular mechanisms that are involved in neuronal morphogenesis, and methods for analyzing these mechanisms. Chapters detail analyzing neuronal morphology in vitro and in vivo using a variety of model systems, including rodents and human induced pluripotent stem cells (hiPSCs). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, st...

This detailed volume presents a comprehensive guide to live-cell RNA imaging techniques and methodologies that have been successfully used to study the dynamics or function of various RNAs in cells. The topics covered encompass techniques including the detection of aptamer-tagged mRNA molecules utilizing fluorogenic proteins, monitoring endogenous mRNA via a split-protein-based probe within neuronal cells, sensitive detection of low-abundance RNAs employing catalytic hairpin assembly circu...

This volume covers the latest advancements in the study of lipid signaling. The chapters in this book cover topics such as neuronal cells such as retinal ganglion cells, microglial cells to muscle, and epithelial cells; extraction for signaling studies, including clinical studies in humans; and discussions on different cells, tissues, and diseases like Alzheimer’s disease, musculoskeletal diseases, eye diseases, and metabolic diseases. Written in the highly successful Methods in Molecu...

This volume covers the latest key aspects of therapeutic protein applications. Chapters in this book cover topics such as the discovery, production, and conjugation of protein-proteins with discussions on the direction of future development and advancements; ways to use these engineering proteins for therapeutic and vaccine applications; and the use of modified protein nanocarriers. Written in the highly successful Methods in Molecular Biology series format, chapters include intro...

This fully updated volume explores notable developments in the field of mammalian cell-based recombinant protein production. Beginning with methods for transient recombinant protein production, the book continues with methods for stable cell pool generation, protein production using stable clonal cell lines, as well as high-throughput screening technologies for characterizing transient cell surface protein ectodomain expression and for identifying host genes involved in protein production....