This book contains the proceedings of the Symposium on Systems Theory in Data and Optimization (SysDO) held in Stuttgart, Germany, from 30th September to 2nd October 2024. It addresses theoretical and practical research questions arising at the intersection of systems and control theory, data, and optimization. The increasing prevalence of cyber-physical systems sparks the need for new methods to integrate measured data and different forms of feedback, especially optimization-based feedbac...