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  • RNA Amplification and Analysis

    Methods and Protocols

    Redigeret af Mekbib Astatke ...
    Serier serie Springer Protocols
    This volume provides methods to analyze RNA and gain insight to a number of critical cellular functions. Chapters detail RNA isolation and enrichment, RNA amplification and detection, RNA sequencing, analysis of RNA modification, RNA delivery approaches, and analysis of RNA binding and catalytic activity. Written in the highly successful Methods in Molecular Biology series format, chapters include ... Læs mere

    1.176,74 kr.

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  • Mass Spectrometry-Based Proteomics

    Redigeret af Kris Gevaert ...
    Serier serie Springer Protocols
    This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by ... Læs mere

    1.414,11 kr.

  • Advanced Methods in Structural Biology

    Redigeret af Ângela Sousa, Luis Passarinha ...
    Serier serie Springer Protocols
    This volume explores the latest integrated bioprocesses and technologies used to study the production of the target recombinant protein of therapeutic or diagnostic interest; its isolation, purification, and stabilization; and the bio-interaction and structural analyses. The chapters in this book are organized into four parts. Part One covers production methods of soluble and membrane proteins in ... Læs mere

    1.396,46 kr.

  • Bacterial Regulatory RNA

    Methods and Protocols

    Serier serie Springer Protocols
    This second edition details new and updated methods used for studying prokaryotic non-coding RNAs and their protein accomplices. Chapters detail discovery of ncRNAs, characterization of their structure, functions, and their interactomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary ... Læs mere

    1.099,74 kr.

  • Intracellular Lipid Transport

    Methods and Protocols

    Redigeret af Guillaume Drin ...
    Serier serie Springer Protocols
    This fully updated volume explores different classes of lipid transporters, such as lipid transfer proteins, flippases, and scramblases, and significant advances in solving their biological functions. The first half of the book features methodologies to measure the movement of lipids between or in organelle membranes inside eukaryotic cells, including plant cells, or in prokaryotic cells, and the ... Læs mere

    2.325,57 kr.

  • Mitochondrial Medicine

    Volume 2: Assessing Mitochondria

    Redigeret af Volkmar Weissig, Marvin Edeas ...
    Serier serie Springer Protocols
    This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume II guides readers through chapters on mitochondrial dysfunction, functional’ mitochondria, mitochondrial retrograde, mitochondrial dNTP pool quantification, mitochondrial ADP-ribosylation, blue-native gel approach, 3D ... Læs mere

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  • Protein Aggregation

    Methods and Protocols

    Redigeret af Andrzej Stanisław Cieplak ...
    Serier serie Springer Protocols
    The volume details techniques, methods, and conceptual developments to further the study of protein aggregation with emphasis on the pleiomorphic proteins implicated in etiology of neurodegeneration. Chapters guide readers through in vitro and in vivo studies of fibrillization and liquid-liquid phase separation processes, and offer a comprehensive account of the state-of-art of structural studies ... Læs mere

    1.428,99 kr.

  • Oxygen Sensing

    Methods and Protocols

    Redigeret af Emily E. Weinert ...
    Serier serie Springer Protocols
    This volume provides researchers with protocols that help them investigate known or putative O2 sensing proteins and pathways. The chapters in this book discuss techniques ranging from anaerobic redox midpoint measurement to approaches to control expression of globin genes, which provide detailed methods for researchers interested in expanding their knowledge of O2 sensing systems. Written in the ... Læs mere

    1.307,45 kr.

  • Metabolic Reprogramming

    Methods and Protocols

    Redigeret af Salvatore Papa, Concetta Bubici ...
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    This volume provides state-of-the-art methods and protocols from researchers in the field of metabolism. Chapters guide readers through methodologies measuring different aspects of lipid metabolism, quantifying the amino acids, mitochondrial and intracellular by-products, detect enzyme and kinase activities, detecting a variety of metabolic pathways, and intracellular networks including nucleotide ... Læs mere

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  • Molecular Motors

    Methods and Protocols

    Serier serie Springer Protocols
    This fully updated volume presents a diverse collection of protocols and methodologies for studying various molecular motors. The book explores the dynamics of the bacterial flagellar motor, the mechanisms of ATP synthase, the assembly and function of cytoskeletal motors, and the role of nucleic acid motors in cellular processes, while placing a strong emphasis on state-of-the-art techniques, ... Læs mere

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  • Dictyostelium discoideum

    Methods and Protocols

    Redigeret af Alan R. Kimmel ...
    Serier serie Springer Protocols
    This volume discusses the latest advancements and techniques used to study Dictyostelium discoideum. The chapters in this book cover topics such as cytokinesis, membrane function and damage, and nutrient/bacterial processing; autophagy activation and function; protein localization, interaction, and activation; cell migration and contact guidance for quantification of cytoskeletal dynamics; and ... Læs mere

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  • Recombinant Protein Expression in Mammalian Cells

    Methods and Protocols

    Redigeret af David L. Hacker ...
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    This fully updated volume explores notable developments in the field of mammalian cell-based recombinant protein production. Beginning with methods for transient recombinant protein production, the book continues with methods for stable cell pool generation, protein production using stable clonal cell lines, as well as high-throughput screening technologies for characterizing transient cell ... Læs mere

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