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Single Cell ‘Omics of Neuronal Cells

Redigeret af Jonathan V. Sweedler, James Eberwine, Scott E. Fraser ...

Serier serie Springer Protocols
This volume discusses the latest techniques used in the diverse fields of single cell ‘omics and covers topics such as quantifying the single cell transcriptome; isolation of cells in nanoliter volumes for single cell proteomics measurements by nano-LC-MS/MS; and single cell protein characterization by immunoblotting. A wide range of methodologies are highlighted, ranging from high-yield chemical ... Læs mere

1.400,85 kr.

This volume discusses the latest techniques used in the diverse fields of single cell ‘omics and covers topics such as quantifying the single cell transcriptome; isolation of cells in nanoliter volumes for single cell proteomics measurements by nano-LC-MS/MS; and single cell protein characterization by immunoblotting. A wide range of methodologies are highlighted, ranging from high-yield chemical
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Chemical Genomics

Redigeret af Haian Fu, PhD ...

Advances in chemistry, biology and genomics coupled with laboratory automation and computational technologies have led to the rapid emergence of the multidisciplinary field of chemical genomics. This edited text, with contributions from experts in the field, discusses the new techniques and applications that help further the study of chemical genomics. The beginning chapters provide an overview of ... Læs mere

1.092,59 kr.

Advances in chemistry, biology and genomics coupled with laboratory automation and computational technologies have led to the rapid emergence of the multidisciplinary field of chemical genomics. This edited text, with contributions from experts in the field, discusses the new techniques and applications that help further the study of chemical genomics. The beginning chapters provide an overview of
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Drosophila

Methods and Protocols

Redigeret af Christian Dahmann ...

Serier serie Springer Protocols
This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and ... Læs mere

1.021,24 kr.

This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and
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Molecular Analysis and Genome Discovery

Redigeret af Ralph Rapley, Stuart Harbron ...

Molecular Analysis and Genome Discovery, Second Edition is a completely revised and updated new edition of this successful book. The text provides a comprehensive overview of recent developments in the fast moving field of molecular based diagnostics of disease markers. Key concepts and applications are provided alongside practical information on current techniques currently being researched and ... Læs mere

876,99 kr.

Molecular Analysis and Genome Discovery, Second Edition is a completely revised and updated new edition of this successful book. The text provides a comprehensive overview of recent developments in the fast moving field of molecular based diagnostics of disease markers. Key concepts and applications are provided alongside practical information on current techniques currently being researched and
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Protein and Peptide Mass Spectrometry in Drug Discovery

Redigeret af Michael L. Gross, Guodong Chen, Birendra Pramanik ...

The book that highlights mass spectrometry and its application in characterizing proteins and peptides in drug discoveryAn instrumental analytical method for quantifying the mass and characterization of various samples from small molecules to large proteins, mass spectrometry (MS) has become one of the most widely used techniques for studying proteins and peptides over the last decade. Bringing ... Læs mere

1.202,36 kr.

The book that highlights mass spectrometry and its application in characterizing proteins and peptides in drug discoveryAn instrumental analytical method for quantifying the mass and characterization of various samples from small molecules to large proteins, mass spectrometry (MS) has become one of the most widely used techniques for studying proteins and peptides over the last decade. Bringing
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Proteomics Sample Preparation

Redigeret af Jörg von Hagen ...

This long-awaited first guide to sample preparation for proteomics studies overcomes a major bottleneck in this fast growing technique within the molecular life sciences. By addressing the topic from three different angles -- sample, method and aim of the study -- this practical reference has something for every proteomics researcher. Following an introduction to the field, the book looks at ... Læs mere

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This long-awaited first guide to sample preparation for proteomics studies overcomes a major bottleneck in this fast growing technique within the molecular life sciences. By addressing the topic from three different angles -- sample, method and aim of the study -- this practical reference has something for every proteomics researcher. Following an introduction to the field, the book looks at
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Analytical Characterization of Biotherapeutics

Redigeret af Jennie R. Lill, Wendy Sandoval ...

The definitive guide to the myriad analytical techniques available to scientists involved in biotherapeutics researchAnalytical Characterization of Biotherapeutics covers all current and emerging analytical tools and techniques used for the characterization of therapeutic proteins and antigen reagents. From basic recombinant antigen and antibody characterization, to complex analyses for ... Læs mere

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The definitive guide to the myriad analytical techniques available to scientists involved in biotherapeutics researchAnalytical Characterization of Biotherapeutics covers all current and emerging analytical tools and techniques used for the characterization of therapeutic proteins and antigen reagents. From basic recombinant antigen and antibody characterization, to complex analyses for
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Methods and Protocols

Redigeret af Yanzhuang Wang, Vladimir V Lupashin, Todd R. Graham ...

Serier serie Springer Protocols
This volume provides readers with a collection of new and classical methods, techniques, and applications used to address enduring questions about the structure and functions of the Golgi complex. The chapters in this volume cover diverse topics ranging from model systems; live and fixed cell imaging techniques; in vitro biochemical reconstitution systems; and specific methods developed to study ... Læs mere

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This volume provides readers with a collection of new and classical methods, techniques, and applications used to address enduring questions about the structure and functions of the Golgi complex. The chapters in this volume cover diverse topics ranging from model systems; live and fixed cell imaging techniques; in vitro biochemical reconstitution systems; and specific methods developed to study
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Redigeret af Peter Scheiffele, Oriane Mauger ...

Serier serie Springer Protocols
This detailed volume collects commonly used and cutting-edge methods to analyze alternative splicing, a key step in gene regulation. After an introduction of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in complex biological systems, visualizing and localizing alternative spliced ... Læs mere

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This detailed volume collects commonly used and cutting-edge methods to analyze alternative splicing, a key step in gene regulation. After an introduction of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in complex biological systems, visualizing and localizing alternative spliced
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Methods and Protocols

Redigeret af Wayne F. Anderson ...

Serier serie Springer Protocols
Structural Genomics and Drug Discovery: Methods and Protocols focuses on high throughput structure determination methods and how they can be applied to lay the groundwork for structure aided drug discovery. The methods and protocols that are described can be applied in any laboratory interested in using detailed structural information to advance the initial stages of drug discovery. Written in the ... Læs mere

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Redigeret af Tsuyoshi Hirota, Megumi Hatori, Satchidananda Panda ...

Serier serie Springer Protocols
This volume provides backgrounds, methods, and troubleshooting for the analysis of circadian rhythms at the molecular, cellular, and organismal levels in vertebrates and invertebrates, and also covers light input, sleep, circalunar rhythms, and seasonal rhythms. The chapters in this book discuss behavioral and biological testing, histochemistry, single-cell imaging, electrophysiology, ... Læs mere

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This volume provides backgrounds, methods, and troubleshooting for the analysis of circadian rhythms at the molecular, cellular, and organismal levels in vertebrates and invertebrates, and also covers light input, sleep, circalunar rhythms, and seasonal rhythms. The chapters in this book discuss behavioral and biological testing, histochemistry, single-cell imaging, electrophysiology,
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Methods and Protocols

Redigeret af Tamas Dalmay ...

Serier serie Springer Protocols
This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe ... Læs mere

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This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe
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Single Cell ‘Omics of Neuronal Cells

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Molecular Analysis and Genome Discovery

Protein and Peptide Mass Spectrometry in Drug Discovery

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Circadian Clocks

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