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  • piRNA

    Methods and Protocols

    Serier serie Springer Protocols
    This detailed volume explores newly-developed methods in PIWI-interacting RNAs (piRNAs) research, methods currently applied to other ncRNAs involved in nuclear regulation which can be used to study piRNAs, and piRNA methods applied in non-classical organisms. It also includes several bioinformatic and biophysical methods related to piRNA studies, consistent with the increasing importance of high ... Læs mere

    1.260,86 kr.

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  • Bioinformatics and Functional Genomics

    The bestselling introduction to bioinformatics and genomics – now in its third editionWidely received in its previous editions, Bioinformatics and Functional Genomics offers the most broad-based introduction to this explosive new discipline. Now in a thoroughly updated and expanded third edition, it continues to be the go-to source for students and professionals involved in biomedical research ... Læs mere

    1.015,24 kr.

  • Drosophila

    Methods and Protocols

    Redigeret af Christian Dahmann ...
    Serier serie Springer Protocols
    This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and ... Læs mere

    1.021,24 kr.

  • Catharanthus roseus

    Methods and Protocols

    Serier serie Springer Protocols
    This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in C. roseus from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, transcription factor characterization up to functional genomic tools based on gene overexpression. Written in the format of the highly successful Methods in ... Læs mere

    1.867,83 kr.

  • A Practical Guide to Protein Engineering

    Serier serie Biomedical and Life Sciences (R0)
    This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a ... Læs mere

    747,08 kr.

  • Mitochondrial DNA

    Methods and Protocols

    Serier serie Springer Protocols
    This volume compiles a comprehensive range of methods to study key aspects of mitochondrial DNA including nucleoid structure and packaging, replication, genome integrity, and disease. Chapters are organized into eight methodological sections that cover in vitro and in vivo methods, including for mtDNA isolation, visualization, deep sequencing, gene editing, and diagnostic aspects of mtDNA disease. ... Læs mere

    1.756,86 kr.

  • R-Loops

    Methods and Protocols

    Redigeret af Andrés Aguilera, Alexey Ruzov ...
    Serier serie Springer Protocols
    This detailed book compiles a series of laboratory protocols covering the most important aspects of R-loop biology. Beginning with a range of methods allowing for the detection of DNA-RNA hybrids, as well as their purification and visualization by electron microscopy, the volume continues with methods based on the use of RNase H-derived tools to detect DNA-RNA hybrids in vitro and in vivo. Several ... Læs mere

    1.260,86 kr.

  • Spatial Genome Organization

    Methods and Protocols

    Redigeret af Tom Sexton ...
    Serier serie Springer Protocols
    This detailed volume explores a variety of cutting-edge techniques used to interrogate spatial genome organization. Beginning with a section covering the vital chromosome conformation capture (3C) technique, this collection continues with chapters on targeted Hi-C approaches, sequencing-based approaches to assess nuclear environment, as well as single-cell technologies to better characterize the ... Læs mere

    1.400,85 kr.

  • Prokaryotic Gene Regulation

    Methods and Protocols

    Redigeret af Eveline Peeters, Indra Bervoets ...
    Serier serie Springer Protocols
    This volume presents a collection of versatile methodologies to investigate prokaryotic gene regulation, with focus on the different levels of information processing and usefulness for various model organisms, whether archaeal, bacterial, or both. The chapters in this book are divided into four sections. Section One covers methods that enable the study of the structure of the bacterial/archaeal ... Læs mere

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  • Non-Ribosomal Peptide Biosynthesis and Engineering

    Methods and Protocols

    Serier serie Springer Protocols
    This volume provides new technologies on NRPSs and related carrier protein dependent synthases, including polyketide synthases (PKS) and fatty acid synthases (FAS). Chapters detail enzymology, structural biology, proteopromics, chemical biology, natural product chemistry, and bioinformatics. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes ... Læs mere

    1.176,74 kr.

  • The Mitoribosome

    Methods and Protocols

    Serier serie Springer Protocols
    This volume provides classic and new methods to study the structure, assembly pathway, and protein synthesis ability of mitoribosomes across species. Following an introduction of fundamental concepts on the topic, method chapters present detailed protocols based on cryo-electron tomography, cryo-EM approaches, mitoribosome purification techniques, mitochondrial translation assays, and methods to ... Læs mere

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  • Genotype Phenotype Coupling

    Methods and Protocols

    Redigeret af Stefan Zielonka, Simon Krah ...
    Serier serie Springer Protocols
    This detailed new edition broadens the scope of the first edition by moving beyond classical display technologies. This book explores methodologies for the generation of natively paired antibody libraries, single cell technologies, alternative scaffolds, and in silico antibody sequence assessments are described. The application of these methods may allow for a generation of improved therapeutics ... Læs mere

    1.428,99 kr.