Optical Imaging Techniques in Cell Biology, Second Edition covers the field of biological microscopy, from the optics of the microscope to the latest advances in imaging below the traditional resolution limit. It includes the techniques-such as labeling by immunofluorescence and fluorescent proteins-which have revolutionized cell biology. Quantitat

Fluorescence imaging, at macro, micro, and submicro scales, has revolutionized biological science in the past 30 years. Immunolabelling has provided precise targeting of molecules in fixed tissue, while fluorescent proteins have enabled localization in living tissues. Fluorescent indicators enable imaging of dynamic changes in cell metabolism.This book covers, for the first time, imaging at all scales from macro to submicro (superresolution). Its authors include Robert Clegg, legen...

This book looks at the comprehensive management of all aspects of gallstone disease, from the epidemiology and pathogenesis to the complex management approaches required for some patients.The main emphasis is placed on effective diagnosis and treatment, making extensive use of practical case-based material while reviewing the guidelines in a multi-disciplinary manner.It is an essential read for all clinicians involved in managing patients with gallstone disease.