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Translational Research Methods for Tauopathies

Redigeret af Jessica S. Fortin ...

Serier serie Springer Protocols
This volume provides the novice to expert tau scientist with detailed protocols to establish in vitro and in vivo techniques designed for understanding the physiopathology of tau in neurodegenerative diseases. The protocols in this book were intentionally selected to provide a basis for research laboratories focusing on tau neurodegenerative drug discovery, and are organized into four cohesive ... Læs mere

1.868,14 kr.

This volume provides the novice to expert tau scientist with detailed protocols to establish in vitro and in vivo techniques designed for understanding the physiopathology of tau in neurodegenerative diseases. The protocols in this book were intentionally selected to provide a basis for research laboratories focusing on tau neurodegenerative drug discovery, and are organized into four cohesive
... Læs mere

Zebrafish

Methods and Protocols

Redigeret af James F. Amatruda, Corinne Houart, Koichi Kawakami, Kenneth D. Poss ...

Serier serie Springer Protocols
This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the ... Læs mere

1.824,61 kr.

This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the
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In Vitro Bioassay Techniques for Anticancer Drug Discovery and Development

af Dhanya Sunil, Raghu Chandrashekhar H, Pooja Kamath ...

This comprehensive and useful handbook represents a definitive up-to-date compendium of key in vitro bioassay methods that are employed to quantify and validate the anticancer activity of a drug candidate before it makes its way in to animal or clinical trials.In Vitro Bioassay Techniques for Anticancer Drug Discovery and Development covers the screening and evaluation of potential drug candidates ... Læs mere

462,69 kr.

This comprehensive and useful handbook represents a definitive up-to-date compendium of key in vitro bioassay methods that are employed to quantify and validate the anticancer activity of a drug candidate before it makes its way in to animal or clinical trials.In Vitro Bioassay Techniques for Anticancer Drug Discovery and Development covers the screening and evaluation of potential drug candidates
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Proteases and Cancer

Methods and Protocols

Redigeret af Salvatore Santamaria ...

Serier serie Springer Protocols
This volume details bioinformatics analysis, biochemical assays, recombinant protein expression and purification, methods to investigate protease activity in cell-based, organoids and in vivo systems, proteomics, transcriptomics, machine learning and novel approaches to target dysregulated protease activity in cancer. Written in the highly successful Methods in Molecular Biology series format, ... Læs mere

1.222,11 kr.

This volume details bioinformatics analysis, biochemical assays, recombinant protein expression and purification, methods to investigate protease activity in cell-based, organoids and in vivo systems, proteomics, transcriptomics, machine learning and novel approaches to target dysregulated protease activity in cancer. Written in the highly successful Methods in Molecular Biology series format,
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Macrophage Migration Inhibitory Factor

Methods and Protocols

Redigeret af James Harris, Timothy W. Muusse ...

Serier serie Springer Protocols
This second edition volume expands on the previous edition with new protocols that offer biochemical, biophysical, and biological assays for studying macrophage migration inhibitory factor (MIF) and D-dopachrome tautomerase (D-DT). The chapters in this book cover topics such as generation of MIF peptides via solid phase peptide synthesis; fragment screening of MIF by surface plasmon resonance; MIF ... Læs mere

1.868,14 kr.

This second edition volume expands on the previous edition with new protocols that offer biochemical, biophysical, and biological assays for studying macrophage migration inhibitory factor (MIF) and D-dopachrome tautomerase (D-DT). The chapters in this book cover topics such as generation of MIF peptides via solid phase peptide synthesis; fragment screening of MIF by surface plasmon resonance; MIF
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Immune Mediators in Cancer

Methods and Protocols

Redigeret af Ivana Vancurova, Yan Zhu ...

Serier serie Springer Protocols
This second edition provides a comprehensive collection of classic and cutting-edge methodologies as well as bioinformatics and genome-editing approaches for cancer cells and tissues. Chapters beginning with the Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step ... Læs mere

1.868,14 kr.

This second edition provides a comprehensive collection of classic and cutting-edge methodologies as well as bioinformatics and genome-editing approaches for cancer cells and tissues. Chapters beginning with the Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step
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Peptide Antibodies

Methods and Protocols

Redigeret af Gunnar Houen ...

Serier serie Springer Protocols
This detailed new edition explores current methods for the production and use of peptide antibodies. The book delves into various aspects of peptide synthesis and analysis, peptide-carrier conjugation, epitope and paratope prediction and identification, as well as improved assays and other uses of peptide antibodies. Written for the highly successful Methods in Molecular Biology series, chapters ... Læs mere

1.587,91 kr.

This detailed new edition explores current methods for the production and use of peptide antibodies. The book delves into various aspects of peptide synthesis and analysis, peptide-carrier conjugation, epitope and paratope prediction and identification, as well as improved assays and other uses of peptide antibodies. Written for the highly successful Methods in Molecular Biology series, chapters
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Antigen Processing and Presentation

Methods and Protocols

Redigeret af Efstratios Stratikos ...

Serier serie Springer Protocols
This volume discusses the latest advancements in the field of antigen processing and presentation, and the complex assays and techniques that have been devised to help pick apart molecular events along this pathway. The chapters in this book cover topics such as how to make and analyze MHC molecules and their cargo; analyzing the function chaperones that regulate antigen presentation; enzymes that ... Læs mere

1.681,32 kr.

This volume discusses the latest advancements in the field of antigen processing and presentation, and the complex assays and techniques that have been devised to help pick apart molecular events along this pathway. The chapters in this book cover topics such as how to make and analyze MHC molecules and their cargo; analyzing the function chaperones that regulate antigen presentation; enzymes that
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Lysosomes

Methods and Protocols

Redigeret af Stephanie M. Hughes, Indranil Basak ...

Serier serie Springer Protocols
This volume provides a wide range of methods to discover and assess lysosome structure and function are provided. Chapters detail the essential methodologies and giving expert insight to key features with notes pertaining to key elements of the methods.Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions tothe material, lists of ... Læs mere

1.868,14 kr.

This volume provides a wide range of methods to discover and assess lysosome structure and function are provided. Chapters detail the essential methodologies and giving expert insight to key features with notes pertaining to key elements of the methods.Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions tothe material, lists of
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Oncogene-Induced Senescence

Methods and Protocols

Redigeret af Vassilis G. Gorgoulis, Maria Cavinato, Konstantinos Evangelou ...

Serier serie Springer Protocols
This volume explores a collection of the most recent experimental approaches for the detection, modelling, characterization, and in-depth computational analysis of the biological phenomenon known as Oncogene-Induced Senescence (OIS). The chapters in this book cover topics such as protocols to study OIS in two-dimensional, three-dimensional, and in vivo (animal and human) settings; analyzing OIS ... Læs mere

2.325,95 kr.

This volume explores a collection of the most recent experimental approaches for the detection, modelling, characterization, and in-depth computational analysis of the biological phenomenon known as Oncogene-Induced Senescence (OIS). The chapters in this book cover topics such as protocols to study OIS in two-dimensional, three-dimensional, and in vivo (animal and human) settings; analyzing OIS
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Recombinant Glycoproteins

Methods and Protocols

Redigeret af Steven B. Bradfute ...

Serier serie Springer Protocols
This detailed volume explores the production and purification of glycoproteins for therapeutic use or basic research, as well as the necessary in-depth understanding of cellular and acellular systems available for these purposes, their advantages and disadvantages, and considerations for choosing the most appropriate system for the desired application. Beginning with chapters on viral-encoded ... Læs mere

1.222,11 kr.

This detailed volume explores the production and purification of glycoproteins for therapeutic use or basic research, as well as the necessary in-depth understanding of cellular and acellular systems available for these purposes, their advantages and disadvantages, and considerations for choosing the most appropriate system for the desired application. Beginning with chapters on viral-encoded
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The ESCRT Complexes

Methods and Protocols

Redigeret af Emmanuel Culetto, Renaud Legouis ...

Serier serie Springer Protocols
This detailed collection gathers both established and recent technical procedures to study the Endosomal Sorting Complex Required for Transport (ESCRT) complexes in a wide range of biological systems: Archaea, A. thaliana, U. maydis, S. cerevisiae, S. pombe, C. elegans, D. melanogaster, and mammalian cells. Opening with a section on imaging techniques, the book continues with chapters covering ... Læs mere

934,02 kr.

This detailed collection gathers both established and recent technical procedures to study the Endosomal Sorting Complex Required for Transport (ESCRT) complexes in a wide range of biological systems: Archaea, A. thaliana, U. maydis, S. cerevisiae, S. pombe, C. elegans, D. melanogaster, and mammalian cells. Opening with a section on imaging techniques, the book continues with chapters covering
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Translational Research Methods for Tauopathies

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Zebrafish

In Vitro Bioassay Techniques for Anticancer Drug Discovery and Development

Proteases and Cancer

Macrophage Migration Inhibitory Factor

Immune Mediators in Cancer

Peptide Antibodies

Antigen Processing and Presentation

Lysosomes

Oncogene-Induced Senescence

Recombinant Glycoproteins

The ESCRT Complexes