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  • Protein Arginylation

    Methods and Protocols

    Redigeret af Anna S. Kashina ...
    Serier serie Springer Protocols
    This second edition provides updated and new chapters that detail comprehensive overview of all the existing methods on analyzing protein arginylation. Chapters guide readers through the early methods utilizing crude protein preparations and whole-cell assays to the latest advanced methods involving recombinant protein techniques, antibodies, high precision mass spectrometry, and chemical probes. ... Læs mere

    1.092,74 kr.

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  • Antibody Engineering Volume 1

    Serier serie Springer Protocols
    Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new ... Læs mere

    3.166,13 kr.

  • Peptides

    Chemistry and Biology

    Thoroughly updated, incorporating around 25 % new material, Sewald/Jakubke remains the only modern and scientifically up-to-date advanced textbook on peptide biochemistry, distilling the knowledge of hundreds of publications into a highly readable synopsis of this diverse field.The authors explain the broad fundamentals of peptide synthesis and structure, systematically addressing important ... Læs mere

    1.189,36 kr.

  • Enzymatic Analysis

    A Practical Guide

    Serier serie Biological Methods
    Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refinement of old ones, and to the adaptation in general of methods to almost any scale of sensitivity. ... Læs mere

    1.681,11 kr.

  • Alternative pre-mRNA Splicing

    Theory and Protocols

    This book was written for graduate and medical students, as well as clinicians and postdoctoral researchers. It describes the theory of alternative pre-mRNA splicing intwelve introductory chapters and then introduces protocols and their theoretical background relevant for experimental research. These 43 practical chapters cover: Basic methods, Detection of splicing events, Analysis of alternative ... Læs mere

    639,24 kr.

  • RNA-Protein Complexes and Interactions

    Methods and Protocols

    Redigeret af Ren-Jang Lin ...
    Serier serie Springer Protocols
    This second edition updates, complements, and expands upon the first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. Chapters explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. Written in ... Læs mere

    1.756,86 kr.

  • Genetically Incorporated Non-Canonical Amino Acids

    Methods and Protocols

    Redigeret af Yu-Hsuan Tsai, Simon J. Elsässer ...
    Serier serie Springer Protocols
    This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification. ... Læs mere

    1.092,74 kr.

  • The Mitoribosome

    Methods and Protocols

    Serier serie Springer Protocols
    This volume provides classic and new methods to study the structure, assembly pathway, and protein synthesis ability of mitoribosomes across species. Following an introduction of fundamental concepts on the topic, method chapters present detailed protocols based on cryo-electron tomography, cryo-EM approaches, mitoribosome purification techniques, mitochondrial translation assays, and methods to ... Læs mere

    1.951,98 kr.

  • Chaperones

    Methods and Protocols

    Serier serie Springer Protocols
    This second edition volume expands on the previous edition with new discussions on the latest techniques used to study molecular chaperones and the stress response. The chapters in this book cover such as analysis of the initiation and regulation of the stress response; the role of heat shock protein 90 (Hsp90) in gene expression through chromosome-immunoprecipitation; features of chaperone ... Læs mere

    1.571,11 kr.

  • Enzyme-Mediated Ligation Methods

    Redigeret af Timo Nuijens, Marcel Schmidt ...
    Serier serie Springer Protocols
    This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and ... Læs mere

    924,61 kr.

  • Phage Display

    Methods and Protocols

    Redigeret af Michael Hust, Theam Soon Lim ...
    Serier serie Springer Protocols
    This second edition details new and updated methods on different antibody libraries, along with novel approaches for antibody discovery. Chapters focuses on the construction of antibody libraries, antibody expression, complementary approaches for antibody selection, and other phage display related applications. Written in the highly successful Methods in Molecular Biology series format, chapters ... Læs mere

    2.045,38 kr.

  • HDAC/HAT Function Assessment and Inhibitor Development

    Methods and Protocols

    Redigeret af Oliver H. Krämer ...
    Serier serie Springer Protocols
    This fully updated edition provides a series of methods for how best to assess functions of histone deacetylases and acetyltransferases. The disease-relevance of dysregulated protein deacetylation by overexpressed or aberrantly activated histone deacetylases has spurred an intense search for novel and improved inhibitors of these enzymes, as reflected in this collection. Expert contributors ... Læs mere

    1.587,64 kr.