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  • Deubiquitinases

    Methods and Protocols

    Serier serie Springer Protocols
    This book aims to advance the understanding of deubiquitinases (DUBs) and DUB-like enzymes. Chapters detail methods used to identify, classify,and biochemically characterize DUBs along with approaches that enable both the determination and alteration of DUB biological function.. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an ... Læs mere

    1.400,85 kr.

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  • Antibody Engineering Volume 1

    Serier serie Springer Protocols
    Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new ... Læs mere

    3.166,13 kr.

  • Catharanthus roseus

    Methods and Protocols

    Serier serie Springer Protocols
    This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in C. roseus from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, transcription factor characterization up to functional genomic tools based on gene overexpression. Written in the format of the highly successful Methods in ... Læs mere

    1.867,83 kr.

  • Enzyme-Mediated Ligation Methods

    Redigeret af Timo Nuijens, Marcel Schmidt ...
    Serier serie Springer Protocols
    This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and ... Læs mere

    924,61 kr.

  • Plant Proteostasis

    Methods and Protocols

    Redigeret af L. Maria Lois, Marco Trujillo ...
    Serier serie Springer Protocols
    This second edition compiles state-of-art protocols to study the molecular mechanisms that determine proteome composition and contribute to maintaining protein homeostasis. Chapters describe methods that range from the in vitro analysis of enzyme cascades to real-time in vivo studies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their ... Læs mere

    1.260,86 kr.

  • Mass Spectrometry-Based Proteomics

    Redigeret af Kris Gevaert ...
    Serier serie Springer Protocols
    This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by ... Læs mere

    1.414,11 kr.

  • Protein Chromatography

    Methods and Protocols

    Serier serie Springer Protocols
    This third edition expands on the previous editions with updated and new chapters on protein chromatography. Chapters detail protein stability and storage, avoiding proteolysis, protein quantitation methods, generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins. Written in the format of the highly successful Methods in Molecular Biology ... Læs mere

    1.335,49 kr.

  • The Ubiquitin Code

    Redigeret af Manuel S Rodriguez, Rosa Barrio ...
    Serier serie Springer Protocols
    This volume details novel and popular approaches to decipher the Ubiquitin Code. Chapters guide readers through ubiquitin chain complexity, methods to generate non-hydrolysable ubiquitin-NEDD8, a fully synthetic method for the linear synthesis of fluorescently labelled rhodamine LC3A and LC3B, a HECT ligase-mediated ubiquitylation in vitro, a RNAi-based screening to identify DUBs, a Yuh1 activity ... Læs mere

    1.008,61 kr.

  • Non-Ribosomal Peptide Biosynthesis and Engineering

    Methods and Protocols

    Serier serie Springer Protocols
    This volume provides new technologies on NRPSs and related carrier protein dependent synthases, including polyketide synthases (PKS) and fatty acid synthases (FAS). Chapters detail enzymology, structural biology, proteopromics, chemical biology, natural product chemistry, and bioinformatics. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes ... Læs mere

    1.176,74 kr.

  • RNA-Protein Complexes and Interactions

    Methods and Protocols

    Redigeret af Ren-Jang Lin ...
    Serier serie Springer Protocols
    This second edition updates, complements, and expands upon the first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. Chapters explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. Written in ... Læs mere

    1.756,86 kr.

  • Lipopolysaccharide Transport

    Methods and Protocols

    Redigeret af Paola Sperandeo ...
    Serier serie Springer Protocols
    This volume provides the most widely used protocols and techniques employed in lipopolysaccharide( LPS) transport studies. Chapters detail identification and analysis of essential genes and crucial mutations in LPS transport-related genes, LPS profile determination, LPS chemical analysis, protein-LPS interaction, and LPS visualization. Written in the format of the highly successful Methods in ... Læs mere

    933,87 kr.

  • Genotype Phenotype Coupling

    Methods and Protocols

    Redigeret af Stefan Zielonka, Simon Krah ...
    Serier serie Springer Protocols
    This detailed new edition broadens the scope of the first edition by moving beyond classical display technologies. This book explores methodologies for the generation of natively paired antibody libraries, single cell technologies, alternative scaffolds, and in silico antibody sequence assessments are described. The application of these methods may allow for a generation of improved therapeutics ... Læs mere

    1.428,99 kr.