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  • Circular RNAs

    Serier serie Springer Protocols
    This second edition details new and updated methods on circular RNA. Chapter guide readers through circular RNA purification, in silico characterization, circRNA detection, sequence validation, quantification , techniques related to gain- and loss-of-function approaches, circular RNA synthesis, split ligation, engineering, nanoparticle packaging, RNA modifications on circular RNA biogenesis, RNA ... Læs mere

    1.798,99 kr.

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  • Drosophila

    Methods and Protocols

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    Serier serie Springer Protocols
    This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and ... Læs mere

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  • Mass Spectrometry-Based Proteomics

    Redigeret af Kris Gevaert ...
    Serier serie Springer Protocols
    This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by ... Læs mere

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  • Alternative Splicing

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    Redigeret af Peter Scheiffele, Oriane Mauger ...
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    This detailed volume collects commonly used and cutting-edge methods to analyze alternative splicing, a key step in gene regulation. After an introduction of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in complex biological systems, visualizing and localizing alternative spliced ... Læs mere

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  • MicroRNA Detection and Target Identification

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    This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe ... Læs mere

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  • Transcription Factor Regulatory Networks

    Redigeret af Qi Song, Zhipeng Tao ...
    Serier serie Springer Protocols
    This book covers various state-of-the-art techniques regarding the associations between transcription factors (TFs) and genes, with a focus on providing methodological and practical references for researchers. The contents cover diverse protocols and summaries of TFs including screening of TF-DNA interactions, detection of open chromatin regions, identification of epigenetic regulations, ... Læs mere

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  • Mammalian Synthetic Systems

    Redigeret af Francesca Ceroni, Karen Polizzi ...
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    This volume details cutting-edge methods that enables engineering of novel functions in mammalian cells. Chapters guide readers through cell-to-cell communication, cell fate control, protein and RNA-based biosensors together with tools for more reliable and faster mammalian genome editing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions ... Læs mere

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  • Microproteins

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    This detailed volume brings together cutting-edge methods from proteomics, transcriptomics, bioinformatics, and functional genomics in order to capture the dynamic and multidisciplinary nature of the microprotein field. Divided into four sections, the book explores the detection and identification of microproteins; transcriptome-based discovery; computational, structural, and evolutionary insights ... Læs mere

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    Serier serie Springer Protocols
    This comprehensive book covers RNA-RNA and RNA-DNA interactions from experimental techniques to information technology. Chapter detail experimental approaches to study RNA-RNA and RNA-DNA Interactions and computational approaches to STUDY RNA-RNA and RNA-DNA Interactions. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions tothe ... Læs mere

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  • Activity-Based Proteomics

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    This volume comprehensively covers techniques that use activity-based proteomics to investigate biological functions of proteins such as lipolytic enzymes, serine hydrolases, chitinases, and glycoside hydrolases. The chapters in this book cover a wide range of protocols and an overview on the application of activity-based proteomics profiling to study deISGylating enzymes, serine hydrolases, ... Læs mere

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  • Directed Evolution

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    This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using ... Læs mere

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  • Deadenylation

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    This volume provides new approaches and technologies into roles of poly(A) metabolism in translation, RNA stability, and quality control of gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and ... Læs mere

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