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Inositol Pyrophosphates

Methods and Protocols

Redigeret af Adolfo Saiardi, Dorothea Fiedler, Henning J. Jessen ...

Serier serie Springer Protocols
This detailed volume explores methods that reflect the explosion of interest in the important cellular messengers known as inositol pyrophosphates. After a brief historical perspective of the analytical approaches that have contributed to the development of this research field, the book collects methods that cover isolation, detection, and measurement of inositol pyrophosphates, followed by ... Læs mere

1.681,11 kr.

This detailed volume explores methods that reflect the explosion of interest in the important cellular messengers known as inositol pyrophosphates. After a brief historical perspective of the analytical approaches that have contributed to the development of this research field, the book collects methods that cover isolation, detection, and measurement of inositol pyrophosphates, followed by
... Læs mere

Mass Spectrometry-Based Proteomics

Redigeret af Kris Gevaert ...

Serier serie Springer Protocols
This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by ... Læs mere

1.414,11 kr.

This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by
... Læs mere
Protein Tyrosine Phosphatases

Methods and Protocols

Redigeret af Damien Thévenin, Jörg P. Müller ...

Serier serie Springer Protocols
This second edition volume expands on the previous edition with discussions on the latest advancements in protein tyrosine phosphatases (PTP) research used to investigate these essential enzymes and new inhibitors. The new techniques covered in the chapters of this book include studying enzymes in vitro, in cells, and in animal models through proteomics, genomics, and structural biology. ... Læs mere

1.335,49 kr.

This second edition volume expands on the previous edition with discussions on the latest advancements in protein tyrosine phosphatases (PTP) research used to investigate these essential enzymes and new inhibitors. The new techniques covered in the chapters of this book include studying enzymes in vitro, in cells, and in animal models through proteomics, genomics, and structural biology.
... Læs mere
Proximity Labeling

Methods and Protocols

Redigeret af Murat Sunbul, Andres Jäschke ...

Serier serie Springer Protocols
This book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived reactive species to non-specifically label molecules within a certain radius of up to twenty nanometers. Beginning with peroxidase-based proximity ... Læs mere

1.120,66 kr.

This book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived reactive species to non-specifically label molecules within a certain radius of up to twenty nanometers. Beginning with peroxidase-based proximity
... Læs mere
Enzyme-Mediated Ligation Methods

Redigeret af Timo Nuijens, Marcel Schmidt ...

Serier serie Springer Protocols
This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and ... Læs mere

924,61 kr.

This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and
... Læs mere
Peptide Libraries

Methods and Protocols

Redigeret af Hans Michael Maric, Ronald Frank ...

Serier serie Springer Protocols
This volume provides a collection of novel and emerging methods for the generation and application of peptide libraries. Chapter focus on methods and techniques highlighting new avenues for library screening. Written in the Methods in Molecular Biology series format, chapters outline strategies and overview their area or describe specific applications of the method including an introduction, the ... Læs mere

1.867,83 kr.

This volume provides a collection of novel and emerging methods for the generation and application of peptide libraries. Chapter focus on methods and techniques highlighting new avenues for library screening. Written in the Methods in Molecular Biology series format, chapters outline strategies and overview their area or describe specific applications of the method including an introduction, the
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Protein Chromatography

Methods and Protocols

Redigeret af Sinéad T Loughran, John Joseph Milne ...

Serier serie Springer Protocols
This third edition expands on the previous editions with updated and new chapters on protein chromatography. Chapters detail protein stability and storage, avoiding proteolysis, protein quantitation methods, generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins. Written in the format of the highly successful Methods in Molecular Biology ... Læs mere

1.335,49 kr.

This third edition expands on the previous editions with updated and new chapters on protein chromatography. Chapters detail protein stability and storage, avoiding proteolysis, protein quantitation methods, generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins. Written in the format of the highly successful Methods in Molecular Biology
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HDAC/HAT Function Assessment and Inhibitor Development

Methods and Protocols

Redigeret af Oliver H. Krämer ...

Serier serie Springer Protocols
This fully updated edition provides a series of methods for how best to assess functions of histone deacetylases and acetyltransferases. The disease-relevance of dysregulated protein deacetylation by overexpressed or aberrantly activated histone deacetylases has spurred an intense search for novel and improved inhibitors of these enzymes, as reflected in this collection. Expert contributors ... Læs mere

1.587,64 kr.

This fully updated edition provides a series of methods for how best to assess functions of histone deacetylases and acetyltransferases. The disease-relevance of dysregulated protein deacetylation by overexpressed or aberrantly activated histone deacetylases has spurred an intense search for novel and improved inhibitors of these enzymes, as reflected in this collection. Expert contributors
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Lipopolysaccharide Transport

Methods and Protocols

Redigeret af Paola Sperandeo ...

Serier serie Springer Protocols
This volume provides the most widely used protocols and techniques employed in lipopolysaccharide( LPS) transport studies. Chapters detail identification and analysis of essential genes and crucial mutations in LPS transport-related genes, LPS profile determination, LPS chemical analysis, protein-LPS interaction, and LPS visualization. Written in the format of the highly successful Methods in ... Læs mere

933,87 kr.

This volume provides the most widely used protocols and techniques employed in lipopolysaccharide( LPS) transport studies. Chapters detail identification and analysis of essential genes and crucial mutations in LPS transport-related genes, LPS profile determination, LPS chemical analysis, protein-LPS interaction, and LPS visualization. Written in the format of the highly successful Methods in
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Bacterial Regulatory RNA

Methods and Protocols

Redigeret af Véronique Arluison, Claudio Valverde ...

Serier serie Springer Protocols
This second edition details new and updated methods used for studying prokaryotic non-coding RNAs and their protein accomplices. Chapters detail discovery of ncRNAs, characterization of their structure, functions, and their interactomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary ... Læs mere

1.099,74 kr.

This second edition details new and updated methods used for studying prokaryotic non-coding RNAs and their protein accomplices. Chapters detail discovery of ncRNAs, characterization of their structure, functions, and their interactomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary
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Plant Proteostasis

Methods and Protocols

Redigeret af L. Maria Lois, Marco Trujillo ...

Serier serie Springer Protocols
This second edition compiles state-of-art protocols to study the molecular mechanisms that determine proteome composition and contribute to maintaining protein homeostasis. Chapters describe methods that range from the in vitro analysis of enzyme cascades to real-time in vivo studies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their ... Læs mere

1.260,86 kr.

This second edition compiles state-of-art protocols to study the molecular mechanisms that determine proteome composition and contribute to maintaining protein homeostasis. Chapters describe methods that range from the in vitro analysis of enzyme cascades to real-time in vivo studies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their
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Non-Ribosomal Peptide Biosynthesis and Engineering

Methods and Protocols

Redigeret af Michael Burkart, Fumihiro Ishikawa ...

Serier serie Springer Protocols
This volume provides new technologies on NRPSs and related carrier protein dependent synthases, including polyketide synthases (PKS) and fatty acid synthases (FAS). Chapters detail enzymology, structural biology, proteopromics, chemical biology, natural product chemistry, and bioinformatics. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes ... Læs mere

1.176,74 kr.

This volume provides new technologies on NRPSs and related carrier protein dependent synthases, including polyketide synthases (PKS) and fatty acid synthases (FAS). Chapters detail enzymology, structural biology, proteopromics, chemical biology, natural product chemistry, and bioinformatics. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes
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Inositol Pyrophosphates

Personer, der læser dette, syntes også om...

Mass Spectrometry-Based Proteomics

Protein Tyrosine Phosphatases

Proximity Labeling

Enzyme-Mediated Ligation Methods

Peptide Libraries

Protein Chromatography

HDAC/HAT Function Assessment and Inhibitor Development

Lipopolysaccharide Transport

Bacterial Regulatory RNA

Plant Proteostasis

Non-Ribosomal Peptide Biosynthesis and Engineering