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  • Mitotic Exit

    Methods and Protocols

    Bewerkt door Victor M. Bolanos-Garcia ...
    Serie series Springer Protocols
    This volume explores the latest approaches and techniques used to study mitotic exit in diverse model organisms. The chapters in this book cover topics such as transgenic methods generation of RNAi-sensitive cell lines; gene overexpression in heterologue gene expression systems; quantitative live cell imaging and FRET-FLIM; biochemical protocols for analyzing post-translational modifications ... Meer lezen

    232,14 €

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  • PCR Guru

    An Ultimate Benchtop Reference for Molecular Biologists

    PCR Guru: An Ultimate Benchtop Reference for Molecular Biologists is provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with PCR setup and optimization. As a laboratory guide, it emphasizes the technical aspects of employing PCR as a tool in molecular biology laboratories. The book covers the history of PCR and the basic ... Meer lezen

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  • Zebrafish

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    This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the ... Meer lezen

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  • Histones

    Methods and Protocols

    Bewerkt door Luc Gaudreau, Benoit Guillemette ...
    Serie series Springer Protocols
    This second edition provides new and updated chapters on histones for molecular biologists, biochemists and geneticists. Chapters cover protein complexes that modify chromatin either by adding or removing post-translational modifications, or by exchanging histone variants within the nucleosome. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief ... Meer lezen

    242,74 €

  • DNA-Protein Interactions

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    Bewerkt door Marcos Simoes-Costa ...
    Serie series Springer Protocols
    This volume details protocols emphasizing systems-level approaches that can be applied to genomic analyses. Chapters detail techniques for optimized application in in vivo systems, spatial, physiological, environmental contexts, imaging-based techniques, single-molecule approaches, CRISPR systems, new genomic approaches, and measurements of kinetics governing. Written in the format of the highly ... Meer lezen

    128,78 €

  • Heterologous Expression of Membrane Proteins

    Methods and Protocols

    Bewerkt door Isabelle Mus-Veteau ...
    Serie series Springer Protocols
    This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus ... Meer lezen

    158,99 €

  • Single-Domain Antibodies

    Methods and Protocols

    Bewerkt door Greg Hussack, Kevin A. Henry ...
    Serie series Springer Protocols
    This volume covers current and emerging techniques for studying single-domain antibodies (sdAbs). Chapters guide readers through the biology and immunology of sdAbs in camelids and sharks, isolation of sdAbs, protein engineering approaches to optimize the solubility, stability, valency and antigen binding affinity of sdAbs, and specialized applications of sdAbs. Written in the format of the highly ... Meer lezen

    190,79 €

  • Recombinant Protein Expression in Mammalian Cells

    Methods and Protocols

    Bewerkt door David L. Hacker ...
    Serie series Springer Protocols
    This fully updated volume explores notable developments in the field of mammalian cell-based recombinant protein production. Beginning with methods for transient recombinant protein production, the book continues with methods for stable cell pool generation, protein production using stable clonal cell lines, as well as high-throughput screening technologies for characterizing transient cell ... Meer lezen

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  • Therapeutic Proteins

    Methods and Protocols

    Serie series Springer Protocols
    This volume covers the latest key aspects of therapeutic protein applications. Chapters in this book cover topics such as the discovery, production, and conjugation of protein-proteins with discussions on the direction of future development and advancements; ways to use these engineering proteins for therapeutic and vaccine applications; and the use of modified protein nanocarriers. Written in the ... Meer lezen

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  • DNA Damage Detection

    Methods and Protocols

    Bewerkt door Takamitsu A. Kato ...
    Serie series Springer Protocols
    This volume explores techniques in DNA damage analysis, important not only for understanding cellular health after exposure to genotoxic agents but also for assessing cellular ability of genome maintenance. The book explores traditional DNA damage measurement methods, as well as modern fluorescence-based analysis and high throughput automatic analysis methods. Written for the highly successful ... Meer lezen

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  • Base Excision Repair Pathway

    Methods and Protocols

    Bewerkt door Kishor K. Bhakat, Tapas K. Hazra ...
    Serie series Springer Protocols
    This detailed volume provides a comprehensive set of experimental protocols and useful strategies to examine the repair of damaged bases via the Base Excision Repair (BER) pathway in vitro and in cells. Beginning with multiple molecular and cellular techniques to examine the excision of damaged bases from double-stranded DNA or DNA wrapped in a nucleosome, the book continues with sections covering ... Meer lezen

    148,59 €

  • Mitochondrial Medicine

    Volume 2: Assessing Mitochondria

    Bewerkt door Volkmar Weissig, Marvin Edeas ...
    Serie series Springer Protocols
    This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume II guides readers through chapters on mitochondrial dysfunction, functional’ mitochondria, mitochondrial retrograde, mitochondrial dNTP pool quantification, mitochondrial ADP-ribosylation, blue-native gel approach, 3D ... Meer lezen

    118,87 €