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  • Digital PCR

    Methods and Protocols

    Redigeret af Guillaume Pavlovic ...
    Serier serie Springer Protocols
    This detailed volume explores digital polymerase chain reaction (dPCR), a third generation PCR technology that enables absolute quantification by counting positive and negative partitions after endpoint amplification. The book aims to provide an overview of the various possibilities currently available, both in terms of instrumentation and applications. The selected protocols cover a wide range of ... Læs mere

    1.681,04 kr.

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  • Drosophila

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    Redigeret af Christian Dahmann ...
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    This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and ... Læs mere

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  • Mass Spectrometry-Based Proteomics

    Redigeret af Kris Gevaert ...
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    This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by ... Læs mere

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  • Methods for Plant Nucleus and Chromatin Studies

    Methods and Protocols

    Redigeret af Célia Baroux, Christophe Tatout ...
    Serier serie Springer Protocols
    This volume provides a comprehensive collection of protocols that can be used to study plant chromatin structure and composition. Chapters divided into five sections detail the profiling of chromatin features in relation to epigenetic regulation, investigate the interaction between chromatin modifications and gene regulation, and explore the 3D spatial organization of the chromatin inside the ... Læs mere

    1.924,99 kr.

  • MicroRNA Detection and Target Identification

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    Redigeret af Tamas Dalmay ...
    Serier serie Springer Protocols
    This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe ... Læs mere

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  • DNA Nanotechnology

    Methods and Protocols

    Redigeret af Giampaolo Zuccheri ...
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    This fully updated volume explores methods to create and use nanostructures for characterizing, targeting, and controlling the behavior of cells. Experts from around the globe present their procedures for designing, preparing, characterizing, and using DNA nanostructures, especially towards biological or materials science applications. Additionally, the study of fundamental aspects of nucleic ... Læs mere

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  • Optogenetics

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    Redigeret af Armin Baumschlager ...
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    This volume explores the latest advancements in the field of optogenetics and how it uses cellular light-sensing components and genetic engineering to control proteins and biological processes. The book chapters are organized into four parts. Part One focuses on intracellular optogenetic components for control of specific cell functions; Part Two looks at externally supplied light regulators that ... Læs mere

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  • SNAREs

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    Redigeret af Rutilio Fratti ...
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    This second edition details new and updated methods on soluble N-ethylmaleimide-sensitive factor attachment protein receptors( SNAREs) and their function are examined in the laboratory. Chapters guide readers through an overview of the basic properties of SNAREs, distribution and interaction with regulators of membrane fusion, activation of SNAREs in the priming stage by NSF/Sec18 and a-SNAP/Sec17 ... Læs mere

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  • piRNA

    Methods and Protocols

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    This detailed volume explores newly-developed methods in PIWI-interacting RNAs (piRNAs) research, methods currently applied to other ncRNAs involved in nuclear regulation which can be used to study piRNAs, and piRNA methods applied in non-classical organisms. It also includes several bioinformatic and biophysical methods related to piRNA studies, consistent with the increasing importance of high ... Læs mere

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  • Recombinant Protein Expression in Mammalian Cells

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    Redigeret af David L. Hacker ...
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    This fully updated volume explores notable developments in the field of mammalian cell-based recombinant protein production. Beginning with methods for transient recombinant protein production, the book continues with methods for stable cell pool generation, protein production using stable clonal cell lines, as well as high-throughput screening technologies for characterizing transient cell ... Læs mere

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  • Genotyping

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    Redigeret af Anthony Torres ...
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    This volume covers the latest techniques used to study single nucleotide polymorphisms (SNP) genotyping, such as Sanger sequencing, qPCR, Nanopore sequencing, and PACE. The chapters in this book cover topics such as SNP nomenclature, databases, and significance of SNPs in various fields; PCR-based techniques like HRM and CD-PCR; isothermal amplification methodologies; high-throughput methods like ... Læs mere

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  • Histones

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    Redigeret af Luc Gaudreau, Benoit Guillemette ...
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    This second edition provides new and updated chapters on histones for molecular biologists, biochemists and geneticists. Chapters cover protein complexes that modify chromatin either by adding or removing post-translational modifications, or by exchanging histone variants within the nucleosome. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief ... Læs mere

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