This volume provides state-of-the-art methods and protocols from researchers in the field of metabolism. Chapters guide readers through methodologies measuring different aspects of lipid metabolism, quantifying the amino acids, mitochondrial and intracellular by-products, detect enzyme and kinase activities, detecting a variety of metabolic pathways, and intracellular networks including nucleotide metabolism and the metabolic response to hypoxia and cytotoxic drugs. Written in the format o...

Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics.Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis of G-banded chromosomes, specialized stains that address specific chromosomal structur...

The rapid growth of interest and research activity in ion channels is indicative of their fundamental importance in the maintenance of the living state. This volume was prepared with a view toward providing a sampling of the range of molecular and physical methods that are significant for the study of ion channels. As part of the Reliable Lab Solutions series, Essential Ion Channel Methods brings together chapters from volumes 293 and 294 of Methods in Enzymology. The chapters have been se...

Photoreceptor Cells, Volume 15 covers the advances in the study of photoreceptor cells. The book discusses biochemical, cell and structural biological, and molecular biological techniques needed for the study of photoreceptor cells. The text also describes the methods for observing the structure of photoreceptor cells and for studying their metabolism. The procedures for photoreceptor cell isolation are also considered. The book further tackles the preparation of proteins involved in rod c...

Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest "hands on" knowledge of key laboratories in this st...

This comprehensive and useful handbook represents a definitive up-to-date compendium of key in vitro bioassay methods that are employed to quantify and validate the anticancer activity of a drug candidate before it makes its way in to animal or clinical trials.In Vitro Bioassay Techniques for Anticancer Drug Discovery and Development covers the screening and evaluation of potential drug candidates in a wide category of anticancer assays demonstrating the s...

Protein Liquid Chromatography is a handbook-style guide to liquid chromatography as a tool for isolating and purifying proteins, consisting of 25 individual chapters divided into three parts: Part A covers commonly-used, classic modes of chromatography such as ion-exchange, size-exclusion, and reversed-phase; Part B deals with various target protein classes such as membrane proteins, recombinant proteins, and glycoproteins; and Part C looks at various miscellaneous related topics, includin...

Cytometry is characterization and measurement of cells and cellular constituents, most often used to immunophenotype cells - that is, to distinguish healthy cells from diseased cells. Flow Cytometry specifically is quite sensitive, allowing researchers to detect rare cell types and residual levels of disease, and as such has been the method of choice for important studies such as monitoring the blood of AIDS patients. For this reason, there is a great need for a practical, comprehensive ma...

This volume provides the most widely used protocols and techniques employed in lipopolysaccharide( LPS) transport studies. Chapters detail identification and analysis of essential genes and crucial mutations in LPS transport-related genes, LPS profile determination, LPS chemical analysis, protein-LPS interaction, and LPS visualization. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists neces...

This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume II guides readers through chapters on mitochondrial dysfunction, functional’ mitochondria, mitochondrial retrograde, mitochondrial dNTP pool quantification, mitochondrial ADP-ribosylation, blue-native gel approach, 3D optical cryo-imaging method, mitochondrial ATP and ROS production, protocol for untargeted metabo...

This volume details comprehensive protocols and methodologies to assess mitochondrial bioenergetics and dynamics in different tissues and cells involving health and pathological states. Chapters guide readers through methods for assessment of the energy metabolism including Oxygen Consumption Rate (OCR), mitochondrial membrane potential, and measuring mitochondrial Ca2+ handling, and ROS emission. Written in the format of the highly successful Methods in Molecular Biology series, ...

This fully updated volume assembles a comprehensive collection of methods, techniques, and strategies to investigate the molecular and cellular biology of peroxisomes in different organisms. Peroxisome research is on the rise, as novel functions and proteins of this dynamic organelle are still being discovered through studies in model systems including humans, mice, flies, plants, fungi, and yeast, and this progress is reflected in the chapters included in this collection. Written for the ...