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  • Protein Post-Translational Modifications

    Methods and Protocols

    Redigeret af Xu Zhang, Rui Chen, Jianjun Li ...
    Serier serie Springer Protocols
    This detailed volume explores robust analytical workflows for post-translational modification (PTM) analysis in both basic and translational contexts. After brief foundational reviews of the field, the book delves into detailed experimental and computational protocols for traditional bottom-up strategies and top-down proteoform analysis, as well as PTM analysis of biotherapeutic proteins, ... Læs mere

    1.681,32 kr.

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  • Mass Spectrometry-Based Proteomics

    Redigeret af Kris Gevaert ...
    Serier serie Springer Protocols
    This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by ... Læs mere

    1.571,37 kr.

  • Cryo-Electron Microscopy in Structural Biology

    From Structural Insights to Tomography and Drug Discovery

    Redigeret af Krishnarao Appasani ...
    Cryo-electron microscopy, in combination with tomography, has emerged as a new technology for visualizing molecular structures at a resolution beyond even 1 Å. Using this technology has revealed the native molecular details of viruses, membranes, enzymes, ribosomes, and cells. This comprehensive volume brings together authoritative overviews of these methods from structural and biological ... Læs mere

    2.130,92 kr.

  • Activity-Based Proteomics

    Methods and Protocols

    Redigeret af Zhipeng Tao ...
    Serier serie Springer Protocols
    This volume comprehensively covers techniques that use activity-based proteomics to investigate biological functions of proteins such as lipolytic enzymes, serine hydrolases, chitinases, and glycoside hydrolases. The chapters in this book cover a wide range of protocols and an overview on the application of activity-based proteomics profiling to study deISGylating enzymes, serine hydrolases, ... Læs mere

    2.139,13 kr.

  • Circular RNAs

    Serier serie Springer Protocols
    This second edition details new and updated methods on circular RNA. Chapter guide readers through circular RNA purification, in silico characterization, circRNA detection, sequence validation, quantification , techniques related to gain- and loss-of-function approaches, circular RNA synthesis, split ligation, engineering, nanoparticle packaging, RNA modifications on circular RNA biogenesis, RNA ... Læs mere

    1.999,20 kr.

  • Structural Genomics and Drug Discovery

    Methods and Protocols

    Redigeret af Wayne F. Anderson ...
    Serier serie Springer Protocols
    Structural Genomics and Drug Discovery: Methods and Protocols focuses on high throughput structure determination methods and how they can be applied to lay the groundwork for structure aided drug discovery. The methods and protocols that are described can be applied in any laboratory interested in using detailed structural information to advance the initial stages of drug discovery. Written in the ... Læs mere

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  • Cell-Wide Identification of Metabolite-Protein Interactions

    Serier serie Springer Protocols
    This thorough volume explores protocols of proteome- and metabolome-wide strategies for the identification of protein-small molecule complexes in different organisms, in order to shed light on these important regulatory interactions. Experimental and computational strategies to characterize protein-metabolite interactions are discussed, and recent advances in enabling technologies are featured as ... Læs mere

    934,02 kr.

  • The Proteomics Protocols Handbook

    Redigeret af John M. Walker ...
    Serier serie Springer Protocols
    Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying ... Læs mere

    2.699,60 kr.

  • Proteomics for Drug Discovery

    Redigeret af Josip Blonder ...
    Serier serie Springer Protocols
    This detailed volume explores a wide range of experimental and bioinformatic proteomics methods applicable to the drug discovery process. Focused on drug target discovery, validation, characterization, and quantitation, the book delves into bottom-up data-dependent (DDA) shotgun and data-independent acquisition (DIA) proteomics, cell surface proteomics, proteogenomics, chemoproteomics, ... Læs mere

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  • Protein Chromatography

    Methods and Protocols

    Serier serie Springer Protocols
    This third edition expands on the previous editions with updated and new chapters on protein chromatography. Chapters detail protein stability and storage, avoiding proteolysis, protein quantitation methods, generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins. Written in the format of the highly successful Methods in Molecular Biology ... Læs mere

    1.484,03 kr.

  • Proteomics in Systems Biology

    Methods and Protocols

    Redigeret af Jennifer Geddes-McAlister ...
    Serier serie Springer Protocols
    This detailed book highlights the diverse techniques and applications of proteomics in an accessible, informative, and concise manner. The collection features sample preparation from distinct extraction, quantification, enrichment, modification, as well as interactome methodology for the in-depth exploration of biological systems, and the application of proteomics to clinical, infectious disease, ... Læs mere

    1.587,91 kr.

  • MicroRNA Detection and Target Identification

    Methods and Protocols

    Redigeret af Tamas Dalmay ...
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    This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe ... Læs mere

    1.134,86 kr.