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Genotyping

Methods and Protocols

Edición de Anthony Torres ...

Series series Springer Protocols
This volume covers the latest techniques used to study single nucleotide polymorphisms (SNP) genotyping, such as Sanger sequencing, qPCR, Nanopore sequencing, and PACE. The chapters in this book cover topics such as SNP nomenclature, databases, and significance of SNPs in various fields; PCR-based techniques like HRM and CD-PCR; isothermal amplification methodologies; high-throughput methods like ... Leer más

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This volume covers the latest techniques used to study single nucleotide polymorphisms (SNP) genotyping, such as Sanger sequencing, qPCR, Nanopore sequencing, and PACE. The chapters in this book cover topics such as SNP nomenclature, databases, and significance of SNPs in various fields; PCR-based techniques like HRM and CD-PCR; isothermal amplification methodologies; high-throughput methods like
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PCR Guru

An Ultimate Benchtop Reference for Molecular Biologists

de Ayaz Najafov, Gerta Hoxhaj ...

PCR Guru: An Ultimate Benchtop Reference for Molecular Biologists is provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with PCR setup and optimization. As a laboratory guide, it emphasizes the technical aspects of employing PCR as a tool in molecular biology laboratories. The book covers the history of PCR and the basic ... Leer más

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PCR Guru: An Ultimate Benchtop Reference for Molecular Biologists is provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with PCR setup and optimization. As a laboratory guide, it emphasizes the technical aspects of employing PCR as a tool in molecular biology laboratories. The book covers the history of PCR and the basic
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Drosophila

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Edición de Christian Dahmann ...

Series series Springer Protocols
This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and ... Leer más

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This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and
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Catharanthus roseus

Methods and Protocols

Edición de Vincent Courdavault, Sebastien Besseau ...

Series series Springer Protocols
This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in C. roseus from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, transcription factor characterization up to functional genomic tools based on gene overexpression. Written in the format of the highly successful Methods in ... Leer más

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This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in C. roseus from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, transcription factor characterization up to functional genomic tools based on gene overexpression. Written in the format of the highly successful Methods in
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Understanding PCR

A Practical Bench-Top Guide

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Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips. - Includes resources for ... Leer más

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Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips. - Includes resources for
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Mass Spectrometry-Based Proteomics

Edición de Kris Gevaert ...

Series series Springer Protocols
This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by ... Leer más

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This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by
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Clinical Applications of Nucleic Acid Amplification

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Series series Springer Protocols
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This volume details applications, methods, and protocols on nucleic acid amplification. Chapters detail the management of organ and stem cell transplantation, constitutional genetics and genomics, epidemiology, public health, nucleic acid detection, methods for amplification and sequencing, nuclease-assisted mutant allele enrichment, isothermal, and high-throughput sequencing. Written in the
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Series series Springer Protocols
This detailed volume explores techniques utilizing the fission yeast Schizosaccharomyces pombe, a powerful tractable model system for eukaryotic biology. The book begins with a section on microscopy and imaging, and it continues with chapters covering genome and plasmid editing, including the use of CRISPR/Cas9 technologies, as well as the analysis of DNA damage, repair, and recombination, and ... Leer más

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This detailed volume explores techniques utilizing the fission yeast Schizosaccharomyces pombe, a powerful tractable model system for eukaryotic biology. The book begins with a section on microscopy and imaging, and it continues with chapters covering genome and plasmid editing, including the use of CRISPR/Cas9 technologies, as well as the analysis of DNA damage, repair, and recombination, and
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Euglenozoa

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Edición de Paul A.M. Michels, Michael L. Ginger, Anna Karnkowska, Laura-Isobel McCall, Ariel M. Silber ...

Series series Springer Protocols
This detailed collection covers methods recently developed for experiments on organisms from each of the major euglenozoan groups, including diplonemids, euglenids, and kinetoplastids. Volume 1 presents a historical review of the research into euglenozoans, followed by sections of techniques involving isolation and culturing of different species of euglenozoans, as well as a variety of -omics ... Leer más

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This detailed collection covers methods recently developed for experiments on organisms from each of the major euglenozoan groups, including diplonemids, euglenids, and kinetoplastids. Volume 1 presents a historical review of the research into euglenozoans, followed by sections of techniques involving isolation and culturing of different species of euglenozoans, as well as a variety of -omics
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Digital PCR

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Series series Springer Protocols
This detailed volume explores digital polymerase chain reaction (dPCR), a third generation PCR technology that enables absolute quantification by counting positive and negative partitions after endpoint amplification. The book aims to provide an overview of the various possibilities currently available, both in terms of instrumentation and applications. The selected protocols cover a wide range of ... Leer más

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This detailed volume explores digital polymerase chain reaction (dPCR), a third generation PCR technology that enables absolute quantification by counting positive and negative partitions after endpoint amplification. The book aims to provide an overview of the various possibilities currently available, both in terms of instrumentation and applications. The selected protocols cover a wide range of
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Meiosis

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This volume details protocols on meiosis, covering the latest technological and methodological advances in this research field. Chapters guide readers through methods on genomics, biochemistry, super-resolution microscopy, traditional genetics, cytological methods, as well as machine learning and in silico modelling. Written in the highly successful Methods in Molecular Biology series format,
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Meiosis