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Genotype Phenotype Coupling

Methods and Protocols

Redigeret af Stefan Zielonka, Simon Krah ...

Serier serie Springer Protocols
This detailed new edition broadens the scope of the first edition by moving beyond classical display technologies. This book explores methodologies for the generation of natively paired antibody libraries, single cell technologies, alternative scaffolds, and in silico antibody sequence assessments are described. The application of these methods may allow for a generation of improved therapeutics ... Læs mere

1.428,99 kr.

This detailed new edition broadens the scope of the first edition by moving beyond classical display technologies. This book explores methodologies for the generation of natively paired antibody libraries, single cell technologies, alternative scaffolds, and in silico antibody sequence assessments are described. The application of these methods may allow for a generation of improved therapeutics
... Læs mere

Bioinformatics and Functional Genomics

af Jonathan Pevsner ...

The bestselling introduction to bioinformatics and genomics – now in its third editionWidely received in its previous editions, Bioinformatics and Functional Genomics offers the most broad-based introduction to this explosive new discipline. Now in a thoroughly updated and expanded third edition, it continues to be the go-to source for students and professionals involved in biomedical research ... Læs mere

1.015,24 kr.

The bestselling introduction to bioinformatics and genomics – now in its third editionWidely received in its previous editions, Bioinformatics and Functional Genomics offers the most broad-based introduction to this explosive new discipline. Now in a thoroughly updated and expanded third edition, it continues to be the go-to source for students and professionals involved in biomedical research
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The AGT Cytogenetics Laboratory Manual

Redigeret af Marilyn S. Arsham, Margaret J. Barch, Helen J. Lawce ...

Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics.Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis ... Læs mere

1.471,24 kr.

Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics.Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis
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Drosophila

Methods and Protocols

Redigeret af Christian Dahmann ...

Serier serie Springer Protocols
This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and ... Læs mere

1.021,24 kr.

This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and
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Antibody Engineering Volume 1

Redigeret af Roland E. Kontermann, Stefan Dübel ...

Serier serie Springer Protocols
Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new ... Læs mere

3.166,13 kr.

Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new
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Catharanthus roseus

Methods and Protocols

Redigeret af Vincent Courdavault, Sebastien Besseau ...

Serier serie Springer Protocols
This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in C. roseus from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, transcription factor characterization up to functional genomic tools based on gene overexpression. Written in the format of the highly successful Methods in ... Læs mere

1.867,83 kr.

This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in C. roseus from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, transcription factor characterization up to functional genomic tools based on gene overexpression. Written in the format of the highly successful Methods in
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Yeast Surface Display

Redigeret af Michael W. Traxlmayr ...

Serier serie Springer Protocols
This detailed volume explores a wide variety of applications of yeast surface display, an extensively used protein engineering technology. Beginning with detailed protocols for the construction and efficient selection/screening of yeast surface display libraries, as well as for the analysis of individual yeast-displayed protein variants, the book continues with protocols describing the selection ... Læs mere

1.681,11 kr.

This detailed volume explores a wide variety of applications of yeast surface display, an extensively used protein engineering technology. Beginning with detailed protocols for the construction and efficient selection/screening of yeast surface display libraries, as well as for the analysis of individual yeast-displayed protein variants, the book continues with protocols describing the selection
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Phage Display

Methods and Protocols

Redigeret af Michael Hust, Theam Soon Lim ...

Serier serie Springer Protocols
This second edition details new and updated methods on different antibody libraries, along with novel approaches for antibody discovery. Chapters focuses on the construction of antibody libraries, antibody expression, complementary approaches for antibody selection, and other phage display related applications. Written in the highly successful Methods in Molecular Biology series format, chapters ... Læs mere

2.045,38 kr.

This second edition details new and updated methods on different antibody libraries, along with novel approaches for antibody discovery. Chapters focuses on the construction of antibody libraries, antibody expression, complementary approaches for antibody selection, and other phage display related applications. Written in the highly successful Methods in Molecular Biology series format, chapters
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DNA-Protein Interactions

Methods and Protocols

Redigeret af Marcos Simoes-Costa ...

Serier serie Springer Protocols
This volume details protocols emphasizing systems-level approaches that can be applied to genomic analyses. Chapters detail techniques for optimized application in in vivo systems, spatial, physiological, environmental contexts, imaging-based techniques, single-molecule approaches, CRISPR systems, new genomic approaches, and measurements of kinetics governing. Written in the format of the highly ... Læs mere

1.021,24 kr.

This volume details protocols emphasizing systems-level approaches that can be applied to genomic analyses. Chapters detail techniques for optimized application in in vivo systems, spatial, physiological, environmental contexts, imaging-based techniques, single-molecule approaches, CRISPR systems, new genomic approaches, and measurements of kinetics governing. Written in the format of the highly
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Mitochondrial DNA

Methods and Protocols

Redigeret af Thomas J. Nicholls, Jay P. Uhler, Maria Falkenberg ...

Serier serie Springer Protocols
This volume compiles a comprehensive range of methods to study key aspects of mitochondrial DNA including nucleoid structure and packaging, replication, genome integrity, and disease. Chapters are organized into eight methodological sections that cover in vitro and in vivo methods, including for mtDNA isolation, visualization, deep sequencing, gene editing, and diagnostic aspects of mtDNA disease. ... Læs mere

1.756,86 kr.

This volume compiles a comprehensive range of methods to study key aspects of mitochondrial DNA including nucleoid structure and packaging, replication, genome integrity, and disease. Chapters are organized into eight methodological sections that cover in vitro and in vivo methods, including for mtDNA isolation, visualization, deep sequencing, gene editing, and diagnostic aspects of mtDNA disease.
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Base Excision Repair Pathway

Methods and Protocols

Redigeret af Kishor K. Bhakat, Tapas K. Hazra ...

Serier serie Springer Protocols
This detailed volume provides a comprehensive set of experimental protocols and useful strategies to examine the repair of damaged bases via the Base Excision Repair (BER) pathway in vitro and in cells. Beginning with multiple molecular and cellular techniques to examine the excision of damaged bases from double-stranded DNA or DNA wrapped in a nucleosome, the book continues with sections covering ... Læs mere

1.309,23 kr.

This detailed volume provides a comprehensive set of experimental protocols and useful strategies to examine the repair of damaged bases via the Base Excision Repair (BER) pathway in vitro and in cells. Beginning with multiple molecular and cellular techniques to examine the excision of damaged bases from double-stranded DNA or DNA wrapped in a nucleosome, the book continues with sections covering
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Chromosome Analysis

Methods and Protocols

Redigeret af Eisuke Gotoh ...

Serier serie Springer Protocols
This volume provides essential and fundamental protocols on manipulation chromosome. Chapters details methods on the preparation of mitotic chromosome, chromosome aberration, micronucleus (MN), comet assay, karyotyping, Fluorescent in situ hybridization (FISH), premature chromosome condensation (PCC), immunohistochemistry (IHC) staining, new generation sequencing technology and new chromosome ... Læs mere

840,49 kr.

This volume provides essential and fundamental protocols on manipulation chromosome. Chapters details methods on the preparation of mitotic chromosome, chromosome aberration, micronucleus (MN), comet assay, karyotyping, Fluorescent in situ hybridization (FISH), premature chromosome condensation (PCC), immunohistochemistry (IHC) staining, new generation sequencing technology and new chromosome
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Genotype Phenotype Coupling

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Bioinformatics and Functional Genomics

The AGT Cytogenetics Laboratory Manual

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Yeast Surface Display

Phage Display

DNA-Protein Interactions

Mitochondrial DNA

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Chromosome Analysis