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  • Methods for Plant Nucleus and Chromatin Studies

    Methods and Protocols

    Redigeret af Célia Baroux, Christophe Tatout ...
    Serier serie Springer Protocols
    This volume provides a comprehensive collection of protocols that can be used to study plant chromatin structure and composition. Chapters divided into five sections detail the profiling of chromatin features in relation to epigenetic regulation, investigate the interaction between chromatin modifications and gene regulation, and explore the 3D spatial organization of the chromatin inside the ... Læs mere

    1.924,99 kr.

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  • Drosophila

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    Redigeret af Christian Dahmann ...
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    This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and ... Læs mere

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  • Catharanthus roseus

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    This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in C. roseus from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, transcription factor characterization up to functional genomic tools based on gene overexpression. Written in the format of the highly successful Methods in ... Læs mere

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  • Mass Spectrometry-Based Proteomics

    Redigeret af Kris Gevaert ...
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    This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by ... Læs mere

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  • piRNA

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    This detailed volume explores newly-developed methods in PIWI-interacting RNAs (piRNAs) research, methods currently applied to other ncRNAs involved in nuclear regulation which can be used to study piRNAs, and piRNA methods applied in non-classical organisms. It also includes several bioinformatic and biophysical methods related to piRNA studies, consistent with the increasing importance of high ... Læs mere

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  • mRNA Decay

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    Redigeret af Maxime Wery ...
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    This detailed volume explores techniques for researching the diverse and specialized mechanisms for mRNA degradation, both in the cytoplasm and the nucleus. From classical methods for studying RNA degradation at the single RNA level to the latest transcriptome-wide approaches involving long-read sequencing and metabolic labeling, this book focuses on methods for eukaryotic models, such as ... Læs mere

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  • Histones

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    Redigeret af Luc Gaudreau, Benoit Guillemette ...
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    This second edition provides new and updated chapters on histones for molecular biologists, biochemists and geneticists. Chapters cover protein complexes that modify chromatin either by adding or removing post-translational modifications, or by exchanging histone variants within the nucleosome. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief ... Læs mere

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  • SNAREs

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    Redigeret af Rutilio Fratti ...
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    This second edition details new and updated methods on soluble N-ethylmaleimide-sensitive factor attachment protein receptors( SNAREs) and their function are examined in the laboratory. Chapters guide readers through an overview of the basic properties of SNAREs, distribution and interaction with regulators of membrane fusion, activation of SNAREs in the priming stage by NSF/Sec18 and a-SNAP/Sec17 ... Læs mere

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  • Peptide Libraries

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    This volume provides a collection of novel and emerging methods for the generation and application of peptide libraries. Chapter focus on methods and techniques highlighting new avenues for library screening. Written in the Methods in Molecular Biology series format, chapters outline strategies and overview their area or describe specific applications of the method including an introduction, the ... Læs mere

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  • R-Loops

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    Redigeret af Andrés Aguilera, Alexey Ruzov ...
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    This detailed book compiles a series of laboratory protocols covering the most important aspects of R-loop biology. Beginning with a range of methods allowing for the detection of DNA-RNA hybrids, as well as their purification and visualization by electron microscopy, the volume continues with methods based on the use of RNase H-derived tools to detect DNA-RNA hybrids in vitro and in vivo. Several ... Læs mere

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  • MicroRNA Detection and Target Identification

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    This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe ... Læs mere

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  • Digital PCR

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    This detailed volume explores digital polymerase chain reaction (dPCR), a third generation PCR technology that enables absolute quantification by counting positive and negative partitions after endpoint amplification. The book aims to provide an overview of the various possibilities currently available, both in terms of instrumentation and applications. The selected protocols cover a wide range of ... Læs mere

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