Optical probes, particularly the fluorescent varieties, enable researchers to observe cellular events in real time and with great spatial resolution. Optical Probes in Biology explores the diverse capabilities of these powerful and versatile tools and presents various approaches used to design, develop, and implement them. The book examines the use

This book introduces and discusses the latest in biomedical research--Pharmaceutical Nanotechnology applied at the sub-cellular level.

With extensive coverage of synthesis techniques and applications, this text describes chemical biology techniques which have gained significant impetus during the last five years. It focuses on the methods for obtaining modified and native nucleic acids, and their biological applications. Topics covered include:chemical synthesis of modified RNAexpansion of the genetic alphabet in nucleic acids by creating new base pairschemical biology of DNA replication:...

Sheds new light on intrinsically disordered proteins and peptides, including their role in neurodegenerative diseasesWith the discovery of intrinsically disordered proteins and peptides (IDPs), researchers realized that proteins do not necessarily adopt a well defined secondary and tertiary structure in order to perform biological functions. In fact, IDPs play biologically relevant roles, acting as inhibitors, scavengers, and even facilitating DNA/RNA-protein interactions. Due to t...

Summing up almost a decade of biomedical research, this topical and eagerly awaited handbook is the first reference on the topic to incorporate recent breakthroughs in amyloid research.The first part covers the structural biology of amyloid fibrils and pre-fibrillar assemblies, including a description of current models for amyloid formation. The second part looks at the diagnosis and biomedical study of amyloid in humans and in animal models, while the final section discusses pharm...

This book describes the events of primary energy transduction in life processes. Life as we know it depends on pumping protons across membranes. New tools to study the protein complexes involved has led to recent intensified progress in the field.Primary Energy Transduction in Biology focusses on recent structural results and new biophysical insights. These have been made possible by recent advances in high-resolution protein structures, in physical techniques to study reactions in ...

Molecular Analysis and Genome Discovery, Second Edition is a completely revised and updated new edition of this successful book. The text provides a comprehensive overview of recent developments in the fast moving field of molecular based diagnostics of disease markers. Key concepts and applications are provided alongside practical information on current techniques currently being researched and developed. Each chapter offers an up-to-date analysis of the subject encompassing the ...

This volume of Progress in Molecular Biology and Translational Science introduces emerging strategies of studying RNA structure and function using nanotechnological tools. The volume aims to provide the readers with a novel view and give them opportunities to think about how to incorporate the new technologies into their own research. - This book informs and updates on strategies to take advantages of nanotechnological tools to answer to fundamental questions in RNA science - Collection of...

New Methods and Sensors for Membrane and Cell Volume Research, Volume 88 provides an overview of novel experimental approaches to study both the cell membrane and the under-membrane space – the cytosol, which have lately began drawing renewed attention. The book's overall emphasis is on fluorescent and FRET-based sensors, however, other optical (such as variants of transmission microscopy) and non-optical methods (neutron scattering and mass spectrometry) also have dedicated chapters. This...

Optogenetic tools have allowed significant advances in the understanding of biological problems, particularly in the neurosciences field. Biological tools as well as optical set-ups have evolved and a wide range of probes and light-controllable modules are now available.The aim of this book is to give a flavour of illumination strategies and imaging with an overview of the different optogenetic tools and their main applications in cell biology. Based on examples covering the differe...

One of the major challenges of modern biology and medicine consists in finding means to visualize biomolecules in their natural environment with the greatest level of accuracy, so as to gain insight into their properties and behaviour in a physiological and pathological setting. This has been achieved thanks to the design of novel imaging agents, in particular to fluorescent biosensors. Fluorescence Biosensors comprise a large set of tools which are useful for fundamental purposes as well ...

Ion Channels Part A, Volume 651 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of new developments on the topic. Each chapter is written by an international board of authors. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series