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  • Bioconjugation Methods

    Redigeret af Alexander Deiters ...
    Serier serie Springer Protocols
    This volume presents cutting-edge chemical and molecular strategies. Chapters detail bioorthogonal cycloadditions, electrophiles, self‑labeling systems, noncanonical amino acids, site‑selective bioconjugation across proteins, nucleic acids, cells, and viral assemblies.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective ... Læs mere

    1.868,14 kr.

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  • Antibody Engineering Volume 1

    Serier serie Springer Protocols
    Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new ... Læs mere

    3.166,66 kr.

  • Therapeutic Proteins

    Methods and Protocols

    Serier serie Springer Protocols
    This volume covers the latest key aspects of therapeutic protein applications. Chapters in this book cover topics such as the discovery, production, and conjugation of protein-proteins with discussions on the direction of future development and advancements; ways to use these engineering proteins for therapeutic and vaccine applications; and the use of modified protein nanocarriers. Written in the ... Læs mere

    1.214,26 kr.

  • Proteases and Cancer

    Methods and Protocols

    Redigeret af Salvatore Santamaria ...
    Serier serie Springer Protocols
    This volume details bioinformatics analysis, biochemical assays, recombinant protein expression and purification, methods to investigate protease activity in cell-based, organoids and in vivo systems, proteomics, transcriptomics, machine learning and novel approaches to target dysregulated protease activity in cancer. Written in the highly successful Methods in Molecular Biology series format, ... Læs mere

    1.222,11 kr.

  • Bacterial Secretion Systems

    Methods and Protocols

    Redigeret af Laure Journet, Eric Cascales ...
    Serier serie Springer Protocols
    This second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or ... Læs mere

    1.999,20 kr.

  • Macrophage Migration Inhibitory Factor

    Methods and Protocols

    Redigeret af James Harris, Timothy W. Muusse ...
    Serier serie Springer Protocols
    This second edition volume expands on the previous edition with new protocols that offer biochemical, biophysical, and biological assays for studying macrophage migration inhibitory factor (MIF) and D-dopachrome tautomerase (D-DT). The chapters in this book cover topics such as generation of MIF peptides via solid phase peptide synthesis; fragment screening of MIF by surface plasmon resonance; MIF ... Læs mere

    1.868,14 kr.

  • Lipopolysaccharide Transport

    Methods and Protocols

    Redigeret af Paola Sperandeo ...
    Serier serie Springer Protocols
    This volume provides the most widely used protocols and techniques employed in lipopolysaccharide( LPS) transport studies. Chapters detail identification and analysis of essential genes and crucial mutations in LPS transport-related genes, LPS profile determination, LPS chemical analysis, protein-LPS interaction, and LPS visualization. Written in the format of the highly successful Methods in ... Læs mere

    934,02 kr.

  • Protein Tyrosine Phosphatases

    Methods and Protocols

    Serier serie Springer Protocols
    This second edition volume expands on the previous edition with discussions on the latest advancements in protein tyrosine phosphatases (PTP) research used to investigate these essential enzymes and new inhibitors. The new techniques covered in the chapters of this book include studying enzymes in vitro, in cells, and in animal models through proteomics, genomics, and structural biology. ... Læs mere

    1.484,03 kr.

  • Mitochondrial Medicine

    Volume 2: Assessing Mitochondria

    Redigeret af Volkmar Weissig, Marvin Edeas ...
    Serier serie Springer Protocols
    This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume II guides readers through chapters on mitochondrial dysfunction, functional’ mitochondria, mitochondrial retrograde, mitochondrial dNTP pool quantification, mitochondrial ADP-ribosylation, blue-native gel approach, 3D ... Læs mere

    1.047,52 kr.

  • Recombinant Glycoproteins

    Methods and Protocols

    Redigeret af Steven B. Bradfute ...
    Serier serie Springer Protocols
    This detailed volume explores the production and purification of glycoproteins for therapeutic use or basic research, as well as the necessary in-depth understanding of cellular and acellular systems available for these purposes, their advantages and disadvantages, and considerations for choosing the most appropriate system for the desired application. Beginning with chapters on viral-encoded ... Læs mere

    1.222,11 kr.

  • Antigen Processing and Presentation

    Methods and Protocols

    Redigeret af Efstratios Stratikos ...
    Serier serie Springer Protocols
    This volume discusses the latest advancements in the field of antigen processing and presentation, and the complex assays and techniques that have been devised to help pick apart molecular events along this pathway. The chapters in this book cover topics such as how to make and analyze MHC molecules and their cargo; analyzing the function chaperones that regulate antigen presentation; enzymes that ... Læs mere

    1.681,32 kr.

  • Bacterial Regulatory RNA

    Methods and Protocols

    Serier serie Springer Protocols
    This second edition details new and updated methods used for studying prokaryotic non-coding RNAs and their protein accomplices. Chapters detail discovery of ncRNAs, characterization of their structure, functions, and their interactomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary ... Læs mere

    1.222,11 kr.