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  • Therapeutic Proteins

    Methods and Protocols

    Serier serie Springer Protocols
    This volume covers the latest key aspects of therapeutic protein applications. Chapters in this book cover topics such as the discovery, production, and conjugation of protein-proteins with discussions on the direction of future development and advancements; ways to use these engineering proteins for therapeutic and vaccine applications; and the use of modified protein nanocarriers. Written in the ... Læs mere

    1.092,74 kr.

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  • Antibody Engineering Volume 1

    Serier serie Springer Protocols
    Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new ... Læs mere

    3.166,13 kr.

  • Single-Domain Antibodies

    Methods and Protocols

    Redigeret af Greg Hussack, Kevin A. Henry ...
    Serier serie Springer Protocols
    This volume covers current and emerging techniques for studying single-domain antibodies (sdAbs). Chapters guide readers through the biology and immunology of sdAbs in camelids and sharks, isolation of sdAbs, protein engineering approaches to optimize the solubility, stability, valency and antigen binding affinity of sdAbs, and specialized applications of sdAbs. Written in the format of the highly ... Læs mere

    1.681,11 kr.

  • Lipopolysaccharide Transport

    Methods and Protocols

    Redigeret af Paola Sperandeo ...
    Serier serie Springer Protocols
    This volume provides the most widely used protocols and techniques employed in lipopolysaccharide( LPS) transport studies. Chapters detail identification and analysis of essential genes and crucial mutations in LPS transport-related genes, LPS profile determination, LPS chemical analysis, protein-LPS interaction, and LPS visualization. Written in the format of the highly successful Methods in ... Læs mere

    933,87 kr.

  • Metabolic Reprogramming

    Methods and Protocols

    Redigeret af Salvatore Papa, Concetta Bubici ...
    Serier serie Springer Protocols
    This volume provides state-of-the-art methods and protocols from researchers in the field of metabolism. Chapters guide readers through methodologies measuring different aspects of lipid metabolism, quantifying the amino acids, mitochondrial and intracellular by-products, detect enzyme and kinase activities, detecting a variety of metabolic pathways, and intracellular networks including nucleotide ... Læs mere

    1.176,74 kr.

  • Recombinant Glycoproteins

    Methods and Protocols

    Redigeret af Steven B. Bradfute ...
    Serier serie Springer Protocols
    This detailed volume explores the production and purification of glycoproteins for therapeutic use or basic research, as well as the necessary in-depth understanding of cellular and acellular systems available for these purposes, their advantages and disadvantages, and considerations for choosing the most appropriate system for the desired application. Beginning with chapters on viral-encoded ... Læs mere

    1.099,74 kr.

  • Mitochondrial Medicine

    Volume 2: Assessing Mitochondria

    Redigeret af Volkmar Weissig, Marvin Edeas ...
    Serier serie Springer Protocols
    This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume II guides readers through chapters on mitochondrial dysfunction, functional’ mitochondria, mitochondrial retrograde, mitochondrial dNTP pool quantification, mitochondrial ADP-ribosylation, blue-native gel approach, 3D ... Læs mere

    942,61 kr.

  • Mitotic Exit

    Methods and Protocols

    Redigeret af Victor M. Bolanos-Garcia ...
    Serier serie Springer Protocols
    This volume explores the latest approaches and techniques used to study mitotic exit in diverse model organisms. The chapters in this book cover topics such as transgenic methods generation of RNAi-sensitive cell lines; gene overexpression in heterologue gene expression systems; quantitative live cell imaging and FRET-FLIM; biochemical protocols for analyzing post-translational modifications ... Læs mere

    1.840,86 kr.

  • Base Excision Repair Pathway

    Methods and Protocols

    Redigeret af Kishor K. Bhakat, Tapas K. Hazra ...
    Serier serie Springer Protocols
    This detailed volume provides a comprehensive set of experimental protocols and useful strategies to examine the repair of damaged bases via the Base Excision Repair (BER) pathway in vitro and in cells. Beginning with multiple molecular and cellular techniques to examine the excision of damaged bases from double-stranded DNA or DNA wrapped in a nucleosome, the book continues with sections covering ... Læs mere

    1.309,23 kr.

  • Heterologous Expression of Membrane Proteins

    Methods and Protocols

    Redigeret af Isabelle Mus-Veteau ...
    Serier serie Springer Protocols
    This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus ... Læs mere

    1.400,85 kr.

  • Iron Metabolism

    Methods and Protocols

    Redigeret af Oleh Khalimonchuk ...
    Serier serie Springer Protocols
    This detailed volume explores classical and cutting-edge methods optimized and validated to analyze various aspects of iron metabolism, from in vitro to multi-organ level. Opening with a section on basic iron metabolism methods, the book continues with methods applicable to a variety of systems, ranging from bacteria to cultured mammalian cells and tissues, with a focus on cellular heme and iron ... Læs mere

    1.344,86 kr.

  • DNA Damage Detection

    Methods and Protocols

    Redigeret af Takamitsu A. Kato ...
    Serier serie Springer Protocols
    This volume explores techniques in DNA damage analysis, important not only for understanding cellular health after exposure to genotoxic agents but also for assessing cellular ability of genome maintenance. The book explores traditional DNA damage measurement methods, as well as modern fluorescence-based analysis and high throughput automatic analysis methods. Written for the highly successful ... Læs mere

    1.681,11 kr.