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  • Cell Cycle Control

    Methods and Protocols

    Redigeret af Anna Castro, Benjamin Lacroix ...
    Serier serie Springer Protocols
    This detailed volume collects techniques to study the highly regulated cell cycle process. Beginning with chapters investigating these processes and assessing how cells respond when these complicated pathways are simplified by using synthetic biology and in vitro reconstitutions, the book continues by exploring how cells sense and respond to environmental conditions, different model systems and ... Læs mere

    1.396,46 kr.

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  • Drosophila

    Methods and Protocols

    Redigeret af Christian Dahmann ...
    Serier serie Springer Protocols
    This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and ... Læs mere

    1.021,24 kr.

  • Zebrafish

    Methods and Protocols

    Serier serie Springer Protocols
    This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the ... Læs mere

    1.641,99 kr.

  • DNA-Protein Interactions

    Methods and Protocols

    Redigeret af Marcos Simoes-Costa ...
    Serier serie Springer Protocols
    This volume details protocols emphasizing systems-level approaches that can be applied to genomic analyses. Chapters detail techniques for optimized application in in vivo systems, spatial, physiological, environmental contexts, imaging-based techniques, single-molecule approaches, CRISPR systems, new genomic approaches, and measurements of kinetics governing. Written in the format of the highly ... Læs mere

    1.021,24 kr.

  • Histones

    Methods and Protocols

    Redigeret af Luc Gaudreau, Benoit Guillemette ...
    Serier serie Springer Protocols
    This second edition provides new and updated chapters on histones for molecular biologists, biochemists and geneticists. Chapters cover protein complexes that modify chromatin either by adding or removing post-translational modifications, or by exchanging histone variants within the nucleosome. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief ... Læs mere

    2.138,77 kr.

  • Dictyostelium discoideum

    Methods and Protocols

    Redigeret af Alan R. Kimmel ...
    Serier serie Springer Protocols
    This volume discusses the latest advancements and techniques used to study Dictyostelium discoideum. The chapters in this book cover topics such as cytokinesis, membrane function and damage, and nutrient/bacterial processing; autophagy activation and function; protein localization, interaction, and activation; cell migration and contact guidance for quantification of cytoskeletal dynamics; and ... Læs mere

    1.335,49 kr.

  • Mitotic Exit

    Methods and Protocols

    Redigeret af Victor M. Bolanos-Garcia ...
    Serier serie Springer Protocols
    This volume explores the latest approaches and techniques used to study mitotic exit in diverse model organisms. The chapters in this book cover topics such as transgenic methods generation of RNAi-sensitive cell lines; gene overexpression in heterologue gene expression systems; quantitative live cell imaging and FRET-FLIM; biochemical protocols for analyzing post-translational modifications ... Læs mere

    1.840,86 kr.

  • Spatial Genome Organization

    Methods and Protocols

    Redigeret af Tom Sexton ...
    Serier serie Springer Protocols
    This detailed volume explores a variety of cutting-edge techniques used to interrogate spatial genome organization. Beginning with a section covering the vital chromosome conformation capture (3C) technique, this collection continues with chapters on targeted Hi-C approaches, sequencing-based approaches to assess nuclear environment, as well as single-cell technologies to better characterize the ... Læs mere

    1.400,85 kr.

  • Mitochondrial DNA

    Methods and Protocols

    Serier serie Springer Protocols
    This volume compiles a comprehensive range of methods to study key aspects of mitochondrial DNA including nucleoid structure and packaging, replication, genome integrity, and disease. Chapters are organized into eight methodological sections that cover in vitro and in vivo methods, including for mtDNA isolation, visualization, deep sequencing, gene editing, and diagnostic aspects of mtDNA disease. ... Læs mere

    1.756,86 kr.

  • piRNA

    Methods and Protocols

    Serier serie Springer Protocols
    This detailed volume explores newly-developed methods in PIWI-interacting RNAs (piRNAs) research, methods currently applied to other ncRNAs involved in nuclear regulation which can be used to study piRNAs, and piRNA methods applied in non-classical organisms. It also includes several bioinformatic and biophysical methods related to piRNA studies, consistent with the increasing importance of high ... Læs mere

    1.260,86 kr.

  • Pichia pastoris

    Methods and Protocols

    Serier serie Springer Protocols
    This volume provides readers with a useful and comprehensive resource of laboratory protocols for working with Pichia pastoris. The chapters in this book are organized into seven parts: introduction, bioinformatics, cell and systems biology, gene expression cassettes and vectors, Pichia pastoris expression strains, upstream bioprocessing, and downstream processing and product analysis. This book ... Læs mere

    1.414,11 kr.

  • Prokaryotic Gene Regulation

    Methods and Protocols

    Redigeret af Eveline Peeters, Indra Bervoets ...
    Serier serie Springer Protocols
    This volume presents a collection of versatile methodologies to investigate prokaryotic gene regulation, with focus on the different levels of information processing and usefulness for various model organisms, whether archaeal, bacterial, or both. The chapters in this book are divided into four sections. Section One covers methods that enable the study of the structure of the bacterial/archaeal ... Læs mere

    1.400,85 kr.