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  • Activity-Based Proteomics

    Methods and Protocols

    Redigeret af Zhipeng Tao ...
    Serier serie Springer Protocols
    This volume comprehensively covers techniques that use activity-based proteomics to investigate biological functions of proteins such as lipolytic enzymes, serine hydrolases, chitinases, and glycoside hydrolases. The chapters in this book cover a wide range of protocols and an overview on the application of activity-based proteomics profiling to study deISGylating enzymes, serine hydrolases, ... Læs mere

    2.138,77 kr.

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  • Mass Spectrometry-Based Proteomics

    Redigeret af Kris Gevaert ...
    Serier serie Springer Protocols
    This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by ... Læs mere

    1.414,11 kr.

  • Golgi

    Methods and Protocols

    Serier serie Springer Protocols
    This volume provides readers with a collection of new and classical methods, techniques, and applications used to address enduring questions about the structure and functions of the Golgi complex. The chapters in this volume cover diverse topics ranging from model systems; live and fixed cell imaging techniques; in vitro biochemical reconstitution systems; and specific methods developed to study ... Læs mere

    1.756,86 kr.

  • Proteomics for Drug Discovery

    Redigeret af Josip Blonder ...
    Serier serie Springer Protocols
    This detailed volume explores a wide range of experimental and bioinformatic proteomics methods applicable to the drug discovery process. Focused on drug target discovery, validation, characterization, and quantitation, the book delves into bottom-up data-dependent (DDA) shotgun and data-independent acquisition (DIA) proteomics, cell surface proteomics, proteogenomics, chemoproteomics, ... Læs mere

    1.214,06 kr.

  • Circular RNAs

    Serier serie Springer Protocols
    This second edition details new and updated methods on circular RNA. Chapter guide readers through circular RNA purification, in silico characterization, circRNA detection, sequence validation, quantification , techniques related to gain- and loss-of-function approaches, circular RNA synthesis, split ligation, engineering, nanoparticle packaging, RNA modifications on circular RNA biogenesis, RNA ... Læs mere

    1.798,99 kr.

  • Sample Preparation in Biological Mass Spectrometry

    Serier serie Chemistry and Material Science (R0)
    The aim of this book is to provide the researcher with important sample preparation strategies in a wide variety of analyte molecules, specimens, methods, and biological applications requiring mass spectrometric analysis as a detection end-point. In this volume we have compiled the contributions from several laboratories which are employing mass spectrometry for biological analysis. With the ... Læs mere

    1.867,83 kr.

  • Tunable Hydrogels

    Smart Materials for Biomedical Applications

    Serier serie Chemistry and Material Science (R0)
    This book reviews the current knowledge on tunable hydrogels, including the range of different materials and applications, as well as the existing challenges and limitations in the field. It covers various aspects of the material design, particularly highlighting biological responsiveness, degradability and responsiveness to external stimuli.In this book, readers will discover original research ... Læs mere

    3.539,72 kr.

  • Protein Tyrosine Phosphatases

    Methods and Protocols

    Serier serie Springer Protocols
    This second edition volume expands on the previous edition with discussions on the latest advancements in protein tyrosine phosphatases (PTP) research used to investigate these essential enzymes and new inhibitors. The new techniques covered in the chapters of this book include studying enzymes in vitro, in cells, and in animal models through proteomics, genomics, and structural biology. ... Læs mere

    1.335,49 kr.

  • Single Cell ‘Omics of Neuronal Cells

    Serier serie Springer Protocols
    This volume discusses the latest techniques used in the diverse fields of single cell ‘omics and covers topics such as quantifying the single cell transcriptome; isolation of cells in nanoliter volumes for single cell proteomics measurements by nano-LC-MS/MS; and single cell protein characterization by immunoblotting. A wide range of methodologies are highlighted, ranging from high-yield chemical ... Læs mere

    1.400,85 kr.

  • HDAC/HAT Function Assessment and Inhibitor Development

    Methods and Protocols

    Redigeret af Oliver H. Krämer ...
    Serier serie Springer Protocols
    This fully updated edition provides a series of methods for how best to assess functions of histone deacetylases and acetyltransferases. The disease-relevance of dysregulated protein deacetylation by overexpressed or aberrantly activated histone deacetylases has spurred an intense search for novel and improved inhibitors of these enzymes, as reflected in this collection. Expert contributors ... Læs mere

    1.587,64 kr.

  • Proximity Labeling

    Methods and Protocols

    Redigeret af Murat Sunbul, Andres Jäschke ...
    Serier serie Springer Protocols
    This book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived reactive species to non-specifically label molecules within a certain radius of up to twenty nanometers. Beginning with peroxidase-based proximity ... Læs mere

    1.120,66 kr.

  • Mitochondrial Medicine

    Volume 2: Assessing Mitochondria

    Redigeret af Volkmar Weissig, Marvin Edeas ...
    Serier serie Springer Protocols
    This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume II guides readers through chapters on mitochondrial dysfunction, functional’ mitochondria, mitochondrial retrograde, mitochondrial dNTP pool quantification, mitochondrial ADP-ribosylation, blue-native gel approach, 3D ... Læs mere

    942,61 kr.