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  • Muscle Stem Cells

    Methods and Protocols

    Redigeret af Nathalie Didier, Caroline E. Brun ...
    Serier serie Springer Protocols
    This detailed volume provides an experimental roadmap for interrogating muscle stem cell biology, from tissue dissociation and cell isolation to ex vivo analysis, in vitro modeling, functional assays, and multi-omics technologies. The book explores how to capture or preserve the physiological state of muscle stem cells in situ and ex vivo, techniques for in situ imaging, computational analysis of ... Læs mere

    1.868,84 kr.

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  • Zebrafish

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    This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the ... Læs mere

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  • Mass Spectrometry-Based Proteomics

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    This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by ... Læs mere

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  • Practical Book in Experimantal Pharmacology and Biochemical Techniques

    This book provides concise procedures for a range of practical experiments in pharmaceutical analysis, pharmacology, and molecular biology. All procedures must be performed in accordance with Good Laboratory Practices (GLP), under the supervision of qualified personnel, and with adherence to all safety protocols. ... Læs mere

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  • Autoimmunity

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    This volume brings together a comprehensive and up-to-date collection of protocols that reflect the diverse experimental strategies. Chapters detail T-cell, macrophage characterization, neutrophil functional assays, organoid culture methods, spatial transcriptomics, RNA FISH, microRNA profiling, and ribosome profiling. Written in the highly successful Methods in Molecular Biology series format, ... Læs mere

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  • Cancer Cell Biology

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    Redigeret af Sherri L. Christian ...
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    This volume provides detailed methods on the mechanisms of underlying cancer cell biology. Chapters guide readers through techniques for culturing cancer cell lines, xenografts, cryopreservation of tumor cells, analyzing the co-culture of breast cancer cells, protein secretion by ELISA, flow cytometry-based, multi-parametric immunofluorescence analysis, protein expression by western blot, analysis ... Læs mere

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  • Germ Cell Development

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    Redigeret af Marco Barchi, Massimo De Felici ...
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    This detailed volume explores techniques for the isolation, purification, and establishment of in vitro germ cell (GS) systems at different stages of development in a variety of organisms, including chickens, mice, rats, and humans. The book additionally describes cutting-edge analytic and informatic tools to study germ cell development at the single cell level and meiotic recombination. Written ... Læs mere

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  • Skeletal Muscle Stem Cells

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    Redigeret af Atsushi Asakura ...
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    This volume looks at the latest technologies and methods--combined with new genetic tools available in animal models--used in this constantly evolving field. The chapters in this book are organized into three sections: Section one covers muscle stem cells and progenitor cells; Section Two discusses animal models for muscle stem cells and regeneration; and Section Three explores bioinformatics and ... Læs mere

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    This second edition provides a comprehensive collection of classic and cutting-edge methodologies as well as bioinformatics and genome-editing approaches for cancer cells and tissues. Chapters beginning with the Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step ... Læs mere

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  • Myogenesis and Skeletal Muscle Biology

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    This volume offers an extensive and forward-looking toolkit for the study of myogenesis and skeletal muscle biology, spanning stem cell isolation, tissue engineering, advanced imaging, and functional analysis. The book covers state-of-the-art methods for the isolation, expansion, and differentiation of skeletal muscle satellite cells and human induced pluripotent stem cells, including innovative ... Læs mere

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    B-lymphocytes produce antibodies, mediate humoral responses, and act as a key to the adaptive immune system of our body. The development and function of B cells are largely dependent on B cell antigen receptors (BCRs), which transmit a multitude of signals upon binding to the cognate antigen and activate the survival, proliferation, and differentiation of B cells thereof. Thus, studying the BCR ... Læs mere

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