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  • Genotyping

    Methods and Protocols

    Redigeret af Anthony Torres ...
    Serier serie Springer Protocols
    This volume covers the latest techniques used to study single nucleotide polymorphisms (SNP) genotyping, such as Sanger sequencing, qPCR, Nanopore sequencing, and PACE. The chapters in this book cover topics such as SNP nomenclature, databases, and significance of SNPs in various fields; PCR-based techniques like HRM and CD-PCR; isothermal amplification methodologies; high-throughput methods like ... Læs mere

    1.681,11 kr.

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  • Drosophila

    Methods and Protocols

    Redigeret af Christian Dahmann ...
    Serier serie Springer Protocols
    This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and ... Læs mere

    1.021,24 kr.

  • Catharanthus roseus

    Methods and Protocols

    Serier serie Springer Protocols
    This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in C. roseus from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, transcription factor characterization up to functional genomic tools based on gene overexpression. Written in the format of the highly successful Methods in ... Læs mere

    1.867,83 kr.

  • Mass Spectrometry-Based Proteomics

    Redigeret af Kris Gevaert ...
    Serier serie Springer Protocols
    This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by ... Læs mere

    1.414,11 kr.

  • Clinical Applications of Nucleic Acid Amplification

    Serier serie Springer Protocols
    This volume details applications, methods, and protocols on nucleic acid amplification. Chapters detail the management of organ and stem cell transplantation, constitutional genetics and genomics, epidemiology, public health, nucleic acid detection, methods for amplification and sequencing, nuclease-assisted mutant allele enrichment, isothermal, and high-throughput sequencing. Written in the ... Læs mere

    1.176,74 kr.

  • DNA-Protein Interactions

    Methods and Protocols

    Redigeret af Marcos Simoes-Costa ...
    Serier serie Springer Protocols
    This volume details protocols emphasizing systems-level approaches that can be applied to genomic analyses. Chapters detail techniques for optimized application in in vivo systems, spatial, physiological, environmental contexts, imaging-based techniques, single-molecule approaches, CRISPR systems, new genomic approaches, and measurements of kinetics governing. Written in the format of the highly ... Læs mere

    1.021,24 kr.

  • Schizosaccharomyces pombe

    Methods and Protocols

    Redigeret af Ruben C. Petreaca ...
    Serier serie Springer Protocols
    This detailed volume explores techniques utilizing the fission yeast Schizosaccharomyces pombe, a powerful tractable model system for eukaryotic biology. The book begins with a section on microscopy and imaging, and it continues with chapters covering genome and plasmid editing, including the use of CRISPR/Cas9 technologies, as well as the analysis of DNA damage, repair, and recombination, and ... Læs mere

    1.512,99 kr.

  • Euglenozoa

    Methods and Protocols, Volume 1

    Serier serie Springer Protocols
    This detailed collection covers methods recently developed for experiments on organisms from each of the major euglenozoan groups, including diplonemids, euglenids, and kinetoplastids. Volume 1 presents a historical review of the research into euglenozoans, followed by sections of techniques involving isolation and culturing of different species of euglenozoans, as well as a variety of -omics ... Læs mere

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  • Digital PCR

    Methods and Protocols

    Redigeret af Guillaume Pavlovic ...
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    This detailed volume explores digital polymerase chain reaction (dPCR), a third generation PCR technology that enables absolute quantification by counting positive and negative partitions after endpoint amplification. The book aims to provide an overview of the various possibilities currently available, both in terms of instrumentation and applications. The selected protocols cover a wide range of ... Læs mere

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  • Meiosis

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    Redigeret af Jesús A Carballo ...
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    This volume details protocols on meiosis, covering the latest technological and methodological advances in this research field. Chapters guide readers through methods on genomics, biochemistry, super-resolution microscopy, traditional genetics, cytological methods, as well as machine learning and in silico modelling. Written in the highly successful Methods in Molecular Biology series format, ... Læs mere

    1.840,86 kr.

  • Recombinant Protein Expression in Mammalian Cells

    Methods and Protocols

    Redigeret af David L. Hacker ...
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    This fully updated volume explores notable developments in the field of mammalian cell-based recombinant protein production. Beginning with methods for transient recombinant protein production, the book continues with methods for stable cell pool generation, protein production using stable clonal cell lines, as well as high-throughput screening technologies for characterizing transient cell ... Læs mere

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  • Plant Cytogenetics and Cytogenomics

    Methods and Protocols

    Redigeret af Tony Heitkam, Sònia Garcia ...
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    This book provides an up-to-date account of the most widespread methods used by specialists in the field of plant cytogenetics and the emerging field of cytogenomics that will likely soon be adapted by more labs. From the classical basic karyological approaches to the most recent genomics-informed and computational methods, the volume explores genome size and ploidy level estimation, chromosome ... Læs mere

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