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  • Mitochondrial Medicine

    Volume 2: Assessing Mitochondria

    Redigeret af Volkmar Weissig, Marvin Edeas ...
    Serier serie Springer Protocols
    This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume II guides readers through chapters on mitochondrial dysfunction, functional’ mitochondria, mitochondrial retrograde, mitochondrial dNTP pool quantification, mitochondrial ADP-ribosylation, blue-native gel approach, 3D ... Læs mere

    942,61 kr.

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  • Antibody Engineering Volume 1

    Serier serie Springer Protocols
    Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new ... Læs mere

    3.166,13 kr.

  • Drosophila

    Methods and Protocols

    Redigeret af Christian Dahmann ...
    Serier serie Springer Protocols
    This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and ... Læs mere

    1.021,24 kr.

  • Mitochondria

    Methods and Protocols

    Redigeret af Namrata Tomar ...
    Serier serie Springer Protocols
    This volume details comprehensive protocols and methodologies to assess mitochondrial bioenergetics and dynamics in different tissues and cells involving health and pathological states. Chapters guide readers through methods for assessment of the energy metabolism including Oxygen Consumption Rate (OCR), mitochondrial membrane potential, and measuring mitochondrial Ca2+ handling, and ROS emission. ... Læs mere

    1.400,85 kr.

  • Golgi

    Methods and Protocols

    Serier serie Springer Protocols
    This volume provides readers with a collection of new and classical methods, techniques, and applications used to address enduring questions about the structure and functions of the Golgi complex. The chapters in this volume cover diverse topics ranging from model systems; live and fixed cell imaging techniques; in vitro biochemical reconstitution systems; and specific methods developed to study ... Læs mere

    1.756,86 kr.

  • Metabolic Reprogramming

    Methods and Protocols

    Redigeret af Salvatore Papa, Concetta Bubici ...
    Serier serie Springer Protocols
    This volume provides state-of-the-art methods and protocols from researchers in the field of metabolism. Chapters guide readers through methodologies measuring different aspects of lipid metabolism, quantifying the amino acids, mitochondrial and intracellular by-products, detect enzyme and kinase activities, detecting a variety of metabolic pathways, and intracellular networks including nucleotide ... Læs mere

    1.176,74 kr.

  • Peroxisomes

    Methods and Protocols

    Redigeret af Michael Schrader ...
    Serier serie Springer Protocols
    This fully updated volume assembles a comprehensive collection of methods, techniques, and strategies to investigate the molecular and cellular biology of peroxisomes in different organisms. Peroxisome research is on the rise, as novel functions and proteins of this dynamic organelle are still being discovered through studies in model systems including humans, mice, flies, plants, fungi, and yeast ... Læs mere

    1.867,83 kr.

  • HDAC/HAT Function Assessment and Inhibitor Development

    Methods and Protocols

    Redigeret af Oliver H. Krämer ...
    Serier serie Springer Protocols
    This fully updated edition provides a series of methods for how best to assess functions of histone deacetylases and acetyltransferases. The disease-relevance of dysregulated protein deacetylation by overexpressed or aberrantly activated histone deacetylases has spurred an intense search for novel and improved inhibitors of these enzymes, as reflected in this collection. Expert contributors ... Læs mere

    1.587,64 kr.

  • Membrane Trafficking

    Methods and Protocols

    Redigeret af Jingshi Shen ...
    Serier serie Springer Protocols
    This detailed volume presents a series of methods exploring membrane trafficking research, ranging from genetics and high-resolution imaging to in vitro biochemical and biophysical assays. Covering virtually all the major trafficking branches, the book delves into the exocytic pathway, which focuses on cargo transport from the ER to the Golgi, through the Golgi cisternae, and to the plasma ... Læs mere

    1.260,86 kr.

  • Lipopolysaccharide Transport

    Methods and Protocols

    Redigeret af Paola Sperandeo ...
    Serier serie Springer Protocols
    This volume provides the most widely used protocols and techniques employed in lipopolysaccharide( LPS) transport studies. Chapters detail identification and analysis of essential genes and crucial mutations in LPS transport-related genes, LPS profile determination, LPS chemical analysis, protein-LPS interaction, and LPS visualization. Written in the format of the highly successful Methods in ... Læs mere

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  • Single-Domain Antibodies

    Methods and Protocols

    Redigeret af Greg Hussack, Kevin A. Henry ...
    Serier serie Springer Protocols
    This volume covers current and emerging techniques for studying single-domain antibodies (sdAbs). Chapters guide readers through the biology and immunology of sdAbs in camelids and sharks, isolation of sdAbs, protein engineering approaches to optimize the solubility, stability, valency and antigen binding affinity of sdAbs, and specialized applications of sdAbs. Written in the format of the highly ... Læs mere

    1.681,11 kr.

  • RNA-Protein Complexes and Interactions

    Methods and Protocols

    Redigeret af Ren-Jang Lin ...
    Serier serie Springer Protocols
    This second edition updates, complements, and expands upon the first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. Chapters explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. Written in ... Læs mere

    1.756,86 kr.