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  • Brain Tumor Stem Cells

    Methods and Protocols

    Redigeret af Sheila K. Singh, Chitra Venugopal ...
    Serier serie Springer Protocols
    This updated volume explores the technically challenging study of brain tumor stem cells (BTSCs) with their unique capacity to self-renew, proliferate, and initiate tumor formation. The book collects up-to-date methodologies to isolate and propagate BTSCs and to study them using various cutting-edge techniques, in service of bringing us closer to translating the discoveries made from these cells ... Læs mere

    1.587,64 kr.

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  • The AGT Cytogenetics Laboratory Manual

    Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics.Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis ... Læs mere

    1.471,24 kr.

  • Micro- and Nanosystems for Biotechnology

    Serier serie Advanced Biotechnology
    Emphasizing their emerging capabilities, this volume provides a strong foundation for an understanding of how micro- and nanotechnologies used in biomedical research have evolved from concepts to working platforms.Volume editor Christopher Love has assembled here a highly interdisciplinary group of authors with backgrounds ranging from chemical engineering right up to materials science to reflect ... Læs mere

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  • Zebrafish

    Methods and Protocols

    Serier serie Springer Protocols
    This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the ... Læs mere

    1.641,99 kr.

  • Cancer Cell Biology

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    Redigeret af Sherri L. Christian ...
    Serier serie Springer Protocols
    This volume provides detailed methods on the mechanisms of underlying cancer cell biology. Chapters guide readers through techniques for culturing cancer cell lines, xenografts, cryopreservation of tumor cells, analyzing the co-culture of breast cancer cells, protein secretion by ELISA, flow cytometry-based, multi-parametric immunofluorescence analysis, protein expression by western blot, analysis ... Læs mere

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  • B-Cell Receptor Signaling

    Methods and Protocols

    Redigeret af Palash Chandra Maity ...
    Serier serie Springer Protocols
    B-lymphocytes produce antibodies, mediate humoral responses, and act as a key to the adaptive immune system of our body. The development and function of B cells are largely dependent on B cell antigen receptors (BCRs), which transmit a multitude of signals upon binding to the cognate antigen and activate the survival, proliferation, and differentiation of B cells thereof. Thus, studying the BCR ... Læs mere

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  • The P2X7 Receptor

    Methods and Protocols

    Redigeret af Annette Nicke ...
    Serier serie Springer Protocols
    This detailed volume covers diverse aspects of P2X7 receptor analysis, ranging from its molecular structure to related pharmacological and immunological tools, via its analysis in heterologous expression systems as well as assays using primary cells and whole animal models. After three introductory chapters that focus on its structure, ligands, and physiological functions, the book details the ... Læs mere

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  • Immunoproteomics

    Methods and Protocols

    Redigeret af Kelly M. Fulton, Susan M. Twine ...
    Serier serie Springer Protocols
    This third edition volume expands on the previous editions with new chapters discussing advancements in mass spectrometry resolution, sensitivity, and speed, including the refinement of the identification of peptides presented on the surface of major histocompatibility proteins. These technologies continue to advance vaccine development, as well as cancer immunotherapy and precision medicine. ... Læs mere

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  • Regulatory T-Cells

    Methods and Protocols

    Redigeret af Masahiro Ono ...
    Serier serie Springer Protocols
    This volume provides methods and models for investigating the immunosuppressive subset of CD4+ T-cells, regulatory T-cells (Treg). Chapters guide readers through, basic protocols for analyzing Treg in mice and humans, single-cell analysis for analyzing Treg, and methods for the analysis of Treg in clinical application. Written in the format of the highly successful Methods in Molecular Biology ... Læs mere

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  • T Cell Activation

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    Redigeret af Björn-Philipp Diercks ...
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    This volume provides detailed, up-to-date methods used in basic and applied research on T-cell activation. Chapters explore the fundamentals in T-cell biology a board range cutting-edge techniques from initial Ca2+ live-cell imaging to downstream effector functions in autoimmune disease. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an ... Læs mere

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  • Autoimmunity

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    Redigeret af Rami Bechara ...
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    This volume brings together a comprehensive and up-to-date collection of protocols that reflect the diverse experimental strategies. Chapters detail T-cell, macrophage characterization, neutrophil functional assays, organoid culture methods, spatial transcriptomics, RNA FISH, microRNA profiling, and ribosome profiling. Written in the highly successful Methods in Molecular Biology series format, ... Læs mere

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  • Dendritic Cells

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    Redigeret af Vanja Sisirak ...
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    This detailed volume provides methods that can be used to study dendritic cell (DC) ontogeny, isolation, migration, and functions. After an introduction to murine and human DC subsets and their unique transcriptional, phenotypic, and functional properties, the book continues with sections covering in vivo studies, in vitro differentiation, enrichment, functional characterization, as well as Omics ... Læs mere

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