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  • Baculovirus

    Methods and Protocols

    Redigeret af Manon M.J. Cox ...
    Serier serie Springer Protocols
    This detailed volume provides up-to-date guidance on techniques used to work with baculoviruses and insect cells. The book provides basic methods to create recombinant baculoviruses, to improve productivity, to produce a variety of products, to purify products, to quantify baculovirus stocks or to quantify product produced, and it concludes with alternative uses of either baculovirus or insect ... Læs mere

    1.176,74 kr.

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  • Culture of Animal Cells

    A Manual of Basic Technique and Specialized Applications

    Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made – particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in ... Læs mere

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  • The AGT Cytogenetics Laboratory Manual

    Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics.Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis ... Læs mere

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  • Zebrafish

    Methods and Protocols

    Serier serie Springer Protocols
    This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the ... Læs mere

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  • Laboratory Protocols in Fungal Biology

    Current Methods in Fungal Biology

    Serier serie Biomedical and Life Sciences (R0)
    Laboratory Protocols in Fungal Biology presents the latest techniques in fungal biology. This book analyzes information derived through real experiments, and focuses on cutting edge techniques in the field. The book comprises 57 chapters contributed from internationally recognised scientists and researchers. Experts in the field have provided up-to-date protocols covering a range of frequently ... Læs mere

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  • Genome Editing in Animals

    Methods and Protocols

    Redigeret af Izuho Hatada ...
    Serier serie Springer Protocols
    This second edition provides new and updated protocols that can be used for generation of knockout animals. Chapters guide the reader through basic protocols for three genome editing technologies, target design tools, and specific protocols for each animal. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the ... Læs mere

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  • Plant-Virus Interactions

    Serier serie Springer Protocols
    This detailed volume provides practical guidance on techniques in plant-virus interaction research, from targeting specific molecular interactions within the virus-host interactome to the identification of the complete virus-host protein-protein interaction network. After chapters on acquiring the necessary molecular tools, the book continues with biochemical and genetic approaches to confirming ... Læs mere

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  • Recombinant Protein Expression in Mammalian Cells

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    Redigeret af David L. Hacker ...
    Serier serie Springer Protocols
    This fully updated volume explores notable developments in the field of mammalian cell-based recombinant protein production. Beginning with methods for transient recombinant protein production, the book continues with methods for stable cell pool generation, protein production using stable clonal cell lines, as well as high-throughput screening technologies for characterizing transient cell ... Læs mere

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  • Mammalian Oocyte Development

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    Serier serie Springer Protocols
    This volume details various aspects of the very final stages of mouse oocyte development, and very early embryo development. Chapters present methods ranging from in vitro growth of follicles, ewe oocytes, meiosis, identification of LADs (Lamin Associated Domains)/TADs (Topological Associated Domains), analysis of the oocyte, early embryo transcriptome, and mechanical characterization of these ... Læs mere

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  • Trypanosomatids

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    This volume explores the latest methods used by researchers to study different trypanosomatid parasites. These methods cover numerous disciplines, from organismal biology to molecular mechanism. The chapters in this book cover topics such as high-throughput sequencing; next-generation analysis of trypanosomatid genome stability and instability; DNA repair in cell extracts; ribosome profiling; and ... Læs mere

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  • Recombinant Glycoproteins

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    Redigeret af Steven B. Bradfute ...
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    This detailed volume explores the production and purification of glycoproteins for therapeutic use or basic research, as well as the necessary in-depth understanding of cellular and acellular systems available for these purposes, their advantages and disadvantages, and considerations for choosing the most appropriate system for the desired application. Beginning with chapters on viral-encoded ... Læs mere

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  • Single-Domain Antibodies

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    Redigeret af Greg Hussack, Kevin A. Henry ...
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    This volume covers current and emerging techniques for studying single-domain antibodies (sdAbs). Chapters guide readers through the biology and immunology of sdAbs in camelids and sharks, isolation of sdAbs, protein engineering approaches to optimize the solubility, stability, valency and antigen binding affinity of sdAbs, and specialized applications of sdAbs. Written in the format of the highly ... Læs mere

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