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  • RNA-Protein Complexes and Interactions

    Methods and Protocols

    Redigeret af Ren-Jang Lin ...
    Serier serie Springer Protocols
    This second edition updates, complements, and expands upon the first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. Chapters explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. Written in ... Læs mere

    1.756,86 kr.

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  • Antibody Engineering Volume 1

    Serier serie Springer Protocols
    Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new ... Læs mere

    3.166,13 kr.

  • Alternative pre-mRNA Splicing

    Theory and Protocols

    This book was written for graduate and medical students, as well as clinicians and postdoctoral researchers. It describes the theory of alternative pre-mRNA splicing intwelve introductory chapters and then introduces protocols and their theoretical background relevant for experimental research. These 43 practical chapters cover: Basic methods, Detection of splicing events, Analysis of alternative ... Læs mere

    639,24 kr.

  • Drosophila

    Methods and Protocols

    Redigeret af Christian Dahmann ...
    Serier serie Springer Protocols
    This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and ... Læs mere

    1.021,24 kr.

  • The Mitoribosome

    Methods and Protocols

    Serier serie Springer Protocols
    This volume provides classic and new methods to study the structure, assembly pathway, and protein synthesis ability of mitoribosomes across species. Following an introduction of fundamental concepts on the topic, method chapters present detailed protocols based on cryo-electron tomography, cryo-EM approaches, mitoribosome purification techniques, mitochondrial translation assays, and methods to ... Læs mere

    1.951,98 kr.

  • Genetically Incorporated Non-Canonical Amino Acids

    Methods and Protocols

    Redigeret af Yu-Hsuan Tsai, Simon J. Elsässer ...
    Serier serie Springer Protocols
    This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification. ... Læs mere

    1.092,74 kr.

  • Protein Arginylation

    Methods and Protocols

    Redigeret af Anna S. Kashina ...
    Serier serie Springer Protocols
    This second edition provides updated and new chapters that detail comprehensive overview of all the existing methods on analyzing protein arginylation. Chapters guide readers through the early methods utilizing crude protein preparations and whole-cell assays to the latest advanced methods involving recombinant protein techniques, antibodies, high precision mass spectrometry, and chemical probes. ... Læs mere

    1.092,74 kr.

  • Enzyme-Mediated Ligation Methods

    Redigeret af Timo Nuijens, Marcel Schmidt ...
    Serier serie Springer Protocols
    This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and ... Læs mere

    924,61 kr.

  • Spatial Genome Organization

    Methods and Protocols

    Redigeret af Tom Sexton ...
    Serier serie Springer Protocols
    This detailed volume explores a variety of cutting-edge techniques used to interrogate spatial genome organization. Beginning with a section covering the vital chromosome conformation capture (3C) technique, this collection continues with chapters on targeted Hi-C approaches, sequencing-based approaches to assess nuclear environment, as well as single-cell technologies to better characterize the ... Læs mere

    1.400,85 kr.

  • Heterologous Expression of Membrane Proteins

    Methods and Protocols

    Redigeret af Isabelle Mus-Veteau ...
    Serier serie Springer Protocols
    This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus ... Læs mere

    1.400,85 kr.

  • Poly(ADP-Ribose) Polymerase

    Methods and Protocols

    Redigeret af Alexei V. Tulin ...
    Serier serie Springer Protocols
    This detailed volume explores poly(ADP-ribose) polymerases (PARPs) in the biology of eukaryotes and their relevance to human health. Beginning with a section on the detection and quantification of poly(ADP-ribose) polymer (pADPr), the book continues by delving into the identification of protein targets, functional analysis, the poly(ADP-ribosyl)ating pathway in chromatin and genes expression, as ... Læs mere

    1.260,86 kr.

  • Non-Ribosomal Peptide Biosynthesis and Engineering

    Methods and Protocols

    Serier serie Springer Protocols
    This volume provides new technologies on NRPSs and related carrier protein dependent synthases, including polyketide synthases (PKS) and fatty acid synthases (FAS). Chapters detail enzymology, structural biology, proteopromics, chemical biology, natural product chemistry, and bioinformatics. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes ... Læs mere

    1.176,74 kr.