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  • MicroRNA Detection and Target Identification

    Methods and Protocols

    Redigeret af Tamas Dalmay ...
    Serier serie Springer Protocols
    This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe ... Læs mere

    1.021,24 kr.

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  • Drosophila

    Methods and Protocols

    Redigeret af Christian Dahmann ...
    Serier serie Springer Protocols
    This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and ... Læs mere

    1.021,24 kr.

  • Catharanthus roseus

    Methods and Protocols

    Serier serie Springer Protocols
    This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in C. roseus from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, transcription factor characterization up to functional genomic tools based on gene overexpression. Written in the format of the highly successful Methods in ... Læs mere

    1.867,83 kr.

  • A Practical Guide to Protein Engineering

    Serier serie Biomedical and Life Sciences (R0)
    This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a ... Læs mere

    747,08 kr.

  • Directed Evolution

    Methods and Protocols

    Redigeret af Andrew Currin, Neil Swainston ...
    Serier serie Springer Protocols
    This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using ... Læs mere

    1.428,99 kr.

  • Alternative Splicing

    Methods and Protocols

    Redigeret af Peter Scheiffele, Oriane Mauger ...
    Serier serie Springer Protocols
    This detailed volume collects commonly used and cutting-edge methods to analyze alternative splicing, a key step in gene regulation. After an introduction of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in complex biological systems, visualizing and localizing alternative spliced ... Læs mere

    1.400,85 kr.

  • piRNA

    Methods and Protocols

    Serier serie Springer Protocols
    This detailed volume explores newly-developed methods in PIWI-interacting RNAs (piRNAs) research, methods currently applied to other ncRNAs involved in nuclear regulation which can be used to study piRNAs, and piRNA methods applied in non-classical organisms. It also includes several bioinformatic and biophysical methods related to piRNA studies, consistent with the increasing importance of high ... Læs mere

    1.260,86 kr.

  • Spatial Genome Organization

    Methods and Protocols

    Redigeret af Tom Sexton ...
    Serier serie Springer Protocols
    This detailed volume explores a variety of cutting-edge techniques used to interrogate spatial genome organization. Beginning with a section covering the vital chromosome conformation capture (3C) technique, this collection continues with chapters on targeted Hi-C approaches, sequencing-based approaches to assess nuclear environment, as well as single-cell technologies to better characterize the ... Læs mere

    1.400,85 kr.

  • The Proteomics Protocols Handbook

    Redigeret af John M. Walker ...
    Serier serie Springer Protocols
    Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying ... Læs mere

    2.429,24 kr.

  • DNA-Encoded Chemical Libraries

    Methods and Protocols

    Redigeret af David Israel, Yun Ding ...
    Serier serie Springer Protocols
    This volume discusses protocols that cover synthesis, screening by selection, and analysis of DNA-encoded chemical libraries (DEL). Chapters in this book focus on methods used to practice DEL technology and include solution phase library synthesis using a variety of chemistries; DNA encoding of chemical structure; design, preparation and analysis of target proteins and tool compounds; screening of ... Læs mere

    840,49 kr.

  • Protein-Protein Interactions

    Methods and Protocols

    Redigeret af Shahid Mukhtar ...
    Serier serie Springer Protocols
    This detailed volume provides a comprehensive collection of classic and cutting-edge methods and techniques in mapping protein-protein interactions. The chapters include a variety of in vitro and in vivo experimental methods covering cell biology, biochemistry, and biophysics. In addition, the book also explores in silico methods including sequence-, structure-, and phylogenetic profile-based ... Læs mere

    1.512,99 kr.

  • Plant Proteostasis

    Methods and Protocols

    Redigeret af L. Maria Lois, Marco Trujillo ...
    Serier serie Springer Protocols
    This second edition compiles state-of-art protocols to study the molecular mechanisms that determine proteome composition and contribute to maintaining protein homeostasis. Chapters describe methods that range from the in vitro analysis of enzyme cascades to real-time in vivo studies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their ... Læs mere

    1.260,86 kr.